Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,304,226	T→C	G131G (GGT→GGC)	oppC →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,304,226	0	T	C	100.0%	163.0 / NA	46	G131G (GGT→GGC)	oppC	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/29);  total (17/29)

TGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑TAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGG  >  NC_000913/1304091‑1304361                                                                                                                                         |                                                                                                                                        tGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAg                                                                                                                                     <  1:506711/140‑1 (MQ=255)  gACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGt                                                                                                                                    <  2:94792/140‑1 (MQ=255)   aCTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACT‑GTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTgg                                                                                                                                  >  1:681294/1‑140 (MQ=255)     tCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGa                                                                                                                                 <  1:481537/140‑1 (MQ=255)      cATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACT‑GTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGAtt                                                                                                                               <  2:681294/140‑1 (MQ=255)       aTCCGGTCGCGACCTGCTTGTGCGCGTTGCGTTTGGCGGACGTATCTCTCTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGAtt                                                                                                                               <  2:316125/140‑1 (MQ=255)       aTCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCCG‑CAAAGTGGAtt                                                                                                                               <  1:793346/140‑1 (MQ=255)        tCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGGATCTCACTCATGGGCGGTGTTGCTGCCGGACCGGTGGGGGTTGTCGGCGGGACACTTTCTGGTTCTCTTTCCGGTTCGTTGGGCGGCTAAAgggagtc                                                                                                                               >  2:175582/1‑134 (MQ=255)            gTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGGATCTCACTCATGGGCGGTGTTGCTGCGGCACTGGTGGCCGTGGGCGTCGGGGCACTTTTTGGTTCGCTTTCCGGGTTTCTTGGCGG‑GCAAGGGGGTTCggga                                                                                                                          >  2:520373/1‑138 (MQ=255)             tCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTaa                                                                                                                         >  1:189739/1‑140 (MQ=255)               gcgACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGt                                                                                                                           <  1:433119/136‑1 (MQ=255)               gcgACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGt                                                                                                                           >  2:433119/1‑136 (MQ=255)                 gACCTGCTTGTGCGCGTCGCGAATGGCGGGCCTATCTCACTCATGGTCCGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAatgat                                                                                                                     <  2:209182/140‑1 (MQ=255)                             cgcgTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGt                                                                                                                 >  2:521596/1‑132 (MQ=255)                             cgcgTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGt                                                                                                                 <  1:521596/132‑1 (MQ=255)                               cgTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGaaa                                                                                                       <  1:652192/140‑1 (MQ=255)                                              gcgTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTcc                                                                                        <  1:169578/140‑1 (MQ=255)                                              gcgTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTcc                                                                                        <  2:189739/140‑1 (MQ=255)                                              gcgTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTcc                                                                                        <  2:19867/140‑1 (MQ=255)                                              gcgTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTcc                                                                                        <  1:129153/140‑1 (MQ=255)                                              gcgTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTcc                                                                                        <  2:578204/140‑1 (MQ=255)                                                   gacagctcaTGGTCGGCGTTGCTGCGGCACGGGAGGCTGCGGTCGTCGTGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTc                                                                                   <  2:393733/135‑1 (MQ=255)                                                        ctcaTGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGtt                                                                              >  1:369395/1‑140 (MQ=255)                                                         tcaTGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGTAAATCCTCAACTCCTTCCCATTCATGttc                                                                             >  1:306972/1‑140 (MQ=255)                                                           aTGGTCGGTGTTGCTGCCGCACTGGTGGCTGTGGTGGTCTGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGttctt                                                                           <  2:315462/140‑1 (MQ=255)                                                              gTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGt                                                                        >  2:548575/1‑140 (MQ=255)                                                                   tgtTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAGATCCTCAGCTCCTTGCCATTCGTGTTCTTCGTctgtc                                                                   >  1:393733/1‑136 (MQ=255)                                                                   tgtTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCAttt                                                                   >  1:680991/1‑140 (MQ=255)                                                                   tgtTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCAttt                                                                   >  1:489379/1‑140 (MQ=255)                                                                        ctgcGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTCCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTg                                                              <  1:417394/140‑1 (MQ=255)                                                                         tgcGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTgg                                                             >  2:121832/1‑140 (MQ=255)                                                                          gcGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGt                                                            <  1:112778/140‑1 (MQ=255)                                                                             gCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGAc                                                         >  2:186393/1‑140 (MQ=255)                                                                                  ggtggCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCtttt                                                    <  1:121832/140‑1 (MQ=255)                                                                                  ggtggCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCtttt                                                    >  2:727238/1‑140 (MQ=255)                                                                                      gCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCgg                                                <  1:548575/140‑1 (MQ=255)                                                                                      gCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCgg                                                <  1:200287/140‑1 (MQ=255)                                                                                        tgtgGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAGATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTc                                              >  2:133785/1‑140 (MQ=255)                                                                                                     acacTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCctgct                                 <  1:520373/140‑1 (MQ=255)                                                                                                              ggTTCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGt                        >  1:115468/1‑140 (MQ=255)                                                                                                                 tCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCct                                    >  1:571122/1‑125 (MQ=255)                                                                                                                 tCGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCct                                    <  2:571122/125‑1 (MQ=255)                                                                                                                  cGCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCg                    >  2:687434/1‑140 (MQ=255)                                                                                                                   gCTTTCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGa                   >  2:60472/1‑140 (MQ=255)                                                                                                                       tCCGGTTATCTGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAGATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAgg               <  1:133785/140‑1 (MQ=255)                                                                                                                                 tGGGCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGATGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTcc     <  2:369395/140‑1 (MQ=255)                                                                                                                                    gCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg  <  1:686321/140‑1 (MQ=255)                                                                                                                                    gCGG‑CAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg  <  2:115468/140‑1 (MQ=255)                                                                                                                                    gCGG‑CAAAGTGGATTCGATAATGATGCGTCTTCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCctgg  <  2:306972/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGCGGGCGTATCTCACTCATGGTCGGTGTTGCTGCGGCACTGGTGGCTGTGGTCGTCGGGACACTTTATGGTTCGCTTTCCGGTTATCTGGGCGG‑TAAAGTGGATTCGGTAATGATGCGTCTGCTGGAAATCCTCAACTCCTTCCCATTCATGTTCTTCGTCATTTTGCTGGTGACCTTTTTCGGTCAAAACATCCTGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGG  >  NC_000913/1304091‑1304361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑