Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)7→6	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,190	0	G	.	98.4%	244.1 / ‑2.5	62	intergenic (‑151/+217)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base . (29/31);  minor base A (1/0);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351052‑1351319                                                                                                                                           |                                                                                                                                  tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg                                                                                                                                  >  1:1457898/1‑140 (MQ=255)  aTTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTGTAGTTGCTAGGACCGACCGGAACAGGCTGCGGCAggg                                                                                                                                 >  1:562149/1‑140 (MQ=255)  aTTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAAAGGTTATTATAATCAACCTGGCTGTGAGTAGCTCTAGTTGCGAGGGCCGACCGGAACAGGCTGCGGCAggg                                                                                                                                 >  1:526525/1‑140 (MQ=255)     gttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggggg                                                                                                                              <  2:235445/140‑1 (MQ=255)      ttgATGCTTGTTGTGCCTGAAATTCAGGCGACTCGTCGTTTTAGTAAATAGTACGAACAGATAAAAGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggggggc                                                                                                                             <  2:526525/140‑2 (MQ=255)      ttgATGCTTGTTGTGCCTGAAACTCAGGCGAATCGGCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggggggc                                                                                                                             <  2:562149/140‑2 (MQ=255)       tgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAggggggcg                                                                                                                            >  2:1346942/1‑138 (MQ=255)          tGCTTGTTGTGCCTGCAAATCAAGCTATTCGTCGTTTAAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCtt                                                                                                                        <  1:651613/140‑1 (MQ=255)           gCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGAACGGGGCGGGCTGCGGCAGGGGgcgctttt                                                                                                                        >  1:399077/1‑136 (MQ=255)           gCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGATATAGTTGGCAGGTCCGACCGGAGCTGGCTCCGGCA‑GGGGGGCGCttt                                                                                                                       >  2:494008/1‑140 (MQ=255)              tgttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTcc                                                                                                                    >  1:1353315/1‑140 (MQ=255)              tgttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAAGTCGGACGGGAGCAGGATGCGGCA‑GGGGGGCGCTTTTcc                                                                                                                    >  2:421288/1‑140 (MQ=255)               gttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATTGTTGCCAGGTCCGACCAGAGCAGGCTGCGG‑AAGGGGGGCGCTTTTccc                                                                                                                   >  2:651613/1‑140 (MQ=255)                ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTcccc                                                                                                                  >  1:228945/1‑140 (MQ=255)                ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTcccc                                                                                                                  >  2:1256079/1‑140 (MQ=255)                 tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  2:1353315/140‑1 (MQ=255)                 tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  2:1467136/140‑1 (MQ=255)                 tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  2:1019266/140‑1 (MQ=255)                 tgtgCCTGAAAATCACGCGATTCGTCGTTCTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  1:421288/140‑1 (MQ=255)                  gtgCCTGAAAATCAGGCAATTCGTCGTTTTATTAAGCAGTACGAACAGGTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTc                                                                                                                <  2:476594/140‑1 (MQ=255)                   tgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 >  2:134594/1‑138 (MQ=255)                   tgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  1:134594/138‑1 (MQ=255)                     ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCAcc                                                                                                             >  2:295208/1‑140 (MQ=255)                     ccTGAAAATCAGACGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCAcc                                                                                                             >  1:997824/1‑140 (MQ=255)                           aaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAAcc                                                                                                       <  1:178727/140‑1 (MQ=255)                             tCAGGCGATTCGTCGATTTAGTGAAAAGTTCGAACAGTTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCct                                                                                                     <  1:585714/140‑1 (MQ=255)                                             tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  2:228945/140‑1 (MQ=255)                                             tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:1346942/140‑1 (MQ=255)                                             tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     >  2:1032441/1‑140 (MQ=255)                                                  tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                <  2:997824/140‑1 (MQ=255)                                                      cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                            >  1:1278517/1‑140 (MQ=255)                                                      cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAACGt                                                                            >  2:855856/1‑140 (MQ=255)                                                         tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCAACCTAACCATCTACCAAGAGGGGCGAGGGGACCGTAtt                                                                         >  1:480520/1‑140 (MQ=255)                                                         tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCGTTTCCCCTACCCGTAAGCCTCTCCCCAGAGGGGCCAGGGGGCCGTaat                                                                         >  2:426949/1‑138 (MQ=255)                                                          aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtg                                                                        <  2:1308303/140‑1 (MQ=255)                                                              aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGGGGGGACCGTATTGTGCa                                                                    >  1:151532/1‑140 (MQ=255)                                                               cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa                                                                   >  2:1288274/1‑140 (MQ=255)                                                                aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGAGCGAGGGGACCGTATTGTGCaaa                                                                  >  2:1442848/1‑140 (MQ=255)                                                                   tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAatat                                                               >  2:966271/1‑140 (MQ=255)                                                                           tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc                                                       <  1:1256079/140‑1 (MQ=255)                                                                           tattatAATCAACCTCGCTGTGTGTTGCTCTAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc                                                       <  1:426949/140‑1 (MQ=38)                                                                                    cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa                                              >  1:290840/1‑140 (MQ=255)                                                                                     agacaggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:680172/135‑1 (MQ=255)                                                                                     aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  2:151532/140‑1 (MQ=255)                                                                                     aaCCTGGCTGTGAGTAGCTATACTTACCATGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCACTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:731427/140‑1 (MQ=38)                                                                                       ccTGGCTGTGAGTAGCTGTAGTCGCCAGGTCTGACAGGACCAGGCTGCTGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                           <  2:480520/140‑1 (MQ=37)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                           >  2:198934/1‑140 (MQ=255)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                           >  2:1440184/1‑140 (MQ=255)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGACGGGCGGGGGGACAGTAATGTGCAAAGATTGTTACCCCAGCaacaaaca                                           >  2:545877/1‑140 (MQ=38)                                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCACTTTTCCCCTCACCCTAACCCTCTCCCCAGACGGGCGAGGGGACAGAATTGTGCAAACATTGATACCCCAGCaacaaaca                                           >  2:731427/1‑140 (MQ=38)                                                                                           gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGTATTGTGCAAATATTGTTACCCCAGCAACAAAAAGGcc                                       >  2:131802/1‑139 (MQ=255)                                                                                               tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                   <  2:1457898/140‑1 (MQ=255)                                                                                               tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                   <  1:131802/140‑1 (MQ=255)                                                                                               tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGA‑GGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc                                  <  2:788715/140‑1 (MQ=255)                                                                                                gAGTAGCTATAGTTGCCAGGTCTGACCGGCGCAGGCTTCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc                                  <  1:494008/140‑1 (MQ=38)                                                                                                 aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACa                                 <  1:198934/140‑1 (MQ=255)                                                                                                 aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCCCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTcacaca                                 >  1:1315635/1‑140 (MQ=38)                                                                                                      cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGTATTGTGCAAATATTGTAACCCCAGCCACAAACAGGCTCATAcctgtct                            >  2:680172/1‑134 (MQ=38)                                                                                                       tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATGTTGATACGCCAACATCAAACAGGCTCATACAGCCCCt                           >  1:499405/1‑140 (MQ=37)                                                                                                       tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTTTTACCCCAGCAACAAACAGGCTCATACAGCCCCt                           <  2:915753/140‑1 (MQ=255)                                                                                                       tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCt                           <  1:1032441/140‑1 (MQ=255)                                                                                                       tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCt                           <  1:295208/140‑1 (MQ=255)                                                                                                              gCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCtt                    >  2:1337609/1‑140 (MQ=255)                                                                                                              gCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAAAAAAAAGGCTCATACAGCCCCTAACCCtt                    >  2:151832/1‑140 (MQ=255)                                                                                                               ccAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCttt                   >  2:95858/1‑140 (MQ=255)                                                                                                                     ccGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc             <  2:1278517/140‑1 (MQ=255)                                                                                                                       gACCGGAGCAGGCTGAGGCA‑GGCGGACGCCTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGa           <  1:545877/140‑1 (MQ=38)                                                                                                                                aGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:290840/140‑1 (MQ=255)                                                                                                                                aGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGAGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:1442848/140‑1 (MQ=255)                                                                                                                                aGGCTGCGGCA‑GGGGGGCGCTTCTCCCCTCACCCTCACCATCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGATACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:499405/140‑1 (MQ=39)                                                                                                                                           |                                                                                                                                  TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351052‑1351319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑