Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,099,236	A→G	T120A (ACT→GCT)	ycdX →	zinc‑binding phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,099,236	0	A	G	100.0%	176.6 / NA	50	T120A (ACT→GCT)	ycdX	zinc‑binding phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

AACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACT  >  NC_000913/1099101‑1099371                                                                                                                                        |                                                                                                                                        aaCATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATCACAAAGCAACCCATACACAAGCGATGATCGCCGCTAt                                                                                                                                     >  1:395635/1‑140 (MQ=255)           aTGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGc                                                                                                                           <  1:46162/140‑1 (MQ=255)            tGTTGATGGTGAAATTGTCTGCAGCGGTAAAATGTTTGAATCGCTGGACCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCa                                                                                                                          <  2:254046/140‑1 (MQ=255)            tGTTGATGGTGAAATTGACTGCAGCCGTAAAATGTTTCACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCa                                                                                                                          <  1:276062/140‑1 (MQ=255)             gTTGATGGTGAAATTGATTGCAACGGTAAAATGTTTGACTCGCTGGATTTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCaa                                                                                                                         <  2:329861/140‑1 (MQ=255)             gTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCaa                                                                                                                         >  2:803642/1‑140 (MQ=255)             gTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCaa                                                                                                                         >  2:784842/1‑140 (MQ=255)              ttGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAAt                                                                                                                        >  1:103782/1‑140 (MQ=255)              ttGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAAt                                                                                                                        <  2:103782/140‑1 (MQ=255)              ttGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAAt                                                                                                                        <  1:445053/140‑1 (MQ=255)              ttGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAAt                                                                                                                        <  1:788529/140‑1 (MQ=255)              ttGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAAt                                                                                                                        <  1:772663/140‑1 (MQ=255)              ttGATGGTGAAATTGACTGCAGCGGTAAAATCTTTAACTAGCTGGATCGAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAAt                                                                                                                        <  2:238778/140‑1 (MQ=255)                  tGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGc                                                                                                                    <  2:124698/140‑1 (MQ=255)                  tGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGc                                                                                                                    <  1:416790/140‑1 (MQ=255)                   ggTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   <  2:418806/140‑1 (MQ=255)                   ggTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   <  2:178315/140‑1 (MQ=255)                   ggTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   <  1:102008/140‑1 (MQ=255)                   ggTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   <  1:661054/140‑1 (MQ=255)                   ggTGAAATTGACTGCAGAGGTAAAATGTTTGACTCACTGAATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   <  2:265749/140‑1 (MQ=255)                       aaaTTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCACCGATATCGCCAGAGGCAATGTGCAtata                                                                                                               >  1:265749/1‑140 (MQ=255)                           tGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   <  1:498488/132‑1 (MQ=255)                           tGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAACGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCa                                                                                                                   >  2:498488/1‑132 (MQ=255)                                   gCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATccc                                                                                                   >  1:205675/1‑140 (MQ=255)                                     ggTAAAATGTTTGACTCGCTGGGTCTAATTATTGCCGGTCTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCgg                                                                                                 <  2:261191/140‑1 (MQ=255)                                     ggTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCgg                                                                                                 <  2:664238/140‑1 (MQ=255)                                       tAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGaa                                                                                               <  1:350864/140‑1 (MQ=255)                                             gTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCa                                                                                         <  1:803642/140‑1 (MQ=255)                                                tGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTTTCGCCAGCCGCAGTGTGCATATAATAAGCCTTCCCGGAAATCCCAAat                                                                                      >  2:227189/1‑140 (MQ=255)                                                   cTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATg                                                                                   >  2:558198/1‑140 (MQ=255)                                                         ggATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAg                                                                             <  1:551587/140‑1 (MQ=255)                                                                    ttGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGcc                                                                  <  1:784842/140‑1 (MQ=255)                                                                     tGCCGGTTTTCATGAGCCGGTTTTTGCACCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCg                                                                 >  2:792626/1‑140 (MQ=255)                                                                      gCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGt                                                                >  1:613671/1‑140 (MQ=255)                                                                      gCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGt                                                                >  2:128376/1‑140 (MQ=255)                                                                      gCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGt                                                                >  2:101964/1‑140 (MQ=255)                                                                                    gCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCcg                                                  >  1:691589/1‑140 (MQ=255)                                                                                     ccGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCcgc                                                 <  2:110074/140‑1 (MQ=255)                                                                                     ccGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCcgc                                                 >  1:110074/1‑140 (MQ=255)                                                                                         tttttGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGACGTGAAAGCCGTTGCTGAAGCAGCCGCGaaa                                             <  2:205675/140‑1 (MQ=255)                                                                                                                 ccAATACACAAGCGATGATCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCaat                     >  1:691961/1‑140 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGGAGCCGCGAGACAGCAGTTGGCGCTGGAAATGAATAATTCCTCAttttt       >  2:218328/1‑140 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAttttt       >  2:65065/1‑140 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAttttt       >  2:644479/1‑140 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAttttt       <  1:107927/140‑1 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAttttt       >  2:186523/1‑140 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCAttttt       >  1:484103/1‑140 (MQ=255)                                                                                                                               atgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGAAGCCGCGATACAACCGGCGGCGCTGTAAATCTATAATTCCTCAttttt       >  2:401298/1‑140 (MQ=255)                                                                                                                                tgatCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTa      <  1:65065/140‑1 (MQ=255)                                                                                                                                  atCGCCGCTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTaca    <  2:613671/140‑1 (MQ=255)                                                                                                                                    cgccgcTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt  <  1:128376/140‑1 (MQ=255)                                                                                                                                    cgccgcTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt  <  2:691961/140‑1 (MQ=255)                                                                                                                                    cgccgcTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt  <  2:769454/140‑1 (MQ=255)                                                                                                                                    cgccgcTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt  >  1:439425/1‑140 (MQ=255)                                                                                                                                    cgccgcTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt  <  1:101964/140‑1 (MQ=255)                                                                                                                                    cgccgcTATCGCCAGCGGCAATGTGCATACAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACt  <  1:227189/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AACATTAAAAATGTTGATGGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTTCATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACTATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACT  >  NC_000913/1099101‑1099371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑