Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,697	G→A	A267T (GCA→ACA)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,697	0	G	A	100.0%	199.0 / NA	59	A267T (GCA→ACA)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

CGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCG  >  NC_000913/1303567‑1303833                                                                                                                                   |                                                                                                                                         cGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATGTGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAAcc                                                                                                                                 <  1:346974/140‑1 (MQ=255) cGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCAGGGATTGGACAGCTATTCGTTAATGGCACATTGAAcc                                                                                                                                 >  1:255559/1‑140 (MQ=255)    aTTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTg                                                                                                                              >  2:701212/1‑140 (MQ=255)      tAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGAc                                                                                                                            >  2:693542/1‑140 (MQ=255)              gCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAGCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCtt                                                                                                                    <  1:701212/140‑1 (MQ=255)              gCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCtt                                                                                                                    <  2:783724/140‑1 (MQ=255)               ctctGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTa                                                                                                                   >  2:10679/1‑140 (MQ=255)                tctGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAAGTGTACGTCAATGGCACATTGATCCGTGACTATTCCTTAg                                                                                                                  >  2:259063/1‑140 (MQ=255)                tctGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAg                                                                                                                  >  1:732705/1‑140 (MQ=255)                  tGTTACCCGTGCTCTCTTATATGGGCCCCACATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAgtg                                                                                                                >  1:770889/1‑140 (MQ=255)                   gTTACCCGTGCTCTCTTCTATGGGCCCCGCAGTTGTCGGCATTATTACTGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAgtgt                                                                                                               <  2:255559/140‑1 (MQ=255)                   gTTACCCGTGCTCTCTTATATGGGCCCCACATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAgtgt                                                                                                               <  2:770889/140‑1 (MQ=255)                    ttACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGtt                                                                                                              >  2:809534/1‑140 (MQ=255)                            gCTCTCTTATATGGGCCCCGCATTTATCGGCATTATTCCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                                                      <  1:393907/140‑1 (MQ=255)                            gCTCCCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                                                      <  2:492154/140‑1 (MQ=255)                              tctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGAcc                                                                                                    <  2:191469/140‑1 (MQ=255)                                  ttATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGATCCGGGACTATTCGTTAGTGTTAAGCCTGACCATcc                                                                                                >  2:279647/1‑140 (MQ=255)                                  ttATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATcc                                                                                                <  2:428535/140‑1 (MQ=255)                                  ttATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATcc                                                                                                >  2:640801/1‑140 (MQ=255)                                          gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                                                        >  2:571834/1‑140 (MQ=255)                                          gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                                                        >  1:348205/1‑140 (MQ=255)                                            cccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGc                                                                                      >  1:159337/1‑140 (MQ=255)                                              cGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACAATTTCCTAGTGTTAAGACTGATCATCCTGGTTGGTGCtt                                                                                    >  2:367353/1‑140 (MQ=255)                                                 aTTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTaa                                                                                 <  2:732705/140‑1 (MQ=255)                                                      tCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAtt                                                                            <  2:348205/140‑1 (MQ=255)                                                      tCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAtt                                                                            <  2:159337/140‑1 (MQ=255)                                                          cATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGt                                                                        >  1:40848/1‑140 (MQ=255)                                                               ttaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAAt                                                                   <  1:640801/140‑1 (MQ=255)                                                               ttaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAAt                                                                   >  1:185732/1‑140 (MQ=255)                                                                    ggTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTGAAGCCTGACCATCCTGGTTGGTGCTATAACAATTTTGTTTAATGCCAt                                                              >  2:354630/1‑140 (MQ=255)                                                                    ggTTCTATGGTTATCGAAACCAGTTATGGGTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCAt                                                              <  1:325824/140‑1 (MQ=255)                                                                    ggTTCTATGGTTATCCAAACCATTTGTGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCAt                                                              <  1:276413/140‑1 (MQ=255)                                                                       tCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGt                                                           <  1:670509/140‑1 (MQ=255)                                                                        cTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATCGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTc                                                          <  1:367353/140‑1 (MQ=255)                                                                         tATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCg                                                         >  2:198197/1‑140 (MQ=255)                                                                          aTGGTTATCGAAACCCTTTATGCTTCGCCCGGGATTGCACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                                                        <  1:209127/140‑1 (MQ=255)                                                                          aTGGTTATCGAAACCATTTATGTTTAGCCTGGGATTGTACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                                                        <  1:279647/140‑1 (MQ=255)                                                                          aTGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                                                        >  1:176260/1‑140 (MQ=255)                                                                          aTGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGa                                                        <  1:693542/140‑1 (MQ=255)                                                                             gTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGAtgt                                                     <  2:654735/140‑1 (MQ=255)                                                                                aTCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCt                                                  >  2:719515/1‑140 (MQ=255)                                                                                   gAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCtata                                               <  1:809534/140‑1 (MQ=255)                                                                                      aCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGATGGTGCTTTAACCATTTTGTTTAATCCCATGCTCGATGTGCTATATGc                                            >  2:348148/1‑140 (MQ=255)                                                                                            tATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTAt                                      <  2:40848/140‑1 (MQ=255)                                                                                                  ttGCCGGGGATTGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTCATGCCATAGTCGATGTGCTATGTGCGGTTATCGAccc                                >  1:237283/1‑140 (MQ=255)                                                                                                            ttGGACAACTGTTCGTTTATGGTACATTGAACCATAACTGTTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGt                      <  2:400256/140‑1 (MQ=255)                                                                                                            ttGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGt                      <  1:611641/140‑1 (MQ=255)                                                                                                             tGGACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGtt                     <  2:560590/140‑1 (MQ=255)                                                                                                               gACAACTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTAc                   <  1:719515/140‑1 (MQ=255)                                                                                                                    cTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATa              >  2:821031/1‑140 (MQ=255)                                                                                                                    cTGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGATATATGCGGTTATCGACCCGAAAATCCGTTACTGATa              >  1:285198/1‑140 (MQ=255)                                                                                                                     tGTTCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATAc             >  1:151092/1‑140 (MQ=255)                                                                                                                       ttCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTg           >  2:34656/1‑140 (MQ=255)                                                                                                                       ttCGTTAATGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGGTATCGACCCGAAAATCCGTTACTGATACTg           >  1:62987/1‑140 (MQ=255)                                                                                                                              aTGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTcg    <  2:151092/140‑1 (MQ=255)                                                                                                                              aTGGCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTcg    >  1:581919/1‑140 (MQ=255)                                                                                                                                ggCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTcgcg  <  2:176260/140‑1 (MQ=255)                                                                                                                                ggCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTcgcg  <  1:198197/140‑1 (MQ=255)                                                                                                                                ggCACATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTcgcg  <  1:10679/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCG  >  NC_000913/1303567‑1303833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑