Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,194,643	T→C	K103K (AAA→AAG)	rluE ←	23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,194,643	0	T	C	100.0%	183.0 / NA	53	K103K (AAA→AAG)	rluE	23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

GCGGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCG  >  NC_000913/1194509‑1194774                                                                                                                                       |                                                                                                                                    gcgGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTAcc                                                                                                                                >  1:149238/1‑140 (MQ=255) gcgGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTAcc                                                                                                                                <  1:622130/140‑1 (MQ=255) gcgGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTAcc                                                                                                                                >  2:815232/1‑140 (MQ=255)     gTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGc                                                                                                                            >  1:728737/1‑140 (MQ=255)     gTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGc                                                                                                                            <  1:177779/140‑1 (MQ=255)                aGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAAcg                                                                                                                 <  2:429128/140‑1 (MQ=255)                aGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAAcg                                                                                                                 <  1:815232/140‑1 (MQ=255)                  cTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAAcgcg                                                                                                               >  2:553376/1‑140 (MQ=255)                        gcCGGCGGGCAGGGTAGGGCCATCATTTAAAGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCa                                                                                                         >  1:421283/1‑140 (MQ=255)                            gcggGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTGCCCGGCGGGGTTAGACGCGCCTGCAAcgc                                                                                                     >  1:279465/1‑140 (MQ=255)                                           aagccatTTAAGGTTACGCCATTGCGCAAGGTTTCAGGGGGGTTTTGT‑TTGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGc                                                                                     <  1:62409/136‑1 (MQ=255)                                              tcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCAc                                                                                   >  2:468012/1‑140 (MQ=255)                                              tcatTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGATTAAACGGGCCGGCAACGCGCGGTTATTGGTCAGCAc                                                                                   >  1:218202/1‑140 (MQ=255)                                                      ggTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAAcc                                                                           <  2:728737/140‑1 (MQ=255)                                                      ggTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAAcc                                                                           >  2:565807/1‑140 (MQ=255)                                                       gTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAAccc                                                                          >  1:130724/1‑140 (MQ=255)                                                          aCGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCTCCGTTAGTGGTCAACACCAGCAACCCTTc                                                                       >  1:373006/1‑140 (MQ=255)                                                           cGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCTGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCg                                                                      >  2:641638/1‑140 (MQ=255)                                                           cGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCg                                                                      >  2:48517/1‑140 (MQ=255)                                                               aTTGCGCAAGGCTTCCAGTGCGCCTCGTGTGGGAATATCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTAt                                                                  <  1:231428/140‑1 (MQ=255)                                                                 tGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATcg                                                                >  2:10560/1‑140 (MQ=255)                                                                 agacagaGGCTTCAAGTTCGTCTTGTGTGGGAATATCTTACACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATcg                                                                <  2:253328/134‑1 (MQ=255)                                                                        ggCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCaa                                                         >  2:830285/1‑140 (MQ=255)                                                                         gCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAg                                                        <  1:641638/140‑1 (MQ=255)                                                                         gCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAg                                                        <  1:48517/140‑1 (MQ=255)                                                                             cAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTCCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGa                                                    >  2:510712/1‑140 (MQ=255)                                                                              aaGTGCGTCTTGTGTGGGAATGCCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAc                                                   <  1:675784/140‑1 (MQ=255)                                                                              aaGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAc                                                   <  1:491223/140‑1 (MQ=255)                                                                               aGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAcc                                                  <  1:565807/140‑1 (MQ=255)                                                                                  gCGTCTTGTGTGGGAATCCCTTCCACCTGCACATACTAGCTTTTTCCGTTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgc                                               <  2:218202/140‑1 (MQ=255)                                                                                  gCGTCTTGTGTGGGAATACCTTGCACCTGCAAATAATAGGTTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgc                                               <  2:279465/140‑1 (MQ=255)                                                                                  gCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAAACCTACGGTATCGCAGTCAAGGCgctgtct                                               >  1:253328/1‑134 (MQ=255)                                                                                   cGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctg                                                <  1:724501/138‑1 (MQ=255)                                                                                   cGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctg                                                >  2:724501/1‑138 (MQ=255)                                                                                     tCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgct                                              <  2:178133/138‑1 (MQ=255)                                                                                     tCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctgct                                              >  1:178133/1‑138 (MQ=255)                                                                                      cTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCa                                           >  2:183182/1‑140 (MQ=255)                                                                                         gtgtgGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATaa                                        <  2:421283/140‑1 (MQ=255)                                                                                              ggAATTCCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctg                                                <  1:30934/127‑1 (MQ=255)                                                                                              ggAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACctg                                                >  2:30934/1‑127 (MQ=255)                                                                                              ggAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAcc                                   <  2:149238/140‑1 (MQ=255)                                                                                              ggAATACCTTCCACCAGCACATCATCGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACAcc                                   <  1:383685/140‑1 (MQ=255)                                                                                                      ttCCACCTGCACATAATAGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCg                           >  1:827702/1‑140 (MQ=255)                                                                                                                  ataataGATTTTTCCGAGGCGCTTACCCGGCAGCGGTAACCGCGCCTGAAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCtt               <  2:373006/140‑1 (MQ=255)                                                                                                                   taataGATTTTTCCGGTGCACTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCttt              >  2:542817/1‑140 (MQ=255)                                                                                                                      taGATTTTTCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACATGCTGCATAAACACCCAGAACCGGGATGAATTCTTTTaa           >  2:350081/1‑140 (MQ=255)                                                                                                                             ttCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATGAACACCCGGAATCGGGATGAAATCTTTTAATGTTTTg    >  1:313574/1‑140 (MQ=255)                                                                                                                              tCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGGGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGc   >  2:473670/1‑140 (MQ=255)                                                                                                                              tCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGc   >  2:512398/1‑140 (MQ=255)                                                                                                                              tCCGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGAACTGCTGCATAAATACCCTGAACCGGGATGAATTCTTTTAATGTTTTGc   >  2:359634/1‑140 (MQ=255)                                                                                                                               ccGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCg  <  1:830285/140‑1 (MQ=255)                                                                                                                                cGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCg  <  1:98421/139‑1 (MQ=255)                                                                                                                                cGGTGCGCTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCg  >  2:98421/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    GCGGGTTCGTCAACCAGCTCCGCGCCGGCGGGCAGGGTAGGGCCATCATTTAAGGTTACGCCATTGCGCAAGGCTTCAAGTGCGTCTTGTGTGGGAATACCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCTGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCG  >  NC_000913/1194509‑1194774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑