Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	847,173	C→T	intergenic (‑54/+85)	glnP ← / ← glnH	L‑glutamine ABC transporter membrane subunit/L‑glutamine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	847,173	0	C	T	100.0%	103.4 / NA	31	intergenic (‑54/+85)	glnP/glnH	L‑glutamine ABC transporter membrane subunit/L‑glutamine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

CAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGT  >  NC_000913/847051‑847289                                                                                                                           |                                                                                                                     caGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTgcgc                                                                                                     >  1:755274/1‑140 (MQ=255)     gTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCt                                                                                                 <  2:784412/140‑1 (MQ=255)     gTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCt                                                                                                 <  2:581179/140‑1 (MQ=255)              gctccTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAAt                                                                                        >  2:522440/4‑140 (MQ=255)              gCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAAt                                                                                        >  1:623014/1‑140 (MQ=255)              gCACCTTCAATCAGAAGAGGAATGGCAGGCCCGATTGCACTCCAGTCATACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAAt                                                                                        <  2:259169/140‑1 (MQ=255)               cACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATc                                                                                       <  1:194497/140‑1 (MQ=255)                 ccTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCct                                                                                     <  2:755274/140‑1 (MQ=255)                  cTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCctc                                                                                    >  1:486611/1‑140 (MQ=255)                            aaGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAAt                                                                                        >  1:420089/1‑126 (MQ=255)                            aaGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAAt                                                                                        <  2:420089/126‑1 (MQ=255)                            aaGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAgg                                                                          >  2:626451/1‑140 (MQ=255)                            aaGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAg                                                                           >  1:414870/1‑139 (MQ=255)                            aaGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAg                                                                           <  2:414870/139‑1 (MQ=255)                             aGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAggg                                                                         >  1:778133/1‑140 (MQ=255)                               cGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGga                                                                       <  2:486611/140‑1 (MQ=255)                                ggAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGgag                                                                      <  2:778133/140‑1 (MQ=255)                                  aaTGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTGGCGCCCCGCACGCTGATCCTCGCCCCATAGGGGagag                                                                    >  1:212610/1‑140 (MQ=255)                                          gCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGc                                                            >  1:63202/1‑140 (MQ=255)                                               aTGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTg                                                       <  1:613775/140‑1 (MQ=255)                                                      tCCAGTCAAACTGCATACGTTGATCCTGTTACCGTAGTGCAAATAATCTAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTa                                                <  2:212610/140‑1 (MQ=255)                                                               aCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAg                                       <  1:522440/140‑1 (MQ=255)                                                                  gCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGAGAGAGGGCACTGCCAGTGCGTTTTACAGGTGTAGCGt                                    >  1:304345/1‑140 (MQ=255)                                                                                 gTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCa                     <  1:669193/140‑1 (MQ=255)                                                                                 gTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCa                     >  1:467536/1‑140 (MQ=255)                                                                                        tggtgTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGa              >  2:545711/1‑140 (MQ=255)                                                                                         ggtgTAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGaa             >  2:432564/1‑140 (MQ=255)                                                                                           tgtAAATAATCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAAcc           <  1:545711/140‑1 (MQ=255)                                                                                               aataatCAAATTCAGGGGAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAttt       >  2:718219/1‑140 (MQ=255)                                                                                                    tCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTGGGTTCAGTAGCGAACCATTTTTTGt  >  1:269203/1‑140 (MQ=255)                                                                                                    tCAAATTCAGGGTAATTAATGGCTCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAACGTTATTCTTTCGGATCAGTATCGAACCATTTTTTGt  >  1:379385/1‑140 (MQ=255)                                                                                                                           |                                                                                                                     CAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGT  >  NC_000913/847051‑847289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑