Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,076,565	A→G	V773A (GTG→GCG)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,076,565	0	A	G	100.0%	92.8 / NA	27	V773A (GTG→GCG)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

ATGGCGATCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCAC  >  NC_000913/1076433‑1076698                                                                                                                                     |                                                                                                                                      aTGGCGATCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAgg                                                                                                                                >  1:2945/1‑140 (MQ=255)       aTCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTaa                                                                                                                          >  2:733442/1‑140 (MQ=255)              gcgcGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACgg                                                                                                                   <  1:733442/140‑1 (MQ=255)                      aTCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATgg                                                                                                                       <  1:621509/128‑1 (MQ=255)                      aTCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATgg                                                                                                                       >  2:621509/1‑128 (MQ=255)                         aGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGtt                                                                                                        >  1:786168/1‑140 (MQ=255)                           gggcgTTTTTTCTGCCTGTTTTTCCAGCACGTTTTTACCGGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAg                                                                                                      <  2:391922/139‑1 (MQ=255)                                      cTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATcc                                                                                           <  2:2945/140‑1 (MQ=255)                                        gCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCg                                                                                         >  2:453688/1‑140 (MQ=255)                                        gCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCg                                                                                         >  2:653927/1‑140 (MQ=255)                                         ccGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGc                                                                                        <  2:42592/140‑1 (MQ=255)                                                tGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTc                                                                                 <  1:453688/140‑1 (MQ=255)                                                       aCGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCgta                                                                          >  1:477624/1‑140 (MQ=255)                                                       aCGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCgta                                                                          <  1:588317/140‑1 (MQ=255)                                                                  tGCCGCCAGTGCGGCGGCGATCTGCCCGGGGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCGCAGGCCCTAATGGACGGTGGGGTTCGTTAGCGAAATCATCCCGCACCTGTCCGCCGTAGCAGTGGAGaa                                                               >  1:805989/1‑140 (MQ=255)                                                                     cgccAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAAt                                                            >  1:32314/1‑140 (MQ=255)                                                                       ccAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCTCCTATCTGGCGTACTAGTGGAGAAAATCg                                                          >  1:376626/1‑140 (MQ=255)                                                                                  gcgATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGTCTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTcgcg                                               <  1:653927/140‑1 (MQ=255)                                                                                  gcgATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTcgcg                                               <  2:32314/140‑1 (MQ=255)                                                                                                        ccAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTg                         >  1:589762/1‑140 (MQ=255)                                                                                                         cAGCGGGAAGTTCCACGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGa                        >  2:744194/1‑140 (MQ=255)                                                                                                                       aCGGACTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGcc          <  2:589762/140‑1 (MQ=255)                                                                                                                          gACTGATACACGCCACTAGCCCTAATGGACCGTAGGTTTCGTTAGCGAGATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCa       <  2:394616/140‑1 (MQ=255)                                                                                                                            ttGATACGCGCCTCAGGCACTAATCGGCGGTGGGTTTCGTTAGCGACATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCCTCGCGCCATTCGGAAATGGCGTTACTGAAAGATTTTCCGGCCTCACg     <  2:805989/139‑1 (MQ=255)                                                                                                                            cTGATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACg     <  2:786168/140‑1 (MQ=255)                                                                                                                              gATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACg     >  2:522247/1‑138 (MQ=255)                                                                                                                              gATACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACg     <  1:522247/138‑1 (MQ=255)                                                                                                                               aTACACGCCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCAc  <  2:477624/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      ATGGCGATCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCAC  >  NC_000913/1076433‑1076698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑