Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,172,696	A→G	R423R (CGT→CGC)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,172,696	0	A	G	100.0%	90.1 / NA	27	R423R (CGT→CGC)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/17);  total (10/17)

GGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCGGACCGTCGAAAGTCTCGA  >  NC_000913/1172565‑1172832                                                                                                                                    |                                                                                                                                         gggTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACg                                                                                                                                  <  2:829368/140‑1 (MQ=255)    ttGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTc                                                                                                                               >  1:782057/1‑140 (MQ=255)     tGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCg                                                                                                                              <  2:782057/140‑1 (MQ=255)      gATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGc                                                                                                                             >  1:91334/1‑140 (MQ=255)          gTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGc                                                                                                                         <  2:91334/140‑1 (MQ=255)           tGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCt                                                                                                                        >  1:683624/1‑140 (MQ=255)            gcggcgAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCtt                                                                                                                       >  1:722887/1‑140 (MQ=255)             cggcgAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATGATCGGACAGCGCCCACGGTCGCTGGCTtc                                                                                                                      >  1:301601/1‑140 (MQ=255)                cgAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTtcatc                                                                                                                   <  1:139461/140‑1 (MQ=255)                 gagaATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTtcatca                                                                                                                  >  2:760415/1‑140 (MQ=255)                          gacgacgacGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATa                                                                                                         <  2:683624/140‑1 (MQ=255)                                gacgGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGt                                                                                                   >  1:678794/1‑140 (MQ=255)                                       aaCGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAg                                                                                            <  2:95014/140‑1 (MQ=255)                                       aaCGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAg                                                                                            <  2:818995/140‑1 (MQ=255)                                                      cAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTg                                                                             >  2:89640/1‑140 (MQ=255)                                                        gATCGCTTACGCAAGTCGGCGGAAGATTACGAAGCATATCGACAAAACAACCTGCGGCAGCGCCAAACAGCAGATAGCGCCCACAGTCGCTGGCTTCATCTAGACGGATAATGCGTTGCGGAGCAATTTTAATGCCTGCg                                                                           <  2:372277/140‑1 (MQ=255)                                                         aTCGCTTTCGCAAATCAGCGCCGGATTACGCACCGGATCGACAAAACCTTGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGa                                                                          <  2:289444/140‑1 (MQ=255)                                                         aTCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGa                                                                          <  2:827864/140‑1 (MQ=255)                                                         aTCGCTTTCGCAAATCACCGCCAGATTACGCCCCGCATCAACAAAACCGTGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGa                                                                          <  2:228808/140‑1 (MQ=255)                                                                                          cGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCGCCGTTTCACGGCAACCTTCAc                                         >  1:750363/1‑140 (MQ=255)                                                                                           gTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACt                                        <  2:678794/140‑1 (MQ=255)                                                                                                       ccATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGaa                            <  2:436316/140‑1 (MQ=255)                                                                                                       ccATGTTCGGCAGCGCCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGaa                            <  1:96509/140‑1 (MQ=255)                                                                                                               ggCCGCGGCAATCATCAGATAGCGCCCCCGGTCGCTGGCTTTCTCAAGTCGGCTAATTCGGTGGGGAGGAATTTTAATTCGTGCGAACAGTTTCCCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCg                    <  2:750363/140‑1 (MQ=255)                                                                                                                 cAGCGCCAATCATCAGATAGCGCCCACGGTCCCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCGGa                  <  2:301601/140‑1 (MQ=255)                                                                                                                     gcCAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCGGACCGt              <  2:722887/140‑1 (MQ=255)                                                                                                                      ccAATCATCAGATAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTACACTTTCTGCCGAAAACACCACCGGACCGTc             >  2:231513/1‑140 (MQ=255)                                                                                                                                 aTAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCGGACCGTCGAAAGTCTCGa  <  2:47457/140‑1 (MQ=255)                                                                                                                                 aTAGCGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCGGACCGTCGAAAGTCTCGa  <  1:87052/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         GGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTCTACCGAGAACACCACCGGACCGTCGAAAGTCTCGA  >  NC_000913/1172565‑1172832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑