Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	77,723	A→G	T35A (ACA→GCA)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	77,723	0	A	G	100.0%	198.2 / NA	56	T35A (ACA→GCA)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/33);  total (23/33)

GCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGC  >  NC_000913/77600‑77858                                                                                                                            |                                                                                                                                        gccgcGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCt                                                                                                                         <  1:586241/140‑1 (MQ=255) gccgcGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCt                                                                                                                         <  2:781940/140‑1 (MQ=255) gccgcGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCt                                                                                                                         <  1:810812/140‑1 (MQ=255) gccgcGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGCCCGGGGCGCTAAAGGCTCCTGCATTGAGCTTATTTCt                                                                                                                         >  1:297310/1‑140 (MQ=255)                  aCGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGgcg                                                                                                        >  1:600499/1‑140 (MQ=255)                     tATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGgcgcgc                                                                                                     <  2:701710/140‑1 (MQ=255)                       tGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCaa                                                                                                   <  2:429596/140‑1 (MQ=255)                        gATCTGGATAATGACGATGGCTCGCAGTAGGAACGGTTTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAAc                                                                                                  <  1:271793/140‑1 (MQ=255)                         aTCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAAcc                                                                                                 >  2:692816/1‑140 (MQ=255)                              gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTGCTGCATTGGGCTAATTACTGAGTCGTGAGGGTGGCGCGCAACCTTTCt                                                                                            >  2:352110/1‑140 (MQ=255)                              gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTGCTGCATTGAGCTGATTGCTGAGTCGAGAGGCTGGCGCGCAACCTTTCt                                                                                            >  2:240727/1‑140 (MQ=255)                              gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCt                                                                                            >  1:130707/1‑140 (MQ=255)                              gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCt                                                                                            >  1:128209/1‑140 (MQ=255)                              gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCt                                                                                            >  2:166905/1‑140 (MQ=255)                                   tGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATc                                                                                       >  1:37794/1‑140 (MQ=255)                                     aCGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTTTGCTGGTCTCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCgg                                                                                     <  2:353189/140‑1 (MQ=255)                                     aCGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCgg                                                                                     >  2:443938/1‑140 (MQ=255)                                      cGATGGCTCGCCGTATGCACGGCGTTTACGCCGCAATTACGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGc                                                                                    <  2:222764/140‑1 (MQ=255)                                      cGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGc                                                                                    <  1:7673/140‑1 (MQ=255)                                      cGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGc                                                                                    <  1:166905/140‑1 (MQ=255)                                      cGATGGCTCGCCGTATGAACGGTGTGTACACGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGc                                                                                    <  1:304593/140‑1 (MQ=255)                                      cGATGGCTCGCCGTACGAACGGTGTATACTCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGc                                                                                    <  1:347126/140‑1 (MQ=255)                                        aTGGCTCGCCGTATGCACGGTGTTTACGCCGCATTTACGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCct                                                                                  <  1:352110/140‑1 (MQ=255)                                        aTGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCct                                                                                  <  2:824932/140‑1 (MQ=255)                                        aTGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCct                                                                                  <  1:141386/140‑1 (MQ=255)                                        aTGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCct                                                                                  <  2:543636/140‑1 (MQ=255)                                                ccGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTtata                                                                          <  1:52485/140‑1 (MQ=255)                                                ccGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCGTTTATGATAGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTtata                                                                          <  2:285713/140‑1 (MQ=255)                                                 cGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATAc                                                                         <  2:533162/140‑1 (MQ=255)                                                    aTGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGTTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGt                                                                      <  2:315125/140‑1 (MQ=255)                                                    aTGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGt                                                                      <  2:725700/140‑1 (MQ=255)                                                    aTGAACGGTGTTTACGCGGCATCTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGt                                                                      <  2:347052/140‑1 (MQ=255)                                                     tGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTg                                                                     >  1:505970/1‑140 (MQ=255)                                                           gtgtTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAAGTTGGCGCGCAAACTTTCTGGATCGTCCTCTGTTATACGGTGAATGCt                                                               >  2:391865/1‑140 (MQ=255)                                                              tttACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTAtt                                                            >  1:568598/1‑140 (MQ=255)                                                                      gCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTTGATCGTCGTCTTTTATACGGTTAATGCTATTGCTGGGAt                                                    >  2:392366/1‑140 (MQ=255)                                                                      gCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGCTCGGCCTTTTTGATCCGGTGAATGCTATTGCTGGGAt                                                    >  1:281173/1‑140 (MQ=255)                                                                      gCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTGTTTTATCCGGTGAATGCTATTGCTGGGAt                                                    >  1:350349/1‑140 (MQ=255)                                                                      gCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGAt                                                    >  2:551930/1‑140 (MQ=255)                                                                      gCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGAt                                                    <  2:568598/140‑1 (MQ=255)                                                                      gCATTTATGCTGGTCCCTTTTATGATGGGTGTGGCCGTGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGAt                                                    <  1:349207/140‑1 (MQ=255)                                                                      gCATTTATGCTGGTCACTTTTATGACGGGTGTGGCCGAGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGAt                                                    <  1:392366/140‑1 (MQ=255)                                                                                tGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGc                                          <  1:692816/140‑1 (MQ=255)                                                                                  gTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCct                                        <  1:467327/140‑1 (MQ=255)                                                                                  gTCGCTTTTATGAT‑GGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCctc                                       >  1:576497/1‑140 (MQ=255)                                                                                   tCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCct                                        <  1:707375/139‑1 (MQ=255)                                                                                   tCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCct                                        >  2:707375/1‑139 (MQ=255)                                                                                     gCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTg                                     <  2:558811/140‑1 (MQ=255)                                                                                       ttttATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGt                                   >  1:560256/1‑140 (MQ=255)                                                                                                 ggggTGGCCGGGGCGGTACAGGCTCATGCTTTGAGCTCATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACg                         <  1:391865/140‑1 (MQ=255)                                                                                                 ggggTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACg                         <  2:576497/140‑1 (MQ=255)                                                                                                 ggggTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACg                         >  1:619442/1‑140 (MQ=255)                                                                                                 ggggTGGCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGCTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGGAAGCCTCTGGTTGGCAAAACg                         >  2:671920/1‑140 (MQ=255)                                                                                                      ggCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTg                    <  2:560256/140‑1 (MQ=255)                                                                                                       gCCGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGa                   <  1:19861/140‑1 (MQ=255)                                                                                                        ccGGGGCGCTACAGGCTCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGAc                  <  2:505970/140‑1 (MQ=255)                                                                                                                        tCCTGCATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGc  >  1:546137/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                        GCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATTGCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGC  >  NC_000913/77600‑77858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑