| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 878,179 | A→G | intergenic (‑144/‑69) | rimO ← / → bssR | ribosomal protein S12 methylthiotransferase RimO/regulator of biofilm formation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 878,179 | 0 | A | G | 97.1% | 111.8 / NA | 35 | intergenic (‑144/‑69) | rimO/bssR | ribosomal protein S12 methylthiotransferase RimO/regulator of biofilm formation |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (14/20); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTCAGTTACAGGAAAGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGACAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCGATCTGCTGAACCGGTTGATCGACGCACGCGTTGACCTCGCC > NC_000913/878047‑878313 | tatTCAGTTACAGGAAAGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCAtt < 2:611148/140‑1 (MQ=255)tatTCAGTTACAGGAAAGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCAtt < 2:521194/140‑1 (MQ=255) aGTTACAGGAAAGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCt < 2:54743/140‑1 (MQ=255) gTTACAGGAAAGGTCAGGGCAGCGATTCTACTGAGTTCTTGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTc < 2:305838/140‑1 (MQ=255) aaGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAg < 2:149002/140‑1 (MQ=255) aGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGc < 1:106254/140‑1 (MQ=255) agggcagggATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATAc > 2:829471/1‑140 (MQ=255) ggATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTaa > 1:809099/1‑140 (MQ=255) ggATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTAGGCAGCAATTTCCTAAAAAAAAAGCCATACTATTTaa > 1:260329/1‑140 (MQ=255) tACAGAGTTCTGGATAAAATTTTTATCGCAACCTCCTTCACTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGcaa < 2:260329/140‑1 (MQ=255) agagTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGcaacaa < 1:449875/140‑1 (MQ=255) agTTCTGGATAAAATTTTTATCTCAATCTCATTCGCTGGCGGAGGCGATGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAgg < 2:265710/140‑1 (MQ=255) ttCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCt > 2:467840/1‑140 (MQ=255) ttCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGACAGCATTTAGCTTAAACAATAGCCACACTAGTTAATTGCAACAAGGCt > 2:284930/1‑140 (MQ=255) cTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTgg > 1:511199/1‑140 (MQ=255) gATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAACAAATAGCTATAATAATTAATTTCAACAAGGCTGGGaa > 1:265710/1‑140 (MQ=255) aaaaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAagag > 2:668763/1‑140 (MQ=255) aaaaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAagag > 2:489593/1‑140 (MQ=255) aaaaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAagag < 1:467840/140‑1 (MQ=255) aaaaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGCAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATGCTAATTGATTGCAACAAGGCTGGGAagag > 1:253741/1‑140 (MQ=255) aaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAgagga > 1:569787/1‑140 (MQ=255) aaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAgagga > 2:6953/1‑140 (MQ=255) aaTTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAgagga > 1:767337/1‑140 (MQ=255) tGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGAt < 1:829471/140‑1 (MQ=255) ctcATTCGCTGGCGGAGTCGAAGGAAATGTATATTTTGTTAATTCGGCATGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGtt < 2:253741/140‑1 (MQ=255) ctcATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGtt < 2:767337/140‑1 (MQ=255) ctcATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGtt < 1:6953/140‑1 (MQ=255) ttCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGtt < 1:489593/140‑1 (MQ=255) gCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTgac < 2:809099/140‑1 (MQ=255) cGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTgacagaca < 1:668763/140‑1 (MQ=255) gCGAAGGAAATGTAAATTTTGTCAATTCGGCGTGAAGAACTGATCCTGAGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGaa < 2:277135/140‑1 (MQ=255) aaGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCg < 2:511199/140‑1 (MQ=255) tGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCGATctgcc > 2:756986/1‑139 (MQ=255) aaTTTTGTTAATTCGGCGTGAAGAATTGATCCTGGGCGGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCGATCTGCTGAAc < 1:284930/140‑1 (MQ=255) aTCCTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCGATCTGCTGAACCGGTTGATCGACGCACGCGTTGACCTcg > 2:463319/1‑140 (MQ=255) ccTGGGCAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCGATCTGCTGAACCGGTTGATCGACGCACGCGTTGACCTcgcc > 1:587249/1‑140 (MQ=255) | TATTCAGTTACAGGAAAGGTCAGGGCAGGGATTCTACAGAGTTCTGGATAAAATTTGTATCGCAATCTCATTCGCTGGCGGAGGCGAAGGAAATGTAAATTTTGTTAATTCGGCGTGAAGAATTGATCCTGGACAGCATTTTGCTCAAAAAATAGCCATACTATTTAATTGCAACAAGGCTGGGAAGAGGAGGATCGAAGTATGTTCGTTGACAGACAGCGAATCGATCTGCTGAACCGGTTGATCGACGCACGCGTTGACCTCGCC > NC_000913/878047‑878313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |