Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,266,530	T→C	V146A (GTC→GCC)	prmC →	protein‑(glutamine‑N(5)) methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,266,530	0	T	C	100.0%	129.5 / NA	38	V146A (GTC→GCC)	prmC	protein‑(glutamine‑N(5)) methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/24);  total (14/24)

CAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAG  >  NC_000913/1266394‑1266663                                                                                                                                         |                                                                                                                                      cAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGcctc                                                                                                                                    >  2:483291/1‑140 (MQ=255)  aGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTcc                                                                                                                                   <  1:638610/140‑1 (MQ=255)   ggCACTGGCGCGGTTTCCTGAACAATCTTTCCGTATTATCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTccc                                                                                                                                  <  1:298923/140‑1 (MQ=255)   ggCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTccc                                                                                                                                  <  1:119316/140‑1 (MQ=255)      aCTGGCGCGGTTGCCCGAACAACCTTGCCTTATTCTCTATCTCGGGACGGGTACCGGGTCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTgg                                                                                                                               <  1:239551/140‑1 (MQ=255)        tGGCGCGGTTGCCTGAAAAACCCTGCCGTATCCTCTATCACGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGcg                                                                                                                             <  2:255237/140‑2 (MQ=255)         ggCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACGGCGAAATTATCGCTGTAGATCGTATGCCTGCAGCTGCCTCCCTGGcac                                                                                                                            >  2:386106/1‑140 (MQ=255)           cgcgGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACaa                                                                                                                          >  1:726609/1‑140 (MQ=255)               gTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTa                                                                                                                      <  1:33400/140‑1 (MQ=255)                    cTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGcc                                                                                                                 <  2:519463/140‑1 (MQ=255)                            ccTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGcaca                                                                                                                           <  2:815009/122‑1 (MQ=255)                            ccTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGcaca                                                                                                                           >  1:815009/1‑122 (MQ=255)                                 ccGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGa                                                                                                    >  1:590016/1‑140 (MQ=255)                                 ccGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCAGCCTCCCGGGCTCAGCGTAATCCCCAGCATCTGGCGa                                                                                                    >  1:225503/1‑140 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaa                                                                                               <  1:12019/140‑1 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCa                                                                                                 >  2:440277/1‑138 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCa                                                                                                 >  2:125267/1‑138 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCa                                                                                                 <  1:125267/138‑1 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCa                                                                                                 <  1:440277/138‑1 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACAGGGGCGATTGCGTTTGAGCTGGCTATCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaa                                                                                               <  2:247581/140‑1 (MQ=255)                                         tcGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAat                                                                                            >  1:539925/1‑140 (MQ=255)                                           gATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAatat                                                                                          <  1:483291/140‑1 (MQ=255)                                           gATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAatat                                                                                          <  2:590016/140‑1 (MQ=255)                                              ctcGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGAACAGCGTAATGCCCAGCATCTAGCAATCAAAAATATCca                                                                                       >  2:280501/1‑140 (MQ=255)                                                       ggTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATACCCAGCATCTGGCGATCAAAAATATCCACATTCTGCa                                                                              >  2:185087/1‑140 (MQ=255)                                                        gTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGGCCAGCATTTGGCGATCAAAAAGATCCACATTCTGCaa                                                                             >  1:337641/1‑140 (MQ=255)                                                                                  gCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCgg                                                   <  2:194351/140‑1 (MQ=255)                                                                                     agcgagcgCCCGGACTGTGAAATTATCGCTGAAGATCGTCTGCCTGATCCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGgca                                                <  2:225503/140‑1 (MQ=255)                                                                                                ggACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCGGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCga                                     <  2:136690/140‑1 (MQ=255)                                                                                                              tCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAAt                       <  1:691741/140‑1 (MQ=255)                                                                                                              tCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAAt                       <  1:15812/140‑1 (MQ=255)                                                                                                                 cTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                    <  1:185087/140‑1 (MQ=255)                                                                                                                 cTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                    <  2:593887/140‑1 (MQ=255)                                                                                                                   gTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccgcc                  <  1:536313/140‑1 (MQ=255)                                                                                                                   gTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccgcc                  >  2:824686/1‑140 (MQ=255)                                                                                                                      gATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGta               <  2:726609/140‑1 (MQ=255)                                                                                                                              gCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACg       >  2:588211/1‑140 (MQ=255)                                                                                                                                  gATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCa   <  2:599646/140‑1 (MQ=255)                                                                                                                                   aTGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAg  >  1:168050/1‑140 (MQ=255)                                                                                                                                   aTGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAg  >  2:688085/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAG  >  NC_000913/1266394‑1266663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑