Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,367	A→G	W155R (TGG→CGG)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,367	0	A	G	100.0%	176.3 / NA	51	W155R (TGG→CGG)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/22);  total (29/22)

GCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCACAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGA  >  NC_000913/1182235‑1182501                                                                                                                                     |                                                                                                                                       gCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCg                                                                                                                                 >  2:207747/1‑140 (MQ=255)             cAGCTTCAATCTCTTTCGGATCGGTGGTGTTACTGTAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTg                                                                                                                     <  2:350419/140‑1 (MQ=255)              aGCTTCAATCTCTTTTGGATCGCTGTTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGa                                                                                                                    <  1:261662/140‑1 (MQ=255)               gCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGAc                                                                                                                   >  2:775754/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  2:633080/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  2:199748/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  1:142861/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  2:500955/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  2:806008/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  1:458181/1‑140 (MQ=255)                cTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTACCGCAGATCGGCCCAGCTGGTGACa                                                                                                                  >  1:314391/1‑140 (MQ=255)                   cAATCTCTTTCGGATCGTTGGTATTACCGGAGTAGCCCATCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGAt                                                                                                               <  2:314391/140‑1 (MQ=255)                   cAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGcc                                                                                                                              >  1:632248/1‑125 (MQ=255)                   cAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGcc                                                                                                                              <  2:632248/125‑1 (MQ=255)                     aTCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGAttt                                                                                                             >  2:782522/1‑140 (MQ=255)                          tttCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGAt                                                                                                        >  1:105132/1‑140 (MQ=255)                                    gtggtgTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATc                                                                                              >  2:513105/1‑140 (MQ=255)                                    gtggtgTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATc                                                                                              >  1:670558/1‑140 (MQ=255)                                    gtggtgTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATc                                                                                              <  2:778130/140‑1 (MQ=255)                                            aCCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTaa                                                                                      >  1:810293/1‑140 (MQ=255)                                                   gtagccAGCTTACGCAGCGCCAGCTTGAAAACTTCACCGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTTGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAAt                                                                               <  1:286572/136‑1 (MQ=255)                                                     gCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCg                                                                             <  1:775754/140‑1 (MQ=255)                                                     gCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCg                                                                             >  2:596475/1‑140 (MQ=255)                                                         aGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGt                                                                         <  2:698477/140‑1 (MQ=255)                                                           cTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCg                                                                       >  1:9399/1‑140 (MQ=255)                                                           cTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCg                                                                       >  2:447347/1‑140 (MQ=255)                                                                gCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCAccc                                                                  <  1:596475/140‑1 (MQ=255)                                                                gCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCAccc                                                                  <  1:199748/140‑1 (MQ=255)                                                                   gcgcCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAg                                                               <  2:142861/140‑1 (MQ=255)                                                                    cgcCTTCTGGAACACTTCACCGCAATCGTCGGCCAACAGAAGGCTGCCTTTGTACTCTGGCTTCAGCAGATCGGCACAGCTGGGGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGa                                                              >  2:130887/1‑140 (MQ=255)                                                                       cATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGatat                                                           <  2:810030/140‑1 (MQ=255)                                                                        aTCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGAtata                                                          <  2:458181/140‑1 (MQ=255)                                                                        aTCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGAtata                                                          <  2:810293/140‑1 (MQ=255)                                                                        aTCTGGAACACTTCACGGGCATAGTCGGTCAACAGCAGGTTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGAtata                                                          <  2:322850/140‑1 (MQ=255)                                                                                   ttCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCAGCACCGTTAGCACAAATACCCGTCGCGCCCCATATATACGAAATGGagt                                               >  2:385324/1‑140 (MQ=255)                                                                                          gCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGtt                                        >  1:119291/1‑140 (MQ=255)                                                                                             tcgtcgGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTAtt                                     >  2:436479/1‑140 (MQ=255)                                                                                             tcgtcgGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTAtt                                     >  2:565769/1‑140 (MQ=255)                                                                                             tcgtcgGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCATCGCACCTCAGGTAGACGGAACGGAGTAGTCGTTAtt                                     >  1:256066/1‑140 (MQ=255)                                                                                              cgtcgGTCAACAGCATGCTGCCTTTATACCCTGGCTTGCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTc                                    <  1:232505/140‑1 (MQ=255)                                                                                                    tCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATGGCCGTCGCGCCCTAGAAATACGGAAGGGAGAAGTCGTTATTCGGGTCa                              >  2:335108/1‑140 (MQ=255)                                                                                                       aCAGCAGGCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCaaaa                           <  1:89846/140‑1 (MQ=255)                                                                                                             ggCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCttg                     <  1:782522/140‑1 (MQ=255)                                                                                                              gCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCttgt                    >  2:37028/1‑140 (MQ=255)                                                                                                              gCTGCCTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCttgt                    <  2:670558/140‑1 (MQ=255)                                                                                                                  ccTTTGTACTCTGGCTTCCGCAGATTGGCGCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAg                <  1:335108/140‑1 (MQ=255)                                                                                                                  ccTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAg                <  2:9399/140‑1 (MQ=255)                                                                                                                  ccTTTGTACTCTGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAg                <  1:513105/140‑1 (MQ=255)                                                                                                                  ccTTTGTACTCTGGCTTACGCAGATCGGCCCCGCTGGTGACAGATTTCTGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAg                <  2:293341/140‑1 (MQ=255)                                                                                                                            ctGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGa      >  2:519682/1‑140 (MQ=255)                                                                                                                             tGGCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCTTTATTCGAGTCCAACGGCTTGATGAGCATGTCTGGAt     >  1:390974/1‑140 (MQ=255)                                                                                                                               gCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCg   >  1:546922/1‑140 (MQ=255)                                                                                                                               gCTTCCGCAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCg   >  1:529525/1‑140 (MQ=255)                                                                                                                                cTTCCGCAGTTCGGCCCCGCTGTTGACAGCTCTCGGATCAACCGCATCGCCGTTAACTCCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCga  <  2:256066/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GCTCGTTATATGCAGCTTCAATCTCTTTCGGATCGGTGGTGTTACCGGAGTAGCCCAGCTTACGCAGCGCCATCTGGAACACTTCACGGGCATCGTCGGTCAACAGCAGGCTGCCTTTGTACTCTGGCTTCCACAGATCGGCCCAGCTGGTGACAGATTTCGGATCCACCGCATCACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGA  >  NC_000913/1182235‑1182501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑