Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,076,112	C→T	C924Y (TGC→TAC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,076,112	0	C	T	100.0%	153.8 / NA	48	C924Y (TGC→TAC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/26);  total (22/26)

TCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGA  >  NC_000913/1075977‑1076241                                                                                                                                        |                                                                                                                                  tCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAg                                                                                                                               >  1:127185/1‑140 (MQ=255) tCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAg                                                                                                                               >  1:153911/1‑140 (MQ=255) tCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCACTTTGAACCTGTGGTCGGCAATCTCATCTTGCAGGTACAg                                                                                                                               >  2:297602/1‑140 (MQ=255)       ttGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCAcgcg                                                                                                                         <  1:599151/140‑1 (MQ=255)          gCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAg                                                                                                                      >  1:110904/1‑140 (MQ=255)            tttCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAgcg                                                                                                                    <  2:110904/140‑1 (MQ=255)            tttCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAgcg                                                                                                                    <  2:127185/140‑1 (MQ=255)             ttcgcttcgcTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATGTCAGCTGGCAGGTGCAGCACGCGCAgcgc                                                                                                                   >  1:227922/1‑140 (MQ=255)             ttcgcttcgcTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAgcgc                                                                                                                   >  2:60099/1‑140 (MQ=255)               cgcttcgctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCg                                                                                                                 >  2:777325/1‑140 (MQ=255)                 cttcgctATCAATCACTTGACCGATAGCGGTTGTCAGGCTACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAGTCTCATCTTGCAGGTACAGCACGCGCAGCGCCGaa                                                                                                               <  2:284882/140‑1 (MQ=255)                 cttcgctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGaa                                                                                                               <  1:60099/140‑1 (MQ=255)                 cttcgctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGaa                                                                                                               <  2:153911/140‑1 (MQ=255)                      ctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACg                                                                                                          <  2:71368/140‑1 (MQ=255)                      ctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACg                                                                                                          <  1:645350/140‑1 (MQ=255)                      ctATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACg                                                                                                          <  1:719810/140‑1 (MQ=255)                           aatcaCTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGAc                                                                                                     <  1:756550/140‑1 (MQ=255)                                 tGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCAc                                                                                               <  2:713406/140‑1 (MQ=255)                                   gACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTg                                                                                             >  1:518400/1‑140 (MQ=255)                                   gACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTg                                                                                             >  2:120698/1‑140 (MQ=255)                                     ccGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTc                                                                                           >  1:582689/1‑140 (MQ=255)                                        atatCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCCCGCGCAGAGCCTAACAACGCTAACCCGCACTGTCGaa                                                                                        >  1:367175/1‑140 (MQ=255)                                            cGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCTCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGcc                                                                                    <  2:722293/140‑1 (MQ=255)                                             ggtggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCTCACTGTCGAACGCCg                                                                                   >  2:105165/1‑140 (MQ=255)                                             ggtggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCg                                                                                   >  2:145289/1‑140 (MQ=255)                                              gtggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGa                                                                                  >  1:180395/1‑140 (MQ=255)                                                ggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAgg                                                                                <  2:432653/140‑1 (MQ=255)                                                 gtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGc                                                                               >  2:774942/1‑140 (MQ=255)                                                   cAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCa                                                                             >  1:696550/1‑140 (MQ=255)                                                                         cGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                              <  2:613422/133‑1 (MQ=255)                                                                         cGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                              >  1:613422/1‑133 (MQ=255)                                                                            cATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGt                                                    >  1:794602/1‑140 (MQ=255)                                                                            cATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGt                                                    <  1:145289/140‑1 (MQ=255)                                                                            cATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCCGt                                                    <  2:518400/140‑1 (MQ=255)                                                                            cATTCGGCCATTGCGACGCGCAGCAATTTTAACGTGTAGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGt                                                    <  1:367840/140‑1 (MQ=255)                                                                             aTTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGt                                                    <  2:135431/139‑1 (MQ=255)                                                                             aTTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGt                                                    >  1:135431/1‑139 (MQ=255)                                                                               tCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAg                                                 >  2:438558/1‑140 (MQ=255)                                                                               tCGGCCATTGCGCCGCGAAGCATTTTCAACGCGTGATCGACAATCTCACCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAg                                                 <  2:227922/140‑1 (MQ=255)                                                                                 ggCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTg                                               <  2:180395/140‑1 (MQ=255)                                                                                         cgccgcGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTgaagaa                                       >  2:120158/1‑140 (MQ=255)                                                                                         cgccgcGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTgaagaa                                       >  2:757296/1‑140 (MQ=255)                                                                                              cgcAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGAc                                  >  1:726568/1‑140 (MQ=255)                                                                                                cagcaTTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCTCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACaa                                <  1:105165/140‑1 (MQ=255)                                                                                                    aTTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAatcat                            <  2:794602/140‑1 (MQ=255)                                                                                                                   tCGGCAATCTCATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgccgcc             <  1:774942/140‑1 (MQ=255)                                                                                                                             cATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCg   <  1:757296/140‑1 (MQ=255)                                                                                                                             cATCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCg   <  1:120158/140‑1 (MQ=255)                                                                                                                              aTCTTGCAGGTACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGa  <  2:726568/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  TCAATATTGGCTTTCGCTTCGCTATCAATCACTGGACCGATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGA  >  NC_000913/1075977‑1076241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑