Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	842,639	C→T	A103V (GCG→GTG)	rlmF →	23S rRNA m(6)A1618 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	842,639	0	C	T	100.0%	150.8 / NA	45	A103V (GCG→GTG)	rlmF	23S rRNA m(6)A1618 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/28);  total (17/28)

GTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATC  >  NC_000913/842505‑842772                                                                                                                                       |                                                                                                                                      gTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATg                                                                                                                                  <  1:770534/140‑1 (MQ=255) gTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATg                                                                                                                                  <  1:482586/140‑1 (MQ=255)    gCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCa                                                                                                                               <  1:597932/140‑1 (MQ=255)     cTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAg                                                                                                                              >  2:741262/1‑140 (MQ=255)     cTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAg                                                                                                                              >  1:444704/1‑140 (MQ=255)          ccATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTc                                                                                                                         >  1:173271/1‑140 (MQ=255)               tttACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGat                                                                                                                    <  2:513890/140‑1 (MQ=255)                 tACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGatat                                                                                                                  <  2:524363/140‑1 (MQ=255)                 tACGCCGTAGCGAACAGGGATACCCTCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGatat                                                                                                                  <  1:302255/140‑1 (MQ=255)                    gCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCgg                                                                                                               >  2:491263/1‑140 (MQ=255)                             aaCTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGc                                                                                                      <  1:771272/140‑1 (MQ=255)                              aCTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCg                                                                                                     <  1:171504/140‑1 (MQ=255)                              aCTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCg                                                                                                     <  2:173271/140‑1 (MQ=255)                              aCTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCg                                                                                                     <  2:152188/140‑1 (MQ=255)                              aCTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCg                                                                                                     <  2:544292/140‑1 (MQ=255)                                tGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTTTATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCg                                                                                                             <  2:620025/130‑1 (MQ=255)                                tGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTTTATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCg                                                                                                             >  1:620025/1‑130 (MQ=255)                                tGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa                                                                                                   <  1:203458/140‑1 (MQ=255)                                tGGGATATCCCCGACAGTTTTCTCTCCCCCCCAGTACTGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa                                                                                                   <  1:300194/140‑1 (MQ=255)                                  ggATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACt                                                                                                 >  2:35540/1‑140 (MQ=255)                                   gATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTg                                                                                                <  1:35540/140‑1 (MQ=255)                                        ccccGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTAttt                                                                                           >  1:199143/1‑140 (MQ=255)                                             gcGGTTTTCTCTGCCTACCAGTACCCGGCCGGGCGGAATATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCg                                                                                      <  1:243202/139‑1 (MQ=255)                                             aCGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCg                                                                                      <  1:741262/140‑1 (MQ=255)                                                      tctGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGc                                                                             <  1:202332/140‑1 (MQ=255)                                                         gCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTa                                                                          <  1:776927/140‑1 (MQ=255)                                                         gCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATACTGGATATTGGCCTTGCTGCGAACTGTATTAATCCGCTGATTGGCGtc                                                                          >  2:243202/1‑139 (MQ=255)                                                          cccACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTAttt                                                                                           <  2:574159/122‑1 (MQ=255)                                                          cccACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTAttt                                                                                           >  1:574159/1‑122 (MQ=255)                                                          cccACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTAc                                                                         <  2:57478/140‑1 (MQ=255)                                                                    ccgggccgggCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATGTATACGATGATTGTCGTACATGAATAtgg                                                               >  1:245181/1‑140 (MQ=255)                                                                      gggccgggcGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATAtggct                                                             <  2:336326/140‑1 (MQ=255)                                                                           gggcGGATTATATTCCTCACCTTGCCGATTTACTGGCTGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGt                                                        <  1:361054/140‑1 (MQ=255)                                                                            ggcGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTTCGAACTGTATTTGTTCACTGATTGGAGTACGTGAATATGGCTGGCGtt                                                       >  2:362894/1‑140 (MQ=255)                                                                            ggcGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGtt                                                       >  1:452368/1‑140 (MQ=255)                                                                                     tatTCATCACCTTGCCGCTTTATTGGCAGAACCAACCGGTACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTa                                              <  2:245181/140‑1 (MQ=255)                                                                                           tcaCCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGaaa                                        >  2:52708/1‑140 (MQ=255)                                                                                                 tGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACcagca                                  >  1:114987/1‑140 (MQ=255)                                                                                                    cGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGcc                               <  1:52708/140‑1 (MQ=255)                                                                                                                aGAAGCAAGCGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCa                   >  2:122070/1‑140 (MQ=255)                                                                                                                         cGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAgg          >  2:194128/1‑140 (MQ=255)                                                                                                                         cGGAACAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAgg          <  1:194128/140‑1 (MQ=255)                                                                                                                              cAATTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGAtt     >  2:201840/1‑140 (MQ=255)                                                                                                                                aTTCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTAt   <  2:114987/140‑1 (MQ=255)                                                                                                                                 ttCCAGTGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATc  <  2:444704/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATC  >  NC_000913/842505‑842772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑