| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 964,208 | A→G | intergenic (+96/‑112) | ihfB → / → ycaI | integration host factor subunit beta/conserved inner membrane protein YcaI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 964,208 | 0 | A | G | 97.4% | 132.6 / NA | 39 | intergenic (+96/‑112) | ihfB/ycaI | integration host factor subunit beta/conserved inner membrane protein YcaI |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (18/20); minor base T (1/0); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGT > NC_000913/964075‑964336 | gTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGttt > 2:562217/1‑140 (MQ=255)gTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGttt > 2:663078/1‑140 (MQ=255) tGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAAGCTGGAACTGGAACCTGGCTCGCAGAGTTTTTAGCAGTTTTCACCCttt > 2:331679/1‑140 (MQ=255) gcgcGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAGGCTGCCTCGCATAGCTTTGAGCAGTTTTCACCCTTTc > 1:377853/1‑140 (MQ=255) cgcgATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCg > 1:116580/1‑140 (MQ=255) cgcgATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCg > 2:623097/1‑140 (MQ=255) cgATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGGTTAGAGCAGATTTCACCCTTTCGtt > 1:283018/1‑140 (MQ=255) tCGCGCCACTATTTACTGTTAAGTTTTTTACTCAAGCTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTaaa < 2:377853/140‑1 (MQ=255) atatTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTg < 1:562217/140‑1 (MQ=255) aCGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATg > 2:813188/1‑140 (MQ=255) gTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCt > 1:662538/1‑140 (MQ=255) ttAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTc > 1:579358/1‑140 (MQ=255) tttACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGATCAGTTTTCACCCATACGGTAAATTCTTCTGATTATGCCTGGGGGAACg > 2:394425/1‑140 (MQ=255) aaaCTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAAtt < 2:579358/140‑1 (MQ=255) aCTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTcc < 2:116580/140‑1 (MQ=255) aCTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTcc < 2:662538/140‑1 (MQ=255) gagagaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGAc < 1:813188/140‑1 (MQ=255) gagagaAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGAc < 1:818396/140‑1 (MQ=255) aaaaGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCg < 1:623097/140‑1 (MQ=255) aaaaGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCg > 1:828720/1‑140 (MQ=255) tGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCg > 2:694194/1‑131 (MQ=255) tGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCg < 1:694194/131‑1 (MQ=255) ggTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGc > 1:174984/1‑140 (MQ=255) gTGCTTTTTTCATTTCTTTAATGTGGAACTGTAAGCTGCTTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGca < 2:283018/140‑1 (MQ=255) gTGCTTTTTTCATTTATCTAATTTGGAACAGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGca < 1:331679/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTGGGGGAACGTAAACTCCCCACCAGACAACCGCTCAACAAAGTTGCacac > 2:363670/1‑140 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCacac < 2:828720/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCacac > 2:697221/1‑140 (MQ=255) tCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCa < 1:697221/140‑1 (MQ=255) tCATTTCTCTAATCTGGAACTGCAAACTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCa < 1:394425/140‑1 (MQ=255) cATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCAt < 1:663078/140‑1 (MQ=255) tttCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATaa < 1:153036/140‑1 (MQ=255) gaactggaaGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTg > 2:6927/1‑140 (MQ=255) aactggaaGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTAGCAGACGTTCCATGAACAGGGAGGGGTGc > 1:240902/1‑140 (MQ=255) ctggaaGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCAt < 1:485473/140‑1 (MQ=255) tggaaGCTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATg < 2:174984/140‑1 (MQ=255) cTGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATa > 2:80897/1‑140 (MQ=255) tGCCTCGCAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATaa > 2:620213/1‑140 (MQ=255) cAGAGTTTTGAGCAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt < 1:363670/140‑1 (MQ=255) cAGAGTTTTGAGCAGATTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt < 1:321853/140‑1 (MQ=255) | GTAAAGAACTGCGCGATCGCGCCAATATTTACGGTTAAGTTTTTTACTCAAACTTGAACGAGAGAAAAGCACCTGTCGGGTGCTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGT > NC_000913/964075‑964336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |