| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 101,339 | T→C | V192A (GTC→GCC) | murC → | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 101,339 | 0 | T | C | 93.3% | 88.7 / ‑4.0 | 30 | V192A (GTC→GCC) | murC | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (12/16); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
AACGGCGGGCTGGTAAAAGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGA > NC_000913/101218‑101470 | aaCGGCGGGCTGGTAAAAGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGa < 1:758555/140‑1 (MQ=255) gggCTGGTAAAAGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACAt < 1:28522/140‑1 (MQ=255) gggCTGGTAAAAGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACAt < 1:787478/140‑1 (MQ=255) gggCTGGTAAAAGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACAt < 1:565864/140‑1 (MQ=255) aaGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGAtacctac < 1:712294/140‑1 (MQ=255) cggGGGGTCATGCGCGGTTGGGGGATGGTCGGTACCCGCTTGCCGAAGCAGATGAGAATGATGCATCGTTCCTGCATCTTCAACCCATGGTGGCGATTGGCACCAATATCCAAGCCGACCACATGGATACCCACCCgggg > 2:41774/1‑139 (MQ=255) cgTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAttt > 2:548106/1‑140 (MQ=255) aTGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACt < 1:533642/140‑1 (MQ=255) tGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACtt > 2:101595/1‑140 (MQ=255) gATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTc > 2:809813/1‑140 (MQ=255) aTTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCt > 2:704981/1‑140 (MQ=255) aGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGc < 1:548106/140‑1 (MQ=255) tGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACg > 1:603399/1‑140 (MQ=255) tGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTc > 1:16087/1‑140 (MQ=255) tCGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGTACAACCTGCCGTTTTACGGTCGTGc < 1:809813/140‑1 (MQ=255) cGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTc > 1:723415/1‑135 (MQ=255) cGTTCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTc < 2:723415/135‑1 (MQ=255) ttCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTGATTTTCTACACTACGTGCCGTGTTACGGTCGTGCGGt > 1:384808/1‑140 (MQ=255) ttCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGt < 1:101595/140‑1 (MQ=255) ttCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGt > 2:453221/1‑140 (MQ=255) tCCTGCATTTGCAACCGTTGGTCGCGATTGTCAACAATACCGAAGCCGGCCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTg < 2:384808/140‑1 (MQ=255) tCCTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTg < 1:453221/140‑1 (MQ=255) cTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGAt < 1:704981/140‑1 (MQ=255) cTGCATCTGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGAt < 2:16087/140‑1 (MQ=255) tGCAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGACGTGTTGCGGTCGCGCGGTGATGTGTGtt > 1:375327/1‑140 (MQ=255) cAACCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTtga < 1:63704/140‑1 (MQ=255) aaCCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGAtgt < 2:43115/132‑1 (MQ=255) aaCCGATGGTGGCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGAtgt > 1:43115/1‑132 (MQ=255) tggtggCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTTCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCg > 2:328199/1‑140 (MQ=255) ggCGATTGCCACCAATATCGAAGCCGACCAGATCGTTACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGa < 2:375327/140‑1 (MQ=255) ggCGATTGCCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGa > 2:608699/1‑140 (MQ=255) | AACGGCGGGCTGGTAAAAGCGGCGGGGGTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGA > NC_000913/101218‑101470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |