Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,154	A→G	V323A (GTG→GCG)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,154	0	A	G	100.0%	165.0 / NA	49	V323A (GTG→GCG)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/23);  total (26/23)

TGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCACTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGC  >  NC_000913/1297022‑1297288                                                                                                                                     |                                                                                                                                       tGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGGCATTTTTTCATTTGCGAACAGTTCGCTTGCATCGACAACGGTCGCTTCAcc                                                                                                                                 >  2:245034/1‑140 (MQ=255) tGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAATTTTTCATGTGCGAACTGTTCGCTTTCATCGACAACGGTCGCTTCAcc                                                                                                                                 >  2:213773/1‑140 (MQ=255)      gATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCa                                                                                                                            >  2:691852/1‑140 (MQ=255)       aTACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAg                                                                                                                           >  2:437364/1‑140 (MQ=255)       aTACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAg                                                                                                                           <  1:633066/140‑1 (MQ=255)             cccATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCa                                                                                                                                   >  2:56601/1‑126 (MQ=255)             cccATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCa                                                                                                                                   <  1:56601/126‑1 (MQ=255)                  aGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGgtgt                                                                                                                >  1:774069/1‑140 (MQ=255)                  aGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGCTTTTCATGTGCGAACGGTTCGCTTTCATCAACAAAGGTTGCGTCACCGAACAGAATCTTGgtgt                                                                                                                >  1:369908/1‑140 (MQ=255)                     aaCCAGTTTCTCTGCCTTTTCTTCCCCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGtttt                                                                                                             <  1:394402/140‑1 (MQ=255)                       ccAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCACTTTCATCGACAGCGGCCGCTTCACGGATCCGAATCTTGGTGTTTTCt                                                                                                           >  2:223088/1‑140 (MQ=255)                        cAGTTTCTCTGCTTTTTCTACCACGTCTTCGCAATGTTTGGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTg                                                                                                          <  2:321550/140‑1 (MQ=255)                              ctctGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACag                                                                                                    <  1:437364/140‑1 (MQ=255)                                ctGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCACGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACagag                                                                                                  <  2:647094/140‑1 (MQ=255)                                   cTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAg                                                                                               <  2:774069/140‑1 (MQ=255)                                        tCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGc                                                                                          <  2:729175/140‑1 (MQ=255)                                        tCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGCGTTTTCTGGTACAGAGAAGCCTGc                                                                                          >  2:133744/1‑140 (MQ=255)                                           aCCGCGTCTTCGAAATGCTTAGCGCGGTACATTACAAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAg                                                                                       <  2:369908/140‑1 (MQ=255)                                                gTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAg                                                                                  <  1:133744/140‑1 (MQ=255)                                                  cTTCGAAACCTTTAGCGCGGTAAATTGCCAGAGTCGGGGACAGTTTTTGATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCa                                                                                <  2:401209/140‑1 (MQ=255)                                                         aTCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTtat                                                                         >  1:683691/1‑140 (MQ=255)                                                                 cgcgGTACATTGCCATAGTCGGCGACAGTCTTTCATGCGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAggctggc                                                                 <  1:218396/140‑1 (MQ=255)                                                                    ggTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGa                                                              <  2:683691/140‑1 (MQ=255)                                                                    ggTACATTGCCAGAGGCGGGGACAGTTTTTCATGTGCAAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGa                                                              <  1:245034/140‑1 (MQ=255)                                                                      tACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGGAGCCTGCGAGTCCACCAATTTAATAGGCTGGCTGAcc                                                            >  1:312633/1‑140 (MQ=255)                                                                      tACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGAcc                                                            >  2:472401/1‑140 (MQ=255)                                                                      tACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGAcc                                                            >  1:812698/1‑140 (MQ=255)                                                                      tACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGAcc                                                            >  1:658334/1‑140 (MQ=255)                                                                           tGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGa                                                       >  1:646850/1‑140 (MQ=255)                                                                           tGCCAGAGTCGGGGACAGTTTTCCACGTGGGAACGGTCCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGa                                                       <  1:223088/140‑1 (MQ=255)                                                                             ccAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATa                                                     <  1:472401/140‑1 (MQ=255)                                                                                gagTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGcc                                                  >  2:147848/1‑140 (MQ=255)                                                                                gagTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCACTTCAGCAATTTAATGGGCTGGTTGACCAACGATAGcc                                                  >  1:332721/1‑140 (MQ=255)                                                                                gagTCGGGGACAGTTTTTCATGTGCGAACGGGTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGATAAGCCTGCCAGTGCAGAAATTTTATCGGCTCGCTGACCAACGATAGcc                                                  >  2:308566/1‑140 (MQ=255)                                                                                     ggggACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGtt                                             >  2:592344/1‑140 (MQ=255)                                                                                             tttttCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCAc                                     >  1:678218/1‑140 (MQ=255)                                                                                                 tCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGtt                                 >  2:434814/1‑140 (MQ=255)                                                                                                  cATGTGCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGttt                                <  1:147848/140‑1 (MQ=255)                                                                                                    tgtgCGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGttttt                              <  2:646850/140‑1 (MQ=255)                                                                                                     gtgCGAACGGTTCGCTTTCATCCACAACGGTTGCTTCACCGATCAGAAGCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTc                             <  2:312633/140‑1 (MQ=255)                                                                                                      tgCGAACGGTTCGCTCTCATCACCATCGGCCGCTTCAACGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCa                            <  1:213773/140‑1 (MQ=255)                                                                                                        cGAACGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAgg                          >  2:89766/1‑140 (MQ=255)                                                                                                           aCGGTTCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATa                       <  2:658334/140‑1 (MQ=255)                                                                                                               ttCGCTTTCATCAACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTAGCTGACCAACGAAAGCAGCGTTCAGTGCACGGTTTTTCAGGATAACAt                   >  2:234525/1‑140 (MQ=255)                                                                                                                       catcaACAACGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCGACGATAGCTGCGCTCAACGCACCGTTTTTCCGGATAACATCCTGAACa           >  2:250198/1‑140 (MQ=255)                                                                                                                            acaacGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCttt      <  2:678218/140‑1 (MQ=255)                                                                                                                              aacGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCa    <  1:579744/140‑1 (MQ=255)                                                                                                                                cGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGc  >  2:722201/1‑140 (MQ=255)                                                                                                                                cGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGc  >  2:635946/1‑140 (MQ=255)                                                                                                                                cGGTCGCTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGc  >  2:96067/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCGGGGACAGTTTTTCATGTGCGAACGGTTCGCTTTCATCAACAACGGTCACTTCACCGATCAGAATCTTGGTGTTTTCTGGTACAGAGAAGCCTGCCAGTTCAGCAATTTTATAGGCTGGCTGACCAACGATAGCCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGC  >  NC_000913/1297022‑1297288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑