Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	883,336	T→C	D18G (GAC→GGC)	ybjG ←	undecaprenyl pyrophosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	883,336	0	T	C	100.0%	115.2 / NA	33	D18G (GAC→GGC)	ybjG	undecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

ATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAA  >  NC_000913/883204‑883468                                                                                                                                     |                                                                                                                                     aTAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGt                                                                                                                               <  1:140847/140‑1 (MQ=255) aTAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGt                                                                                                                               >  2:569314/1‑140 (MQ=255)  tAGCGATTTTTATCTCCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAATACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTc                                                                                                                              <  2:157405/140‑1 (MQ=255)    gCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTcgc                                                                                                                            <  1:617092/140‑1 (MQ=255)         ttttATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGGGATCATCCACGGAGCCGAGCCTGGCGTCGCGTtaa                                                                                                                       <  2:139483/140‑1 (MQ=255)             aTCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAag                                                                                                                   <  2:570362/140‑1 (MQ=255)               cACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAagag                                                                                                                 <  1:578576/140‑1 (MQ=255)                aCCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGTAGCCGAGCCTGGCGTCGCGTTAATAagaga                                                                                                                <  1:810083/140‑1 (MQ=255)                aCCAGTTGCCGTTGTGCAGTAAGCCCCCACAGCCAAAGTACCACGGCCGGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAagaga                                                                                                                <  2:289743/140‑1 (MQ=255)                               gCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGAtt                                                                                                 >  1:1670/1‑140 (MQ=255)                                   tAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCaaa                                                                                             <  1:512879/140‑1 (MQ=255)                                        ccccACAGCCAAAGTACCATGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTc                                                                                        >  2:471571/1‑140 (MQ=255)                                          ccACAACCAAAGTGCCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATGGAGAGAGATTCAAATTTTCCa                                                                                      <  1:469807/140‑1 (MQ=255)                                                        ccACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCACATTTTCCAGCATAACTTCCCGa                                                                        >  1:324929/1‑140 (MQ=255)                                                          aCGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAAGAAGAGAGAATAGAGAGCGATTCAAAGTTTCGAGCATAACTTCCCGACg                                                                      >  2:240377/1‑140 (MQ=255)                                                            ggCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATCGAGAGAGATTCAAATTTTCCAGCATATCTTCCCGACGCa                                                                    <  2:808784/140‑1 (MQ=255)                                                            ggCCAGCAACGGCACAACGGTAATCAAATTTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCa                                                                    <  1:256629/140‑1 (MQ=255)                                                             gCCAGCAACGGCACCGCGGTAACCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCaa                                                                   <  1:263988/140‑1 (MQ=255)                                                              ccAGCAACGGCACCACGGTAATCAACTCTCTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCaaa                                                                  <  1:210657/140‑1 (MQ=255)                                                              ccAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATGCAAATTTTTCAGCGTAACTTCCCGACGCaaa                                                                  >  2:210657/1‑140 (MQ=255)                                                                aGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGt                                                                >  2:27586/1‑140 (MQ=255)                                                                  cAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACAGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGa                                                              >  2:88677/1‑140 (MQ=255)                                                                             cGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAg                                                   <  2:324383/140‑1 (MQ=255)                                                                                      aaTCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAg                                          <  1:569314/140‑1 (MQ=255)                                                                                                  tAAAAATCGCCAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTga                              >  1:692440/1‑140 (MQ=255)                                                                                                           ccAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGt                     <  2:1670/140‑1 (MQ=255)                                                                                                            cAACGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTg                    <  1:186627/140‑1 (MQ=255)                                                                                                             aaCGAGATCATCCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGc                   <  1:785931/140‑1 (MQ=255)                                                                                                                     catcCACGGAGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTaaa           <  1:471571/140‑1 (MQ=255)                                                                                                                        ccACGGAGCCGAGCCTGGCGTCCCGTTAATAAGAGAGCATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTg        <  1:240377/140‑1 (MQ=255)                                                                                                                              aGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTaaa  >  1:741033/1‑140 (MQ=255)                                                                                                                              aGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTaaa  >  2:440068/1‑140 (MQ=255)                                                                                                                              aGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTaaa  >  1:447921/1‑140 (MQ=255)                                                                                                                              aGCCGAGCCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTaaa  >  2:790749/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     ATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAA  >  NC_000913/883204‑883468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑