Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	884,549	T→C	W293R (TGG→CGG)	mdfA →	multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	884,549	0	T	C	100.0%	126.7 / NA	39	W293R (TGG→CGG)	mdfA	multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/18);  total (21/18)

TACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTA  >  NC_000913/884413‑884683                                                                                                                                         |                                                                                                                                       tACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTTTGACCTCGCGCCGCACAGTACGTTCCCTGATTATTATGGGcggccggc                                                                                                                                     >  2:349663/1‑140 (MQ=255)    cGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGcggccggc                                                                                                                                     <  2:515687/137‑1 (MQ=255)    cGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGcggccggc                                                                                                                                     >  1:515687/1‑137 (MQ=255)       cGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTa                                                                                                                               >  1:31942/1‑140 (MQ=255)         aGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGATGAGTAGTATGGGAGGCCGGCCGATTATg                                                                                                                             >  1:222580/1‑140 (MQ=255)           cAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGAt                                                                                                                           <  1:738233/140‑1 (MQ=255)                   cagcTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCATACGTTCGCTGACTCTTATGGGCGGCCGGCCCATTATGATTGGTCTAt                                                                                                                   >  2:352435/1‑140 (MQ=255)                        aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCAGCCTGCTGATTATGATTGGTCTATTGGTc                                                                                                              >  1:379856/1‑140 (MQ=255)                        aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGATCGCTGATGATTATGGCCGGCCGGCAGATTTTGATTGGTCTATTGGTc                                                                                                              >  2:252836/1‑140 (MQ=255)                          gAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCgc                                                                                                            <  2:31942/140‑1 (MQ=255)                                ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCtgctgc                                                                                                      >  2:721207/1‑140 (MQ=255)                                ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCtgctgc                                                                                                      >  2:494275/1‑140 (MQ=255)                                ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGACGGACAGACGATTATGGTTGGATTATTGGTAGCtgctgc                                                                                                      >  2:400046/1‑140 (MQ=255)                                  cTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCgg                                                                                                    >  2:43385/1‑140 (MQ=255)                                   ttGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGTCCGGCCGCTTATGATTTGTCTGTTGGTCGCTGCTGCGGc                                                                                                   >  2:300047/1‑140 (MQ=255)                                     gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCaa                                                                                                 >  2:670264/1‑140 (MQ=255)                                         cAAGTGCCTATTTTCGGCGCGTGAATTGCGGCTAACTTGTTGTTAGCGTGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGt                                                                                             <  2:222580/140‑1 (MQ=255)                                            gTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTAt                                                                                          >  2:543591/1‑140 (MQ=255)                                                 tATTTTCGGGGCGTTCATTGCGAGTCACTGGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCAt                                                                                     <  1:387909/140‑1 (MQ=255)                                                 tATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCAt                                                                                     <  2:153482/140‑1 (MQ=255)                                                 tATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCAt                                                                                     >  1:205629/1‑140 (MQ=255)                                                   ttttCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGTTCGCTGATGCGGCGACGGTTATCTCATCg                                                                                   >  1:286282/1‑140 (MQ=255)                                                     ttCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCa                                                                                 <  1:494275/140‑1 (MQ=255)                                                        ggggCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAcgc                                                                              <  1:543591/140‑1 (MQ=255)                                                          ggCGTTAATTGCGGGTAACTTGCTGCTAGCGCGTCTGCCCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGt                                                                            <  1:245871/140‑1 (MQ=255)                                                                       ggTAACTTGCTGTTAGCGCGGCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGAGGCAACGATTATCTCAACGCAAGCGTATTTATGGATGAc                                                               >  2:317570/1‑140 (MQ=255)                                                                          aaCTTGCTGTTAGCGCGGCTGACCTCGCGCCTCACTGTAAGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGc                                                            <  2:291915/140‑1 (MQ=255)                                                                           aCTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGt                                                                            <  1:792678/123‑1 (MQ=255)                                                                           aCTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGt                                                                            >  2:792678/1‑123 (MQ=255)                                                                           aCTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGcc                                                           <  2:205629/140‑1 (MQ=255)                                                                                 tGTTAGCGCGTCTGACCTCGCGGCGGACCTTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTa                                                     <  1:349663/140‑1 (MQ=255)                                                                                  gTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTaa                                                    <  1:670264/140‑1 (MQ=255)                                                                                          gTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTAt                                            <  2:490970/140‑1 (MQ=255)                                                                                           tCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATAGGCGGCCGTCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATg                                           <  2:334510/140‑1 (MQ=255)                                                                                               aCCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCttt                                       <  1:721207/140‑1 (MQ=255)                                                                                                   cgcgCCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGt                                   >  2:94071/1‑140 (MQ=255)                                                                                                    gcgcCGCACCGTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTa                                  >  2:44435/1‑140 (MQ=255)                                                                                                              gTACGTTCGCTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGc                        <  1:94071/140‑1 (MQ=255)                                                                                                                       cTGATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCCGGTTAAGTATTTATGCTTTCGGTATTCGTCTGGCGAATGCggg               >  1:142710/1‑140 (MQ=255)                                                                                                                         gATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCg                       <  2:16978/130‑1 (MQ=255)                                                                                                                         gATTATTATGGGCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCg                       >  1:16978/1‑130 (MQ=255)                                                                                                                                  gggCGGCCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGAt    >  1:518509/1‑140 (MQ=255)                                                                                                                                    gcggcCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTa  >  1:486233/1‑140 (MQ=255)                                                                                                                                    gcggcCGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTa  >  1:159475/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTA  >  NC_000913/884413‑884683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑