Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	952,388	A→G	A389A (GCT→GCC)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	952,388	0	A	G	100.0%	134.6 / NA	38	A389A (GCT→GCC)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/18);  total (20/18)

GCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCG  >  NC_000913/952251‑952523                                                                                                                                          |                                                                                                                                        gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGgc                                                                                                                                       >  1:543211/1‑140 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTGCTCATACAGCAGAGAAGAGGTCTCGATGGACACTTTGgc                                                                                                                                       >  2:240397/1‑140 (MQ=255)                    gTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTc                                                                                                                    >  2:168413/1‑140 (MQ=255)                            aCGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAg                                                                                                            >  1:598205/1‑140 (MQ=255)                              gATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGtt                                                                                                          <  2:598205/140‑1 (MQ=255)                                 catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGttttt                                                                                                       <  2:802849/140‑1 (MQ=255)                                  atcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTc                                                                                                      >  1:32724/1‑140 (MQ=255)                                            cGCAGCAAGCAATAGCGTAGTCATCGTTGTTCAAGTCCGTACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGa                                                                                            <  2:230992/140‑1 (MQ=255)                                                 caagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGt                                                                                       >  2:22587/1‑140 (MQ=255)                                                 caagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGt                                                                                       >  1:726451/1‑140 (MQ=255)                                                  aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTc                                                                                      <  2:578288/140‑1 (MQ=255)                                                               gTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCg                                                                         >  2:764462/1‑140 (MQ=255)                                                               gTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCg                                                                         <  1:764462/140‑1 (MQ=255)                                                                tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCgg                                                                        >  2:798715/1‑140 (MQ=255)                                                                   tCGTTGTTGAAGTCCGGCCGCAACAGGTCACCGTTCTCATACTGCAGATAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGgaga                                                                     <  2:379447/140‑1 (MQ=255)                                                                   tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGgaga                                                                     <  2:32724/140‑1 (MQ=255)                                                                     gttgttGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACg                                                                   >  2:483051/1‑140 (MQ=255)                                                                     gttgttGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACg                                                                   <  1:483051/140‑1 (MQ=255)                                                                        gttgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGAc                                                                <  1:168413/140‑1 (MQ=255)                                                                        gttgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGAc                                                                <  1:22587/140‑1 (MQ=255)                                                                        gttgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACGTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGAc                                                                <  2:464000/140‑1 (MQ=255)                                                                        gttgAAGTCCGGACGCATCAGGACATCGTTCTCATACCGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGAc                                                                <  1:240397/140‑1 (MQ=255)                                                                          tgAAGTCCGGACGCATCTGGTCCTCGTTCTCTTACTGCAAAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGAccc                                                              <  2:339731/140‑1 (MQ=255)                                                                            aaGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCAt                                                            <  2:543211/140‑1 (MQ=255)                                                                            aaGTCCGGACGCATCAGCTCATGGTTCTCATCCTGTAGATAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCAt                                                            <  2:211284/140‑1 (MQ=255)                                                                                                aTCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGa                                        >  2:157651/1‑140 (MQ=255)                                                                                                  cGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGaaa                                      >  1:165399/1‑140 (MQ=255)                                                                                                       tcATACTGCAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGAAGGACCCATGGTGTACAGGGTGTTCAGGAAACGGaa                                 >  2:279108/1‑140 (MQ=255)                                                                                                               cAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGaaa                                      <  2:707070/127‑1 (MQ=255)                                                                                                               cAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGaaa                                      >  1:707070/1‑127 (MQ=255)                                                                                                               cAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACTGAAg                                >  2:632170/1‑133 (MQ=255)                                                                                                               cAGAGAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACTGAAg                                <  1:632170/133‑1 (MQ=255)                                                                                                                agagAAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGttttt                        <  2:165399/140‑1 (MQ=255)                                                                                                                 gagaAGAGGTGTCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTg                       >  2:144208/1‑140 (MQ=255)                                                                                                                         gtgtCGATGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAg               >  2:731242/1‑140 (MQ=255)                                                                                                                            tCGATGGACACTTTGGCGGCGAATTTCTGGAAGTTCAGCGGCAGTTTTTCAGACCAGAGAATGGTCATGTTCGGTTCCGAGGACGGACCCATGGTGTAGAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAgggg            >  2:471522/1‑139 (MQ=255)                                                                                                                                tGGACACTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGa        <  1:798715/140‑1 (MQ=255)                                                                                                                                    cacTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGt    >  1:504479/1‑140 (MQ=255)                                                                                                                                     acTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTc   <  1:731242/140‑1 (MQ=255)                                                                                                                                      cTTTGGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGCGTGTTCAGAAAAAGGACGCTGTTTTGGGTACCCAGGGTACGACCGTCg  >  2:248431/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCG  >  NC_000913/952251‑952523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑