| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,276,107 | A→G | R504R (CGT→CGC) | narX ← | sensory histidine kinase NarX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,276,107 | 0 | A | G | 97.5% | 135.2 / NA | 40 | R504R (CGT→CGC) | narX | sensory histidine kinase NarX |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/16); minor base T (0/1); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATC > NC_000913/1275975‑1276240 | gctgcGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTGCACTCGCTCGCGAATGATTGAGGGCTTTACGTAATGCCTCGCGGGCaa > 2:245733/1‑140 (MQ=255) tgcGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCCCTCGCTTGCGAATGTTCGAGGGCGTGACTTAATGCCTCGAGGGCAAtt > 2:367988/1‑140 (MQ=255) ggCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGcaaca > 2:783129/1‑140 (MQ=255) ggCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTCGCGAATGTTTGATGACGTTACTTAATGCCTAGCGGGCAATTTGcaaca > 2:367316/1‑140 (MQ=255) cgccgcAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGa < 2:629028/140‑1 (MQ=255) cAGCCGTTATCCTGGACGGTCATTTCGACCTGATTATAGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGa < 1:367988/140‑1 (MQ=255) ggACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCaggcga > 2:628738/1‑140 (MQ=255) aCGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCaggcgagg > 2:670183/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAATAAGCGGATTGCCTGATGCGAGGGCACCAGGCGAggcggc > 2:299193/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAATAAGCGGAATGCCTGATACGAAGGCACCAGGAGAggcggc > 2:382289/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAggcggc > 1:145120/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAggcggc > 2:668073/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAggcggc > 2:547103/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAAAAAGGGGATTGCCTGATACGAAAGCACCAGGCGAggcggc > 2:290728/1‑140 (MQ=255) tCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAAAAAGAGCATTGCCTGATCCGAATGCACCAGGCGAggcggc > 2:367849/1‑140 (MQ=255) tttGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAAtt < 2:120682/140‑1 (MQ=255) ccTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATaa < 2:41916/140‑1 (MQ=255) tGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCgg < 1:644004/140‑1 (MQ=255) gcgcCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCggg < 1:664345/140‑1 (MQ=255) cgcCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAAt > 2:129262/1‑125 (MQ=255) cgcCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAAt < 1:129262/125‑1 (MQ=255) caccacGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAAtt < 2:545253/140‑1 (MQ=255) caccacGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAAtt < 1:628738/140‑1 (MQ=255) cacGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTgg < 2:818112/140‑1 (MQ=255) cacGACTTCACTCGCGTGCGAACGTTTGAGGGCGTTAATTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTgg < 1:367849/140‑1 (MQ=255) tCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTa < 1:476193/140‑1 (MQ=255) cACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATGATCCAGCTTCAACGTGAAGCCAAATTTGGCGCTGTAc > 1:274761/1‑140 (MQ=255) cACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTAc > 2:812976/1‑140 (MQ=255) cACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTAc > 1:75135/1‑140 (MQ=255) ttGCGAATGTTTGAGGGCGTTACTTGATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGc > 2:558405/1‑140 (MQ=255) ttGAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTc > 1:480409/1‑140 (MQ=255) tGAGGGCGTTACTTAGTGCCTCTCGAGCAATTTGCAAGAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTcc < 1:290728/140‑1 (MQ=255) gAGGGCGTTACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCa < 2:480409/140‑1 (MQ=255) ttACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCgg > 1:161978/1‑140 (MQ=255) ttACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCgg > 2:532039/1‑140 (MQ=255) ttACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGATGTACTCTTCGCAACTCGCCTCCAGCGCCgg > 2:275850/1‑140 (MQ=255) tACTTAATGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGa < 1:670183/140‑1 (MQ=255) cTTAATGCCTCGCGGGCAATTTCCAACAAGTAGATCGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACg < 2:351071/140‑1 (MQ=255) tGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATc > 2:689675/1‑140 (MQ=255) tGCCTCGCGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATc > 1:732090/1‑140 (MQ=255) tGCCTCGCGGGCAATGTGCAACTAGCGGACTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATc < 1:259781/140‑1 (MQ=255) | GCTGCGGATGGCATTTTCAGGCACGCCGCAGCCGTTATCCTGGACGGTCAGTTTGACCTGATTATCGTTTTGCGCCACCGTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATC > NC_000913/1275975‑1276240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |