Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,214	C→T	intergenic (‑134/+58)	pflA ← / ← pflB	pyruvate formate‑lyase activating enzyme/pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,214	0	C	T	100.0%	145.3 / NA	43	intergenic (‑134/+58)	pflA/pflB	pyruvate formate‑lyase activating enzyme/pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/25);  total (18/25)

ACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAG  >  NC_000913/951077‑951350                                                                                                                                          |                                                                                                                                         aCATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCa                                                                                                                                        >  1:410718/1‑140 (MQ=255)        ggTGTTTCTCCAGATGTGGCCCCTCTGAGGCTGTGGTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGt                                                                                                                                 <  2:325111/140‑1 (MQ=255)        ggTGTTTCTCCAGATGTGGCCCCTCTGAGGCCGTGTTGGTGCGCAGCTAGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGt                                                                                                                                 <  2:353598/140‑1 (MQ=255)        ggTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGt                                                                                                                                 <  1:528830/140‑1 (MQ=255)                    gATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAATAAAGGCCTCACTTTCGTGGAGCCTTTAtc                                                                                                                     >  1:325111/1‑139 (MQ=255)                                         tgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAt                                                                                                >  2:762426/1‑140 (MQ=255)                                         tgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAt                                                                                                >  1:508319/1‑140 (MQ=255)                                         tgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAt                                                                                                >  2:640579/1‑140 (MQ=255)                                           ttGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAttt                                                                                              <  2:458904/140‑1 (MQ=255)                                               tGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGt                                                                                          <  2:508319/140‑1 (MQ=255)                                                 cgcAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCa                                                                                        >  2:187658/1‑140 (MQ=255)                                                   cAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCaaa                                                                                      <  2:31064/140‑1 (MQ=255)                                                   cAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCaaa                                                                                      >  1:478128/1‑140 (MQ=255)                                                     gCTCGAAGGCTACGTTGAGTCTTTTTTGGTAGTCACCGTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATc                                                                                    <  1:261326/140‑1 (MQ=255)                                                     gCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTAGGCTTTTAACTGTACGATTTCAGTCAAATc                                                                                    >  1:353861/1‑140 (MQ=255)                                                       tCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                                                  <  1:640579/140‑1 (MQ=255)                                                       tCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                                                  <  1:105249/140‑1 (MQ=255)                                                         gAAGGCTACGTCGAGTCGGTTTTGGCAGTCATCTTGAAGTATAGATAGATGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAAt                                                                                <  2:353861/140‑1 (MQ=255)                                                           aGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTa                                                                              >  1:31767/1‑140 (MQ=255)                                                             gCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAGGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACa                                                                            <  1:534019/140‑1 (MQ=255)                                                             gCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACa                                                                            <  2:426662/140‑1 (MQ=255)                                                             gCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACa                                                                            <  2:410718/140‑1 (MQ=255)                                                              cTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACAt                                                                           <  2:478128/140‑1 (MQ=255)                                                                aCGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                                                  >  1:695527/1‑131 (MQ=255)                                                                aCGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                                                  <  2:695527/131‑1 (MQ=255)                                                                aCGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTCTACAATCTCAGTCTAATCTAATTACATAg                                                                         >  1:277951/1‑140 (MQ=255)                                                                 cGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGa                                                                        <  2:491490/140‑1 (MQ=255)                                                                           gTTTTGGCAGTCACCTTTAAGTTTAGATAGCTGACAAAACAGGCTCTCTCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAg                                                              <  2:277951/140‑1 (MQ=255)                                                                             tttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGt                                                            <  1:804603/140‑1 (MQ=255)                                                                             tttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGt                                                            <  1:104160/140‑1 (MQ=255)                                                                                 gCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGa                                                                        <  2:567989/124‑1 (MQ=255)                                                                                 gCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGa                                                                        >  1:567989/1‑124 (MQ=255)                                                                                                  tagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctg                                       <  1:187658/140‑1 (MQ=255)                                                                                                   agatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgc                                      >  1:533217/1‑140 (MQ=255)                                                                                                   agatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACAAAGATTGAGTGAAGGTACGAGTGATAACGTCctgctgc                                      >  1:259238/1‑140 (MQ=255)                                                                                                    gatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgct                                     <  1:762426/140‑1 (MQ=255)                                                                                                    gatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgct                                     <  2:533217/140‑1 (MQ=255)                                                                                                         cTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCt                                >  1:802620/1‑140 (MQ=255)                                                                                                          tGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                               >  2:715302/1‑140 (MQ=255)                                                                                                            aCAAAAAAGGCTCTCGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTa                             >  2:793049/1‑140 (MQ=255)                                                                                                                        ctcGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                 <  1:646258/140‑1 (MQ=255)                                                                                                                        ctcGCGCTAAAAAAGGCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTg                 >  1:176064/1‑140 (MQ=255)                                                                                                                                       gCCTCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAg  <  1:22159/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAG  >  NC_000913/951077‑951350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑