BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,143,095	(G)_5→6	intergenic (‑147/‑171)	udk ← / → dgcE	uridine/cytidine kinase/putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,143,090	1	.	G	97.1%	365.5 / 2.1	105	intergenic (‑142/‑176)	udk/dgcE	uridine/cytidine kinase/putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (42/59); total (43/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 129 total aligned reads displayed.

TTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑GG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCATAAACACA  >  NC_000913/2142953‑2143224

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGTAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAGTT‑CCC‑gg‑g‑g                                                                                                                                     >  2:897666/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGGGCGCTCAGAGTAAACTGTTGAGAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  1:470587/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  1:314462/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  1:1092803/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  1:1103778/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  2:764559/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  1:902092/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g                                                                                                                                     >  2:273090/1‑140 (MQ=255)

  aGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAAAAGCGTTGCGGCGAGCTAGGAGTTAAGTGTTGACAAAGAAGT‑CCC‑gg‑g‑g                                                                                                                                     >  2:749098/1‑140 (MQ=255)

   gCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g‑g                                                                                                                                   <  1:1008553/140‑1 (MQ=255)

   gCGACCTGATTAACCTGGATGTCAGCAAGCGGGCGACCAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g‑g                                                                                                                                   <  1:445969/140‑1 (MQ=255)

    cGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGTGCGCTCAGAGTTAATTGTTGAAAAAGAATT‑CCC‑gg‑g‑g‑gg                                                                                                                                  >  2:695230/1‑139 (MQ=255)

    cGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g‑gg                                                                                                                                  >  1:991946/1‑139 (MQ=255)

    cGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g‑gg                                                                                                                                  >  1:949770/1‑139 (MQ=255)

      aCCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g‑ggca                                                                                                                                >  1:984290/1‑137 (MQ=255)

      aCCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑gg‑g‑g‑ggca                                                                                                                                >  1:68295/1‑137 (MQ=255)

      aCCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCATTGCCGCGAGCTAAGAGTTAATTGTGGACAAAGAATT‑CCC‑gg‑g‑g‑ggca                                                                                                                                >  2:636748/1‑137 (MQ=255)

         tGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAAt                                                                                                                              >  2:1420729/1‑140 (MQ=255)

           aTTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTa                                                                                                                            >  2:1080167/1‑140 (MQ=255)

           aTTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTa                                                                                                                            <  1:1080167/140‑1 (MQ=255)

           aTTAACCTGGATGTTAGCAAGGCGGCGACGAGGCGACAAACGCGGCAACTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTa                                                                                                                            <  2:447454/140‑1 (MQ=255)

             tAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACg                                                                                                                          <  2:1385960/140‑1 (MQ=255)

             tAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACg                                                                                                                          <  1:890968/140‑1 (MQ=255)

             tAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACg                                                                                                                          <  1:1154781/140‑1 (MQ=255)

             tAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACg                                                                                                                          <  1:1463190/140‑1 (MQ=255)

                 cTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGa                                                                                                                      <  1:125053/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:162950/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  2:116526/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:764559/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:1487558/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  2:96693/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  2:939065/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGGGGCGACAAAACGCTAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:749098/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGCGGCGACTAACCGCCAAACGCGCCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:437067/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGCGGCGACGAATCGCGAAACGCGACAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:576411/140‑1 (MQ=255)

                  tGGATGTTAGGAAGGCGGCGACCAATCGCCAAACGCGCCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  1:408593/140‑1 (MQ=255)

                    gATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  2:129080/138‑1 (MQ=255)

                    gATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     >  1:129080/1‑138 (MQ=255)

                    gATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     >  1:324181/1‑138 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     >  1:850084/1‑137 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     >  2:381173/1‑137 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGAt                                                                                                                     <  2:850084/137‑1 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAg                                                                                                                  >  2:386232/1‑140 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAg                                                                                                                  >  1:1343900/1‑140 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTCGAt                                                                                                                     <  1:383945/137‑1 (MQ=255)

                     aTGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTAGACAAAGCATTGCCT‑GG‑G‑G‑GCTAATTACGTTGATCAg                                                                                                                  >  1:658339/1‑140 (MQ=255)

                       gTTAGGAAGGGGGCGGCGAAGCTCCTAACCCGGCAATAATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGtt                                                                                                                <  1:429809/140‑1 (MQ=255)

                       gTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGtt                                                                                                                <  1:386232/140‑1 (MQ=255)

                         tAGGAAGGGGGCGACGAAGCACCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGtttt                                                                                                              >  1:1461296/1‑140 (MQ=255)

                          aGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGATAAAAAATG‑CCCGGG‑G‑G‑GCAAAGTACGTTGATCAGTTTTa                                                                                                             >  2:408593/1‑140 (MQ=255)

                          aGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTa                                                                                                             >  2:63593/1‑140 (MQ=255)

                          aGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTAAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTa                                                                                                             >  1:367061/1‑140 (MQ=255)

                              aGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTa                                                                                                         >  1:1375539/1‑140 (MQ=255)

                                ggggCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAg                                                                                                       >  1:1495440/1‑140 (MQ=255)

                                  ggCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGt                                                                                                     >  2:1176112/1‑140 (MQ=255)

                                                 aaCGCGGCAATTATAGGGATTTCATCCGTCTCAGAACAGACGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACg                                                                                      <  2:693023/140‑1 (MQ=255)

                                                           ttATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCa                                                                            <  2:1461296/140‑1 (MQ=255)

                                                             ataGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCaaa                                                                          <  2:984290/140‑1 (MQ=255)

                                                             ataGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCaaa                                                                          <  2:1229982/140‑1 (MQ=255)

                                                                gggATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGt                                                                                  <  1:1446137/129‑1 (MQ=255)

                                                                gggATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTa                                                                       >  2:1508049/1‑140 (MQ=255)

                                                                  gATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATc                                                                     <  1:1420729/140‑1 (MQ=255)

                                                                  gATTTCATCCGTCTGATACCAGGCGAATAGCGTTGTCGCGCGCTCAGAATTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATc                                                                     <  2:658339/140‑1 (MQ=255)

                                                                   aTTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATcc                                                                    <  1:63593/140‑1 (MQ=255)

                                                                   aTTTCATCCGTCTGATACCAGTCGAATAGCGTTGCAGCGCGCTCAGAGGTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATcc                                                                    <  2:470587/140‑1 (MQ=255)

                                                                     ttCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCATTTTTATGTAAGGCAAACAATGTTAACCGCACTTGCGCAAATTATCCGc                                                                  >  2:480396/1‑140 (MQ=255)

                                                                     ttCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGc                                                                  <  2:1103778/140‑1 (MQ=255)

                                                                     ttCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGc                                                                  >  1:899199/1‑140 (MQ=255)

                                                                     ttCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGc                                                                  >  2:1362452/1‑140 (MQ=255)

                                                                      tCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGcc                                                                 <  2:314462/140‑1 (MQ=255)

                                                                        aTCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCtt                                                               <  2:949770/140‑1 (MQ=255)

                                                                            gTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACg                                                           >  2:154187/1‑140 (MQ=255)

                                                                            gTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACg                                                           <  2:245413/140‑1 (MQ=255)

                                                                              cTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTc                                                         <  1:1362452/140‑1 (MQ=255)

                                                                                gATACCAGTCGAATAGCGTTGCCGCGCGCTAAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCAc                                                       <  2:367061/140‑1 (MQ=255)

                                                                                 aTACCAGTCGAATAGGGTTGCCCCGTGCTAAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACt                                                      <  1:480396/140‑1 (MQ=255)

                                                                                  tACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACtt                                                     <  1:819579/140‑1 (MQ=255)

                                                                                           aaTAGCGTTGCCGCGCGTTCAGTGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCaa                                            <  2:708062/140‑1 (MQ=255)

                                                                                           aaTAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCaa                                            >  1:786548/1‑140 (MQ=255)

                                                                                                     ccGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCAtata                                  <  2:325680/140‑1 (MQ=255)

                                                                                                     ccGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCAtata                                  <  1:1157806/140‑1 (MQ=255)

                                                                                                          cgcTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCAtata                                  >  1:1312300/1‑135 (MQ=255)

                                                                                                          cgcTCAGAGTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCAtata                                  <  2:1312300/135‑1 (MQ=255)

                                                                                                             tCAGAGTTTATTGTTGATAAAGAATT‑CCC‑GGTG‑C‑GAAAATTACGTTGAACAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGGAATTCGCATATAAAATGTaa                          <  2:736575/140‑1 (MQ=255)

                                                                                                             tCAGAGTTAATTGTTGACAAAGCATT‑CCC‑GGCG‑G‑GCAAATTACGTTGACCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTaa                          <  2:431580/140‑1 (MQ=255)

                                                                                                                  gTTAATTGTTGACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACtt                     <  1:1176112/140‑1 (MQ=255)

                                                                                                                       ttgttgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCGAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTa                >  1:126450/1‑140 (MQ=255)

                                                                                                                       ttgttgACAAAGAATG‑CCC‑GG‑GTG‑GCAAACTACGTTGCTCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTa                <  1:721111/140‑1 (MQ=255)

                                                                                                                         gttgACAAAGAATT‑CCCTGG‑G‑G‑TCAAATTACTTTGATCACTTTTATGTCAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACt              <  2:402429/140‑1 (MQ=255)

                                                                                                                         gttgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCGAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACt              <  2:126450/140‑1 (MQ=255)

                                                                                                                           tgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTTGCAGATGAAATGTAAAACTTTTGTACTAg            >  1:439311/1‑140 (MQ=255)

                                                                                                                           tgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTa                <  2:778976/136‑1 (MQ=255)

                                                                                                                           tgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAg            >  2:1095882/1‑140 (MQ=255)

                                                                                                                           tgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAg            >  1:317783/1‑140 (MQ=255)

                                                                                                                           tgACAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAg            >  2:1118874/1‑140 (MQ=255)

                                                                                                                             aCAAAGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCa          >  1:71096/1‑140 (MQ=255)

                                                                                                                             aCAAAGAATT‑CCC‑GG‑G‑GTGCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCa          >  2:28562/1‑140 (MQ=255)

                                                                                                                                 aGAATT‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCATaaa      <  2:1311085/140‑1 (MQ=255)

                                                                                                                                     tt‑CCCGGG‑G‑G‑GCAAATTCCGTTGATCAGTTTAATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCATAAAcaca  <  2:626419/140‑1 (MQ=255)

                                                                                                                                     tt‑CCCGGG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCATAAAcaca  <  1:897666/140‑1 (MQ=255)

TTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATT‑CCC‑GG‑G‑G‑GCAAATTACGTTGATCAGTTTTATGTAAGGTAAAAAATGTTAAACGCAGTTGCGCAAATTATCCGCCTTTACGTCACTTTATGAGCAATTCGCATATAAAATGTAAAACTTTTGTACTAGCATAAACACA  >  NC_000913/2142953‑2143224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`