| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,184,982 | T→C | T205A (ACT→GCT) | potA ← | spermidine preferential ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,184,982 | 0 | T | C | 98.0% | 172.7 / ‑4.6 | 52 | T205A (ACT→GCT) | potA | spermidine preferential ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (22/28); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TCGCCAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGCTTGTTAACCACCG > NC_000913/1184846‑1185113 | tCGCCAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCcac > 2:785902/1‑140 (MQ=255) cGCCAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGTGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCcaca < 2:823842/140‑1 (MQ=255) ccAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTCAGTTCTTCTTCCTAGTCGTGAGCCACaaa > 1:396564/1‑140 (MQ=255) aGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAAt > 1:673846/1‑140 (MQ=255) ccGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGt < 2:58914/140‑1 (MQ=255) caacaaACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATg > 1:654672/1‑140 (MQ=255) aacaaacaGGTTTTTCTACTCTTCGTAGATTACGCGCGGTGTGCCGTCCTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGc < 2:402897/140‑1 (MQ=255) acaaacaGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGcc < 1:323228/140‑1 (MQ=255) ggTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTa < 1:452396/140‑1 (MQ=255) ggTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTa < 1:632493/140‑1 (MQ=255) tttCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCt > 1:192359/1‑140 (MQ=255) ggCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTaa < 2:704938/140‑1 (MQ=255) tctTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGc < 1:49867/140‑1 (MQ=255) ttCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCtt < 1:785902/140‑1 (MQ=255) gTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCa < 2:673846/140‑1 (MQ=255) aCGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCt > 1:445201/1‑140 (MQ=255) cggcgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCa < 1:40095/140‑1 (MQ=255) gcgTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCAtt > 2:106642/1‑140 (MQ=255) cTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGc < 2:445201/140‑1 (MQ=255) ttGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCa < 1:664919/140‑1 (MQ=255) cTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCATGCTTACGCCGTAACGCTTTCAGCTCATTCTGCATTTGCTTACGCAGtt > 2:331750/1‑140 (MQ=255) tGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTaa > 1:522719/1‑140 (MQ=255) tGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGAGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACg < 2:497194/130‑1 (MQ=255) tGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGAGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACg > 1:497194/1‑130 (MQ=255) cGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATc < 1:87530/140‑1 (MQ=255) acgaTCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCa > 2:524290/4‑140 (MQ=255) aCCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCa > 2:781140/1‑140 (MQ=255) ccATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCGGCTCGTTATGCTTTAGCTTACACAGTTTGTAATCCAg > 1:234272/1‑140 (MQ=255) ccATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAg > 2:96525/1‑140 (MQ=255) gcATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTgag > 1:35036/1‑140 (MQ=255) accacAATCCTGTCTGACATGGGGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGa < 2:396564/140‑1 (MQ=255) accacAATCCTGTCTGAAATGGCGAGTGCTTATTCCTGGCCGTGAGCCGCAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGa < 2:234272/140‑1 (MQ=255) aaTCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCAt < 2:35036/140‑1 (MQ=255) gACATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGcaacaa > 1:518687/1‑140 (MQ=255) cATGGTGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAacaaca > 2:679780/1‑140 (MQ=255) cATGGTGAGTGCTTCCTCCTGGTCGGGAGGCACAAAGCCGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAacaaca < 1:331750/140‑1 (MQ=255) tGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCa < 2:79429/126‑1 (MQ=255) tGAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCa > 1:79429/1‑126 (MQ=255) gAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAg < 2:522719/140‑1 (MQ=255) gAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAg < 1:679780/140‑1 (MQ=255) gAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAg < 2:557812/140‑1 (MQ=255) gAGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAg < 2:192359/140‑1 (MQ=255) aGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCATTTTGTAATACAGCGCTCTGAGCGACCCATCACGCAACAATAGACGAgg > 2:389272/1‑140 (MQ=255) aGTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAgg > 1:585745/1‑140 (MQ=255) gTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGc < 2:455897/140‑1 (MQ=255) gTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGc < 1:781140/140‑1 (MQ=255) gTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGc < 1:524290/140‑1 (MQ=255) gTGCTTCTTCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGc < 1:96525/140‑1 (MQ=255) cttcttCCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGCTTg > 2:750235/1‑140 (MQ=255) ttcttcCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGCTTGt < 1:106642/140‑1 (MQ=255) cttcCTGGTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATGCAACAACAATAGACGAGGCTTGTTa > 1:332458/1‑140 (MQ=255) gTCGTGAGCCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTAATCAAGCAACAAAAGACAAGGCTTGTTAACCACcg > 2:262749/1‑140 (MQ=255) | TCGCCAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCCTGTCTGACATGGTGAGTGCTTCTTCCTGGTCGTGAGTCACAAAGACGAATGTAATGCCAAGCTTACGCTGTAACGCTTTCAGCTCGTTCTGCATTTGCTTACGCAGTTTGTAATCCAGCGCTGAGAGCGACTCATCCAGCAACAACAGACGAGGCTTGTTAACCACCG > NC_000913/1184846‑1185113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |