Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,750	A→G	V45A (GTT→GCT)	yadN ←	putative fimbrial protein YadN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,750	0	A	G	100.0%	174.1 / NA	50	V45A (GTT→GCT)	yadN	putative fimbrial protein YadN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/30);  total (20/30)

CTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAA  >  NC_000913/156624‑156886                                                                                                                               |                                                                                                                                         ctgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTcaca                                                                                                                             >  1:565659/1‑140 (MQ=255)      gTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGgtgt                                                                                                                        <  1:722058/140‑1 (MQ=255)       tCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGACAGCCCGGGTTTCACAGGTGtt                                                                                                                       >  2:310421/1‑140 (MQ=255)         aaCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTAt                                                                                                                     >  1:153389/1‑140 (MQ=255)          aCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATc                                                                                                                    <  1:739228/140‑1 (MQ=255)          aCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATc                                                                                                                    <  1:700985/140‑1 (MQ=255)             gTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATcaac                                                                                                                 <  2:18393/140‑1 (MQ=255)                aTGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCaacaac                                                                                                              <  1:449735/140‑1 (MQ=255)                aTGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCaacaac                                                                                                              >  1:709181/1‑140 (MQ=255)                 tGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAAcc                                                                                                             >  2:149668/1‑140 (MQ=255)                 tGCTGAAAGGTTTAGCCTTAGCGCCAACGGGGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGGTTCACAGGTGTTATCAACAAcc                                                                                                             >  2:484385/1‑140 (MQ=255)                  gCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCa                                                                                                            <  2:153389/140‑1 (MQ=255)                  gCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCa                                                                                                            <  2:709181/140‑1 (MQ=255)                  gCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATGCAGCACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCa                                                                                                            <  2:335389/140‑1 (MQ=255)                   cTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAg                                                                                                           <  1:654820/140‑1 (MQ=255)                        aGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                                                      <  2:678384/140‑1 (MQ=255)                        aGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                                                      <  2:637282/140‑1 (MQ=255)                        aGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                                                      <  2:513754/140‑1 (MQ=255)                        aGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGCTACCGCCGCCAGCGAGGGTTTCACATGTGTTATCAACAACCAGACCAc                                                                                                      >  2:355577/1‑140 (MQ=255)                        aGGTTTAGCTTTAGCGCAAACGGTGTCATTCAGTACGCCCGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                                                      <  2:349650/140‑1 (MQ=255)                         ggTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACt                                                                                                     <  1:514334/140‑1 (MQ=255)                          gTTTAGCTTTAGCGCCAGCGGTGTCATTCAGGACGACAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGCTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTg                                                                                                    <  2:288847/140‑1 (MQ=255)                              agctttagcGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGatat                                                                                                >  1:15651/1‑140 (MQ=255)                               gctttagcGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATAtt                                                                                               <  1:551903/140‑1 (MQ=255)                                     gcgcCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTg                                                                                         >  1:760174/1‑140 (MQ=255)                                         cAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAccg                                                                                     <  2:663560/140‑1 (MQ=255)                                         cAACGGTGACATTCAGTACGCCCGCATCGATTTTCTCAGTGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAccg                                                                                     <  2:363235/140‑1 (MQ=255)                                          aaCGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGat                                                                                                  >  2:507882/1‑126 (MQ=255)                                          aaCGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGat                                                                                                  <  1:507882/126‑1 (MQ=255)                                                         tACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATcagcag                                                                     >  1:110856/1‑140 (MQ=255)                                                         tACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATcagcag                                                                     >  1:484878/1‑140 (MQ=255)                                                          aCGCCAGCATCGATTCCACCAACGGTTGCAGTTTGCACCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGa                                                                    <  1:221768/140‑1 (MQ=255)                                                               aGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAg                                                               <  2:110856/140‑1 (MQ=255)                                                                 cATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTa                                                             >  2:458379/1‑140 (MQ=255)                                                                          cACCAACGGTTGCGGTTTGCAGGAAGATCAGGCCATCCTTGTTACCGCGGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAAcc                                                    <  2:360045/140‑1 (MQ=255)                                                                          cACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAAcc                                                    <  2:654049/140‑1 (MQ=255)                                                                               aCGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGc                                               <  2:522268/140‑1 (MQ=255)                                                                               aCGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGc                                               <  1:39276/140‑1 (MQ=255)                                                                                cGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGcc                                              <  2:812887/140‑1 (MQ=255)                                                                                     gCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTGCATGTAAGCAGAAGCAGTACCTCCAACCATCGCCAGCAt                                         >  2:363476/1‑140 (MQ=255)                                                                                     gCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCAt                                         >  1:177347/1‑140 (MQ=255)                                                                                             cagcagGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAg                                 >  1:97906/1‑140 (MQ=255)                                                                                                cagGATCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACa                              >  1:29136/1‑140 (MQ=255)                                                                                                   gATCAGACCATCCTTTTTACCGTCGCCAGTGCGGGTTGCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAggg                           <  1:310421/140‑1 (MQ=255)                                                                                                     tCAGACCATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCa                         >  2:536500/1‑140 (MQ=255)                                                                                                           cATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGAc                               >  2:450687/1‑128 (MQ=255)                                                                                                           cATCCTTGTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGAc                               <  1:450687/128‑1 (MQ=255)                                                                                                                  gTTACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCAAAACCTAAttttt            >  2:420731/1‑140 (MQ=255)                                                                                                                   ttACCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCAAAACCTAAtttttt           >  2:724811/1‑140 (MQ=255)                                                                                                                     aCCGCCGTCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAg         <  2:760174/140‑1 (MQ=255)                                                                                                                            tCAGCGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATaaa  <  1:413059/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         CTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAA  >  NC_000913/156624‑156886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑