BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,769	(T)_9→8	coding (116/144 nt)	ytiD →	protein YtiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,761	0	T	.	98.4%	504.9 / 2.2	123	coding (108/144 nt)	ytiD	protein YtiD
Reads supporting (aligned to +/- strand): ref base T (2/0); new base . (59/62); total (61/62)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

Only 100 of 130 total aligned reads displayed.

ATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATG  >  NC_000913/4556625‑4556895

attTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGtttt                                                                                                                                     >  2:1483058/1‑140 (MQ=255)

 tttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttt                                                                                                                                    >  2:225226/1‑140 (MQ=255)

  ttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGtttttt                                                                                                                                   >  2:269410/1‑140 (MQ=255)

  ttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACCTAACTATCCGGAATATTCAGAATATACTAGCGGtttttt                                                                                                                                   >  2:533211/1‑140 (MQ=255)

   tACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttttt                                                                                                                                  >  1:1289267/1‑140 (MQ=255)

           aaTTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTGGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTTGGATTATGCAGAATATACTAGCGG‑TTTTTTTTGAGTTtg                                                                                                                         >  2:626406/1‑140 (MQ=255)

           aaTTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTtg                                                                                                                         >  2:1455554/1‑140 (MQ=255)

             ttGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTtgat                                                                                                                       >  2:543485/1‑140 (MQ=255)

                tttCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTgatgat                                                                                                                    >  1:1204427/1‑140 (MQ=255)

                    aaaTAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGAttttt                                                                                                                >  1:1485492/1‑140 (MQ=255)

                      ataataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTAt                                                                                                              >  1:1100082/1‑140 (MQ=255)

                          taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGGATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCaaa                                                                                                          >  2:386828/1‑140 (MQ=255)

                          taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATGCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCaaa                                                                                                          >  1:545040/1‑140 (MQ=255)

                          taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCaaa                                                                                                          >  2:857036/1‑140 (MQ=255)

                              tGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCTGAATATACAAGCTG‑TTGTTTTTGAGCTTGAAGATTTTTATCAAATCAt                                                                                                      >  2:493853/1‑140 (MQ=255)

                              tGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCAt                                                                                                      <  1:263733/140‑1 (MQ=255)

                               ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  1:883866/1‑140 (MQ=255)

                               ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  1:882781/1‑140 (MQ=255)

                               ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  1:218969/1‑140 (MQ=255)

                               ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  2:132917/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCa                                                                                                       >  1:1244874/1‑133 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCa                                                                                                       <  2:1244874/133‑1 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  2:1522817/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  2:170105/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  1:807830/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  2:1196644/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  1:302115/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTGTTTTTGAGTTTGATGATTTTTGTCAAATCATGACttt                                                                                                >  1:638176/1‑140 (MQ=255)

                                    aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATAGACTAGCGG‑GTTTGTTTGAGTTTGATGATTTGTATCAAATCATGACttt                                                                                                >  2:411382/1‑140 (MQ=255)

                                      tATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttttt                                                                                              >  2:105741/1‑140 (MQ=255)

                                         gCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTcc                                                                                           >  2:264675/1‑140 (MQ=255)

                                         gCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTcc                                                                                           >  2:134418/1‑140 (MQ=255)

                                          cGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCt                                                                                          <  2:865222/140‑1 (MQ=255)

                                            gAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGa                                                                                        <  2:1492117/140‑1 (MQ=255)

                                              aataaataATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaa                                                                                      >  1:1528389/1‑140 (MQ=255)

                                                 aaataaTTTCCCACCGGAATTAAGTAGCAGTGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGt                                                                                   <  2:1316742/140‑1 (MQ=255)

                                                 aaataaTTTCCCACCCGAATTACGTTGCAACGGTGACCAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGt                                                                                   <  1:493853/140‑1 (MQ=255)

                                                  aataatTTCCCACCGGAATTAAGTGGCAGGGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                  <  1:626406/140‑1 (MQ=255)

                                                  aataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                  <  1:264675/140‑1 (MQ=255)

                                                   ataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCa                                                                                 <  2:1370110/140‑1 (MQ=255)

                                                   ataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCa                                                                                 <  1:1429228/140‑1 (MQ=255)

                                                   ataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCa                                                                                 <  1:1522817/140‑1 (MQ=255)

                                                         tCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat                                                                           >  1:814347/1‑140 (MQ=255)

                                                           ccACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                  <  2:1084161/131‑1 (MQ=255)

                                                            cACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATAtt                                                                        >  2:1507676/1‑140 (MQ=255)

                                                                ggAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATa                                                                    <  2:1100082/140‑1 (MQ=255)

                                                                 gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGGATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAg                                                                   <  1:386828/140‑1 (MQ=255)

                                                                  aaTTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAgg                                                                  >  2:261692/1‑140 (MQ=255)

                                                                   aTTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGc                                                                 <  1:523831/140‑1 (MQ=255)

                                                                   aTTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGc                                                                 <  1:1306312/140‑1 (MQ=255)

                                                                       aGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACATTTTTCCTGAAAAGGCAGTAGATATTCATAGGCAAGt                                                             >  1:520835/1‑140 (MQ=255)

                                                                        gTAGCAGCGGTGACACGTATTTGCATCTACGTAACTAATCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:555551/140‑1 (MQ=255)

                                                                        gTAGCAGCGGTGACACGTATTTCCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:561134/140‑1 (MQ=255)

                                                                        gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:353654/140‑1 (MQ=255)

                                                                        gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:105741/140‑1 (MQ=255)

                                                                        gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:1196644/140‑1 (MQ=255)

                                                                          agcagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAg                                                          <  2:230677/140‑1 (MQ=255)

                                                                              gcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCAGGACTTTTTCCCTGAGATATCAGTAGCTATTAGTAGGCAACTAAGGttt                                                      >  2:739140/1‑140 (MQ=255)

                                                                                   gACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATAc                                                 >  1:1039241/1‑140 (MQ=255)

                                                                                      aaGTATTTTCATCTACGTAACTATTCGGAATATTCAGTATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACttt                                              <  1:533211/140‑1 (MQ=255)

                                                                                      aaGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACttt                                              >  2:985882/1‑140 (MQ=255)

                                                                                         tATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCt                                           >  1:1104515/1‑140 (MQ=255)

                                                                                         tATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCt                                           >  1:823613/1‑140 (MQ=255)

                                                                                            tttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGAc                                        <  1:1483058/140‑1 (MQ=255)

                                                                                             ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                       <  2:814347/140‑1 (MQ=255)

                                                                                             ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                       <  2:1104515/140‑1 (MQ=255)

                                                                                             ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                       <  1:1097839/140‑1 (MQ=255)

                                                                                             ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                       <  1:1455554/140‑1 (MQ=255)

                                                                                             ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                       <  1:825907/140‑1 (MQ=255)

                                                                                             ttCATCTAAGTAACTAATCGGAGTATTCAGTCTATACTACCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAATAGTTAGTAAATATTCATAGGCACGTAAGGTTTTATACTTTGCTGACa                                       <  2:752640/140‑1 (MQ=255)

                                                                                              tCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAg                                      >  2:1036599/1‑140 (MQ=255)

                                                                                                   tACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc                                 >  2:14949/1‑140 (MQ=255)

                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  1:280050/140‑1 (MQ=255)

                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  2:770486/140‑1 (MQ=255)

                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  1:1150423/140‑1 (MQ=255)

                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  2:1204427/140‑1 (MQ=255)

                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  2:1385843/140‑1 (MQ=255)

                                                                                                       tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  2:1485492/140‑1 (MQ=255)

                                                                                                          cTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTg                          >  2:970320/1‑140 (MQ=255)

                                                                                                             ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  2:222811/134‑1 (MQ=255)

                                                                                                             ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             >  1:222811/1‑134 (MQ=255)

                                                                                                             ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct                       <  1:1036599/140‑1 (MQ=255)

                                                                                                             ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct                       <  2:1289267/140‑1 (MQ=255)

                                                                                                               cGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa                     >  1:107862/1‑140 (MQ=255)

                                                                                                                 gAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                   <  2:1216538/140‑1 (MQ=255)

                                                                                                                  aaTATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGTTTTTATACTTTGCTGATAGGATTCAGGCCTGTCTCAGAc                  >  2:492886/1‑140 (MQ=255)

                                                                                                                  aaTATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGAc                  <  1:958973/140‑1 (MQ=255)

                                                                                                                  aaTATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGAc                  <  1:299069/140‑1 (MQ=255)

                                                                                                                  aaTATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGACAAGTAAGTTTTGATAATTTGCTGACAGGAGTCAGGCCTGTCTCAGAc                  >  2:435086/1‑140 (MQ=255)

                                                                                                                   atatTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACt                 <  1:881511/140‑1 (MQ=255)

                                                                                                                     atTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTg                >  1:1306865/1‑140 (MQ=255)

                                                                                                                     atTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGACGATTTTTATCAATTCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGa               <  2:704335/140‑1 (MQ=255)

                                                                                                                     atTCAGAATATACTACCGG‑TTTATTCTGATTTTGATGTTTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGa               <  1:411382/140‑1 (MQ=255)

                                                                                                                       tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa             <  2:323557/140‑1 (MQ=255)

                                                                                                                            atatACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGGTTTATACTGTGCAGACCGGATTCAGACCTGTCTCAGACTGACATGGAt        >  2:494947/1‑140 (MQ=255)

                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                   >  1:63907/1‑125 (MQ=255)

                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa    >  1:949174/1‑140 (MQ=255)

                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa    >  2:1450538/1‑140 (MQ=255)

                                                                                                                                aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa    >  2:1530238/1‑140 (MQ=255)

                                                                                                                                  tAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATg  <  2:949174/140‑1 (MQ=255)

ATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATG  >  NC_000913/4556625‑4556895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`