Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,133,166	T→C	V198A (GTC→GCC)	flgE →	flagellar hook protein FlgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,133,166	0	T	C	100.0%	150.1 / NA	44	V198A (GTC→GCC)	flgE	flagellar hook protein FlgE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

GATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGA  >  NC_000913/1133038‑1133300                                                                                                                                 |                                                                                                                                       gATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACGGTCAGGGAAATGCTCATGACAGGAGCGCCTACTTTGTGa                                                                                                                             >  2:325802/1‑140 (MQ=255)    cAACCTGAATTCCAGAGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGa                                                                                                                          <  1:325802/140‑1 (MQ=255)     aaCCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGAc                                                                                                                         <  1:592361/140‑1 (MQ=255)       ccTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCg                                                                                                                       >  2:496838/1‑140 (MQ=255)       ccTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCg                                                                                                                       >  2:453811/1‑140 (MQ=255)       ccTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCg                                                                                                                       <  1:453811/140‑1 (MQ=255)       ccTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGCCAGGGCAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCg                                                                                                                       >  1:734657/1‑140 (MQ=255)                     aTCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGTATAATAACTgg                                                                                                         <  2:734657/140‑1 (MQ=255)                      tCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGc                                                                                                        <  1:660927/140‑1 (MQ=255)                          cTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGt                                                                                                    <  1:111110/140‑1 (MQ=255)                               tACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTaca                                                                                               >  2:781057/1‑140 (MQ=255)                               tACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTaca                                                                                               >  2:147980/1‑140 (MQ=255)                                 cTGTTACGCCATTCAGCGCCAGCAATGCGCATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACAcc                                                                                             <  1:302679/140‑1 (MQ=255)                                 cTGTTACGCCATTCACCGCCAGTAACGCGCATAGCTACAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACAcc                                                                                             <  1:217042/140‑1 (MQ=255)                                    ttACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAg                                                                                          >  1:524037/1‑140 (MQ=255)                                     tACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAgg                                                                                         >  2:153635/1‑140 (MQ=255)                                       cGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACAcc                                                                                             <  2:97565/134‑1 (MQ=255)                                       cGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACAcc                                                                                             >  1:97565/1‑134 (MQ=255)                                       cGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGAt                                                                                       >  1:532932/1‑140 (MQ=255)                                                 cgcCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGGAGACCGGGGATATTAACTGGCAGGTGTACAGCCAGGATAGCAGTGATc                                                                             >  2:241452/1‑140 (MQ=255)                                                     aGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCaaa                                                                         <  2:532932/140‑1 (MQ=255)                                                      gCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACAGGGGATAACAACAGGCAGGTCTACAGCCAGTATAGCAGTGATCCAAAc                                                                        >  2:243732/1‑140 (MQ=255)                                                       cAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACa                                                                       >  1:41045/1‑140 (MQ=255)                                                                  gCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAg                                                            <  1:443651/140‑1 (MQ=255)                                                                    tataACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGAc                                                          <  1:496838/140‑1 (MQ=255)                                                                    tataACAAAAAAGGTTCGGTGACTGATTTGGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGAc                                                          <  1:243732/140‑1 (MQ=255)                                                                          aaaaaaGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGac                                                    <  2:424445/140‑1 (MQ=255)                                                                            aaaaGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGacaa                                                  <  2:41045/140‑1 (MQ=255)                                                                                 gTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTg                                             <  2:524037/140‑1 (MQ=255)                                                                                 gTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTg                                             <  1:781057/140‑1 (MQ=255)                                                                                 gTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTg                                             <  1:147980/140‑1 (MQ=255)                                                                                      gTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTgg                                            <  2:99997/136‑1 (MQ=255)                                                                                      gTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTgg                                            >  1:99997/1‑136 (MQ=255)                                                                                           tGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATg                                   >  2:794372/1‑140 (MQ=255)                                                                                                 cGACAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATGCAAACAGCATTGCGAAGACAGCGACCACACTGGAATTTAATGCTAATg                             >  2:371399/1‑140 (MQ=255)                                                                                                   aCAGTCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGc                           <  1:153635/140‑1 (MQ=255)                                                                                                       tCAGGGTAATGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACAt                       >  1:590696/1‑140 (MQ=255)                                                                                                              aaTGCTCATGACATGAGCGCCTACTTTGTGAAGACCGGGCATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGa                <  2:314842/140‑1 (MQ=255)                                                                                                                   tCATGACATGAGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTg           >  1:413289/1‑140 (MQ=255)                                                                                                                     atgacatgaGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCg         >  1:538653/1‑140 (MQ=255)                                                                                                                          atgaGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATg      >  1:813990/1‑138 (MQ=255)                                                                                                                          atgaGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATg      <  2:813990/138‑1 (MQ=255)                                                                                                                            gaGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATTCAAACAGCATTGCGAAGACAGCGACATCACTGGAATTTAATGCAAATGGCACTTTAGGGGATGGTGCGATGGCGa  >  2:315276/1‑140 (MQ=255)                                                                                                                            gaGCGCCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGa  >  2:104186/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       GATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGA  >  NC_000913/1133038‑1133300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑