| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 163,992 | G→A | A630T (GCG→ACG) | hrpB → | putative ATP‑dependent RNA helicase HrpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 163,992 | 0 | G | A | 92.3% | 68.1 / ‑2.5 | 26 | A630T (GCG→ACG) | hrpB | putative ATP‑dependent RNA helicase HrpB |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (13/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
CTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCA > NC_000913/163856‑164126 | cTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGgcggaaacgg < 1:427631/140‑1 (MQ=255) tGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGgcggaaacgga < 1:13162/140‑1 (MQ=255) gCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTAc < 1:5182/140‑1 (MQ=255) tCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGt > 2:467434/1‑140 (MQ=255) cAGTTGACGGTGAAATTGCAGCTGCGGGCTAAACCGTGAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGtt < 1:287030/140‑1 (MQ=255) gTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCt < 2:67431/128‑1 (MQ=255) gTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCt > 1:67431/1‑128 (MQ=255) aGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGt < 1:745565/140‑1 (MQ=255) gCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGc < 1:802213/140‑1 (MQ=255) gCCGCTGGCGAAACCGTAAGAATACGAGTTGCATCTGGCCATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGc < 2:332307/140‑1 (MQ=255) cAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCa < 1:467434/140‑1 (MQ=255) gacagAGTTGCATCATGCGATGATTCATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCgg < 1:387006/136‑1 (MQ=255) aTCAGGCGATGCTTAATGGCATACGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGATTATGACTGGCCAGCGGTTGATGATGaa < 1:402474/140‑1 (MQ=255) tCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGTATACGATTGGCCAGCGGTTGATGATGaaa > 1:362662/1‑140 (MQ=255) ggCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGtt > 1:703926/1‑140 (MQ=255) gCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGTAAAGTGGTAGCCAGCATATGACTGGACAGCGGTTGATGAGGaactgtc > 2:387006/1‑135 (MQ=255) gCTTAATGGCATCCGCGATAAATGTCTAAGCGTGCTCTACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTgg < 1:254519/140‑1 (MQ=255) tCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGaa > 2:716967/1‑140 (MQ=255) tCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGaa > 1:567290/1‑140 (MQ=255) gTTTAAGCGTGCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAATTTTATTGGCAGCGCTGGAAACGTGGctgctg > 2:357600/1‑140 (MQ=255) gCTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGa > 1:775577/1‑140 (MQ=255) cTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGAc > 1:777632/1‑140 (MQ=255) cTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGAc > 1:609933/1‑140 (MQ=255) cTCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTAGTGGCAGCGCTGTAAACGTGGCGGCTGCCACATATGAc > 1:303863/1‑140 (MQ=255) tCAACTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACt < 1:187469/140‑1 (MQ=255) cTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCg > 2:12038/1‑140 (MQ=255) cTGGACGGCGGAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCg < 2:501716/140‑1 (MQ=255) cggcggAAACGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACAt > 2:103440/1‑140 (MQ=255) cggaaacggaaCAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCa > 1:54365/1‑140 (MQ=255) cggaaacggaaCAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCa > 1:444270/1‑140 (MQ=255) | CTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCA > NC_000913/163856‑164126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |