Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,007,316 T→C T95T (ACA→ACG)  ycbX ← 6‑N‑hydroxylaminopurine resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,007,3160TC97.9% 155.9 / ‑4.4 48T95T (ACA→ACG) ycbX6‑N‑hydroxylaminopurine resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (23/23);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

AGGTACAGTGTTGTGGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGTGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTG  >  NC_000913/1007187‑1007444
                                                                                                                                 |                                                                                                                                
agGTACAGTGTTGTGGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCaaa                                                                                                                        >  2:698151/1‑140 (MQ=255)
agGTACAGTGTTGTGGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGACGCAATTCGCGCGGTAAAATGCGTGCCCCaaa                                                                                                                        >  1:48633/1‑140 (MQ=255)
           tgtgGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGt                                                                                                             >  2:10427/1‑140 (MQ=255)
            gtgGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTg                                                                                                            >  2:429264/1‑140 (MQ=255)
             tgGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGc                                                                                                           >  2:543842/1‑140 (MQ=255)
               gCGTTTCACGCGCCGGCACATTAGTGGCCCCACCCCGCGTAATTGCACTTCGCGGGAGAAAAATCCCCTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGTGTGCCCCAAACTTCGGTTGGTGCGt                                                                                                         <  2:360116/140‑1 (MQ=255)
                  tttCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCTCAATTCGCACGGGAAACTGCGTGCCCCAAACTTCGGTTGGTGCGTCtt                                                                                                      >  2:268795/1‑140 (MQ=255)
                  tttCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCtt                                                                                                      >  2:658726/1‑140 (MQ=255)
                    tCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTtgt                                                                                                    >  1:332940/1‑140 (MQ=255)
                    tCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGGAAAATGCTTGCCCCAAACTTCGGTTGGTGCGTCTtgt                                                                                                    >  1:277679/1‑140 (MQ=255)
                           ccGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGa                                                                                             >  2:155846/1‑140 (MQ=255)
                               gTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATc                                                                                         >  1:704017/1‑140 (MQ=255)
                                 cATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAg                                                                                       <  1:155846/140‑1 (MQ=255)
                                   tttGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCa                                                                                     <  2:627583/140‑1 (MQ=255)
                                     tgtgGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCaaa                                                                                   <  1:718060/140‑1 (MQ=255)
                                         gCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGa                                                                               >  2:776298/1‑140 (MQ=255)
                                              acccaGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACata                                                                          <  1:576209/140‑1 (MQ=255)
                                              acccaGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACata                                                                          <  2:704017/140‑1 (MQ=255)
                                                  aGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATgcg                                                                      <  1:10427/140‑1 (MQ=255)
                                                   gCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTGCGGTTGGTGCGTATTGTGTGGCGAAATCAGGAAAACGAACATATgcgc                                                                     >  2:291580/1‑140 (MQ=255)
                                                     gTAATTGCACTTCGCGGGAGAATAATCCACTTAGCCAGTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGGGAAATCAGCAAAACGAACATATGCGCTa                                                                   <  1:268795/140‑1 (MQ=255)
                                                     gTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTa                                                                   <  1:658726/140‑1 (MQ=255)
                                                       aaTTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACt                                                                 <  1:429264/140‑1 (MQ=255)
                                                        aTTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTg                                                                >  1:292248/1‑140 (MQ=255)
                                                         ttGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGc                                                               >  1:427162/1‑140 (MQ=255)
                                                              cTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCt                                                          >  2:475468/1‑140 (MQ=255)
                                                                tCGCGGGAGAAAAATTCACTTAGCCATTTATTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTgg                                                        <  1:302714/140‑1 (MQ=255)
                                                                 cgcgGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGt                                                       >  1:764143/1‑140 (MQ=255)
                                                                 cgcgGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGt                                                       >  1:7953/1‑140 (MQ=255)
                                                                       agaAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTg                                                 <  2:424882/140‑1 (MQ=255)
                                                                                   ttAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATTTGCGCTAGTGCGATCTGGTGCGGTGAGATGCAAGCca                                     >  1:361063/1‑140 (MQ=255)
                                                                                   ttAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCca                                     <  1:58598/140‑1 (MQ=255)
                                                                                    tAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCcat                                    <  2:634668/140‑1 (MQ=255)
                                                                                        cATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATg                                <  1:408416/140‑1 (MQ=255)
                                                                                         aTTTGTTGATCGCGTCTGGCGCGATTCGCGCTGTAGAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGc                               <  2:332940/140‑1 (MQ=255)
                                                                                         aTTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGc                               <  2:7953/140‑1 (MQ=255)
                                                                                          tttGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGca                              >  2:585754/1‑140 (MQ=255)
                                                                                           ttgttgATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGcac                             <  2:427162/140‑1 (MQ=255)
                                                                                                gATCGCGTCTGGCGCCATTCGCTCGTTAAAATGCGTGTCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTg                        <  1:393889/140‑1 (MQ=255)
                                                                                                gATCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTg                        <  2:680993/140‑1 (MQ=255)
                                                                                                gATCGCGTCTGGCGCAATTCGCACGATAAAATGCGTGGCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTg                        <  1:291580/140‑1 (MQ=255)
                                                                                                 aTCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGa                       >  2:442488/1‑140 (MQ=255)
                                                                                                 aTCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGa                       <  1:543842/140‑1 (MQ=255)
                                                                                                  tCGCGTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGaa                      <  1:647735/140‑1 (MQ=255)
                                                                                                     cgTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTGCTGCCATCAGGTACGGGGAGATGCAGGCCAACATGCACAGGTGAAGGa                   >  2:229694/1‑140 (MQ=255)
                                                                                                     cgTCTGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGa                   >  1:61370/1‑140 (MQ=255)
                                                                                                         tGGCGCAATTCGCGCGGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTaa               <  2:551191/140‑1 (MQ=255)
                                                                                                                  tCGCGCGGTAAAATGAGTGCCCCAACCTTTGGTTGGTTCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCa      <  1:229694/140‑1 (MQ=255)
                                                                                                                      gcgGTAAAATGCGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGGCATCATGTACAAGTTAAGGAGGAAACCGTACCATCTg  >  1:257941/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                
AGGTACAGTGTTGTGGCGTTTCACGCGCCGGGTCATTTGTGGCCCCACCCAGCGTAATTGCACTTCGCGGGAGAAAAATCCACTTAGCCATTTGTTGATCGCGTCTGGCGCAATTCGCGCGGTAAAATGTGTGCCCCAAACTTCGGTTGGTGCGTCTTGTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTG  >  NC_000913/1007187‑1007444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑