Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,020,576	A→G	V115A (GTG→GCG)	sulA ←	cell division inhibitor SulA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,020,576	0	A	G	100.0%	185.2 / NA	53	V115A (GTG→GCG)	sulA	cell division inhibitor SulA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/30);  total (23/30)

CGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGACTCAGTTTTTGTTG  >  NC_000913/1020442‑1020712                                                                                                                                       |                                                                                                                                         cGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCcaca                                                                                                                                     <  1:106774/140‑1 (MQ=255) cGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCcaca                                                                                                                                     <  2:662835/140‑1 (MQ=255) cGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCcaca                                                                                                                                     <  1:455599/140‑1 (MQ=255)   gAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAACTCAGCTGCCAACCAACCGATCGCCACACt                                                                                                                                   >  1:357368/1‑140 (MQ=255)     aaGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGt                                                                                                                                 <  2:737289/140‑1 (MQ=255)            ctcGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGcc                                                                                                                          >  2:549608/1‑140 (MQ=255)                tGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTg                                                                                                                      >  1:85767/1‑140 (MQ=255)                          ggATGCGCTTACCGGACACATACTAAACCCCATTGTGTTCCCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAgcgc                                                                                                            <  2:371987/140‑1 (MQ=255)                           gATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGcgcg                                                                                                           <  1:675683/140‑1 (MQ=255)                               cgcTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAAcc                                                                                                       <  1:648735/140‑1 (MQ=255)                                 cTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCAt                                                                                                     <  2:67116/140‑1 (MQ=255)                                    aCCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGa                                                                                                  >  1:644893/1‑140 (MQ=255)                                        gACGCATAATAAACCCCATAGCTTTCCCTTCATTTGCGGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTcc                                                                                              <  1:259719/140‑1 (MQ=255)                                         cagCATAATAAACCCTATAGCGTTAGCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             <  1:364545/138‑1 (MQ=255)                                         aCGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             <  1:286982/140‑1 (MQ=255)                                         aCGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             <  2:488934/140‑1 (MQ=255)                                         aCGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             <  2:51256/140‑1 (MQ=255)                                         aCGCATAATAAACCCCATAGCGATACCTTCATTTGCCCCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             <  1:245979/140‑1 (MQ=255)                                          cGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGAGCGCCACACGGTAGTTGCCCGTGCGTAAAGCGCGAACCATTGACTCcac                                                                                            >  2:259719/1‑140 (MQ=255)                                           gCATAATAAACCCCATAGCGTTGCCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCcaca                                                                                           <  1:400063/140‑1 (MQ=255)                                                  aaaCCCCATAGCGTTGCCTTCATTTACCGCATCAACAGGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGc                                                                                    <  1:401142/140‑1 (MQ=255)                                                       ccATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTTAGGAGCGAACCAATGACTCCACAGTGTGGCaactg                                                                               >  2:364545/1‑137 (MQ=255)                                                        cATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAggg                                                                               >  1:464717/1‑139 (MQ=255)                                                        cATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAggg                                                                               <  2:464717/139‑1 (MQ=255)                                                         aTAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             <  1:98084/124‑1 (MQ=255)                                                         aTAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCca                                                                                             >  2:98084/1‑124 (MQ=255)                                                         aTAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGga                                                                             >  2:810916/1‑140 (MQ=255)                                                         aTAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGga                                                                             >  2:150527/1‑140 (MQ=255)                                                         aTAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGga                                                                             >  2:196734/1‑140 (MQ=255)                                                          tAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGgag                                                                            >  2:496018/1‑140 (MQ=255)                                                           aGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGgaga                                                                           <  2:793317/140‑1 (MQ=255)                                                             cGTTACCTTCATTTGCCGCATCCACAAGTTCGGCATGCTGTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGc                                                                         <  2:357368/140‑1 (MQ=255)                                                                tACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTgg                                                                      >  2:191380/1‑140 (MQ=255)                                                                tACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTgg                                                                      >  1:604503/1‑140 (MQ=255)                                                                   cTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTa                                                                   <  1:549608/140‑1 (MQ=255)                                                                    ttCATTTGCCGCATCCACAAGTACACCATCCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTaa                                                                  <  1:268713/140‑1 (MQ=255)                                                                       aTTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCt                                                               >  1:655141/1‑140 (MQ=255)                                                                         ttGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGc                                                             >  2:705755/1‑140 (MQ=255)                                                                          tGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCa                                                            <  2:85767/140‑1 (MQ=255)                                                                           gCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCAt                                                           <  1:810916/140‑1 (MQ=255)                                                                           gCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCAt                                                           <  1:196734/140‑1 (MQ=255)                                                                            ccGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCt                                                                        <  2:582406/126‑1 (MQ=255)                                                                            ccGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCt                                                                        >  1:582406/1‑126 (MQ=255)                                                                               cATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACt                                                       <  1:191380/140‑1 (MQ=255)                                                                                     caaGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGtt                                                 <  2:644893/140‑1 (MQ=255)                                                                                                tGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGccc                                      >  2:534336/1‑140 (MQ=255)                                                                                                        ttcAGTCAAATCATCTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCt                              >  2:125205/1‑140 (MQ=255)                                                                                                                 atcatcTGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTAGTGTCGTTAAGGGAAGCCCAGATGCCTGAACCCAtt                     >  2:370685/1‑140 (MQ=255)                                                                                                                       tGCCAACCAACCGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGAc               >  2:476659/1‑140 (MQ=255)                                                                                                                            accaaccGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGACTCAGt          >  2:207073/1‑140 (MQ=255)                                                                                                                                accGATCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGACTCAGttttt      >  1:601239/1‑140 (MQ=255)                                                                                                                                    aTCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGACTCAGTTTttgttg  <  2:77786/140‑1 (MQ=255)                                                                                                                                    aTCGCCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGACTCAGTTTttgttg  <  1:705755/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCACCACACTGTAATTGCCCGTGCGTAAAGCGCGAACCATTGACTCCACAGTGTGGCAAGGGGAGAGCTGGCTAATCTGCATTACTTTCGTTAAGGGTAGCCCAGATGCCTGAACCCATTCCCGACTCAGTTTTTGTTG  >  NC_000913/1020442‑1020712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑