BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,594	(A)_6→7	coding (888/939 nt)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,588	1	.	A	96.0%	336.8 / 4.3	99	coding (882/939 nt)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base . (1/3); new base A (41/54); total (42/57)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑AA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTT  >  NC_000913/4438450‑4438713

ttttCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑a                                                                                                                                >  1:1046107/1‑140 (MQ=255)

ttttCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑a                                                                                                                                >  2:1370727/1‑140 (MQ=255)

ttttCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑a                                                                                                                                >  2:783279/1‑140 (MQ=255)

ttttCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑a                                                                                                                                >  2:172983/1‑140 (MQ=255)

   tCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑aa‑aa                                                                                                                            >  1:1109440/1‑140 (MQ=255)

   tCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑aa‑aa                                                                                                                            >  2:869229/1‑140 (MQ=255)

   tCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATAGAAATCGACCATTGAGGCG‑TT‑T‑aa‑aa                                                                                                                            >  2:1239680/1‑140 (MQ=255)

   tCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATGTGCGAGAGATAGAATTCGACCCTTGAGGCG‑TT‑T‑aa‑aa                                                                                                                            >  1:539123/1‑140 (MQ=255)

   tCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATCTGCGAGAGATAGAATTCGACCATTGAGGCG‑TT‑T‑aa‑aa                                                                                                                            >  1:458641/1‑140 (MQ=255)

      ggTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑aa‑aaaaa                                                                                                                         <  1:26355/140‑2 (MQ=255)

       gTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑aa‑aaaaag                                                                                                                        <  1:1226132/140‑3 (MQ=255)

          aaTGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGGAATATAATTCGGCCGTTGAGGCG‑TT‑T‑aa‑aaaaagagc                                                                                                                     >  1:749745/1‑135 (MQ=255)

          aaTGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑aa‑aaaaagagc                                                                                                                     >  2:826508/1‑135 (MQ=255)

            tGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCaa                                                                                                                   >  1:931598/1‑140 (MQ=255)

                ggTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAg                                                                                                               <  2:1083789/140‑1 (MQ=255)

                ggTTGCCAACAAGTCGAATTAGCCGAACAAGTGGACGCGCTAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAg                                                                                                               <  2:628332/140‑1 (MQ=255)

                 gTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGc                                                                                                              <  2:1134761/140‑1 (MQ=255)

                 gTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGc                                                                                                              >  2:897598/1‑140 (MQ=255)

                 gTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGc                                                                                                              >  1:175268/1‑140 (MQ=255)

                 gTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATAGAAAACAAACATTGAGGTGTTT‑T‑AA‑AAAA‑GAGCAACTAGc                                                                                                              >  2:713329/1‑140 (MQ=255)

                    gCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGtt                                                                                                           <  1:382015/140‑1 (MQ=255)

                    gCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATAGAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGtt                                                                                                           <  1:1239680/140‑1 (MQ=255)

                     ccAACAAGTCGAATTTGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTa                                                                                                          <  1:675967/140‑1 (MQ=255)

                     ccAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGtt                                                                                                           <  1:1436297/139‑1 (MQ=255)

                     ccAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTa                                                                                                          <  2:931598/140‑1 (MQ=255)

                      caacaaGTCGAATTAGCTGAACGAGTGGATGTGATTGTACGTGCGAATTTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAc                                                                                                         <  2:458641/140‑1 (MQ=255)

                      caacaaGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAc                                                                                                         <  1:1518613/140‑1 (MQ=255)

                      caacaaGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAc                                                                                                         <  2:1309281/140‑1 (MQ=255)

                      caacaaGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAc                                                                                                         <  2:140362/140‑1 (MQ=255)

                      caacaaGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAc                                                                                                         >  1:793796/1‑140 (MQ=255)

                      caacaaGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAc                                                                                                         <  1:1306333/140‑1 (MQ=255)

                       aacaaGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAcc                                                                                                        <  1:869229/140‑1 (MQ=255)

                       aacaaGTCGAATTAGCCAAACAAGTGGATGCTATATTAATTGCGAATCCGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GGGCAACTAGCGTTAcc                                                                                                        <  2:405083/140‑1 (MQ=255)

                               gAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAg                                                                                                >  2:1315902/1‑140 (MQ=255)

                                aaTTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGt                                                                                               >  1:794637/1‑140 (MQ=255)

                                  ttAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTc                                                                                             <  2:793796/140‑1 (MQ=255)

                                    aGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCgg                                                                                           <  2:794637/140‑1 (MQ=255)

                                    aGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCgg                                                                                           <  1:1295252/140‑1 (MQ=255)

                                    aGCCGAACAAGTGGATGCGATACTAAGTGTGAATCTGTCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCgg                                                                                           <  1:493820/140‑1 (MQ=255)

                                       cGAACAAGTGGATGCGATAGTACGTACGACTCTGGCCCCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGt                                                                                        <  1:429424/140‑1 (MQ=255)

                                          aCAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAg                                                                                     >  1:21505/1‑140 (MQ=255)

                                            aaGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGAACATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGaa                                                                                   <  1:826508/140‑1 (MQ=255)

                                                   tGCGATAGTAAGTGCGAATCTGG‑‑GCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGAc                                                                          >  2:537655/1‑140 (MQ=255)

                                                    gCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAgaga                                                                           <  1:1226099/140‑1 (MQ=255)

                                                       aTAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACtt                                                                        >  2:1270492/1‑140 (MQ=255)

                                                        tAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GGGCAACTAGGGTTACCAGTATTAGTTGGGCGTAAGAACTCAGAGACTTa                                                                       >  2:590339/1‑140 (MQ=255)

                                                        tAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAg                                                                                     >  1:1128898/1‑126 (MQ=255)

                                                        tAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAg                                                                                     <  2:1128898/126‑1 (MQ=255)

                                                        tAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTa                                                                       >  2:237498/1‑140 (MQ=255)

                                                              gTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 <  2:1411032/140‑1 (MQ=255)

                                                              gTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 <  1:846419/140‑1 (MQ=255)

                                                              gTGCGAATCTGGCCGAAGATAGCGATATTCCCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 <  1:430080/140‑1 (MQ=255)

                                                               tGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTaa                                                                      <  2:154343/134‑1 (MQ=255)

                                                               tGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTaa                                                                      >  1:154343/1‑134 (MQ=255)

                                                               tGCGAATCTGG‑‑GCAGATAGCGATATTACCAAAGAAAAAATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTa                                                              <  1:537655/140‑1 (MQ=255)

                                                                gCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTAGCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCt                                                               <  1:1347071/140‑1 (MQ=255)

                                                                gCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCt                                                               <  2:175268/140‑1 (MQ=255)

                                                                gCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCt                                                               <  1:1315902/140‑1 (MQ=255)

                                                                gCGAATCTGGCCGCAGACAGCGATATTACCCTACACAAGTTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCt                                                               <  2:749745/140‑1 (MQ=255)

                                                                 cGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 <  1:776714/137‑1 (MQ=255)

                                                                 cGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 >  2:776714/1‑137 (MQ=255)

                                                                 cGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTa                                                              >  2:952045/1‑140 (MQ=255)

                                                                  gAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCTTTACCAGTATTAGTTCTGCGTAAGAACTCAAAGACTTAATTCCCCTAt                                                             >  2:528229/1‑140 (MQ=255)

                                                                  gAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATGAGTTCGGCGTAGGAACTCAGAGACTTAATTCCCCTAt                                                             >  1:681040/1‑140 (MQ=255)

                                                                   aaTCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 >  1:963071/1‑135 (MQ=255)

                                                                   aaTCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 <  2:963071/135‑1 (MQ=255)

                                                                   aaTCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 >  2:1542326/1‑135 (MQ=255)

                                                                   aaTCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTccc                                                                 <  1:1542326/135‑1 (MQ=255)

                                                                     tCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATccc                                                          <  1:134822/140‑1 (MQ=255)

                                                                     tCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATccc                                                          <  1:1394825/140‑1 (MQ=255)

                                                                      cTGGCCGCAGATAGCGATATTACCAAAGAAAAGGTATGCGAGAAATATTAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCa                                                         <  2:675480/140‑1 (MQ=255)

                                                                       tGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCAt                                                        >  1:968090/1‑140 (MQ=255)

                                                                           cGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGAt                                                    <  2:1380647/140‑1 (MQ=255)

                                                                              aGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAAc                                                 >  1:1322681/1‑140 (MQ=255)

                                                                                    cGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAgg                                           <  1:957851/140‑1 (MQ=255)

                                                                                       tatTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGa                                                     <  2:1159607/127‑1 (MQ=255)

                                                                                       tatTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGa                                                     >  1:1159607/1‑127 (MQ=255)

                                                                                           aCCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAat                                    >  1:1356008/1‑140 (MQ=255)

                                                                                              aaagaaaagaTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAattat                                 >  1:1468984/1‑140 (MQ=255)

                                                                                              aaagaaaagaTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAattat                                 >  1:808223/1‑140 (MQ=255)

                                                                                                   aaagaTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTg                            <  2:85764/140‑1 (MQ=255)

                                                                                                     agaTTTGCGAGAAATATAAATCTGCAATTAAGGCG‑TTAA‑AA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCa                          <  1:528229/140‑1 (MQ=255)

                                                                                                     agaTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATGACGATAGGGGAAAATTATTTTTGCa                          <  1:897598/140‑1 (MQ=255)

                                                                                                     agaTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCa                          <  1:172983/140‑1 (MQ=255)

                                                                                                     agaTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCa                          <  2:1322681/140‑1 (MQ=255)

                                                                                                      gaTTTGCGAGAAATATAAATCGTCCATTGAGGCG‑TT‑T‑AATAAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAg                         <  2:597907/140‑1 (MQ=255)

                                                                                                       aTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGa                        <  2:968090/140‑1 (MQ=255)

                                                                                                       aTTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGa                        >  1:1525214/1‑140 (MQ=255)

                                                                                                            cGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTa                   >  2:1265756/1‑140 (MQ=255)

                                                                                                            cGAGAAATATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTa                   >  2:1074682/1‑140 (MQ=255)

                                                                                                            cGAGAAATATAAATCGACCATTCAGTCG‑TT‑T‑AA‑AAAATGAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTa                   <  1:713329/140‑1 (MQ=255)

                                                                                                                 aaTATAAATCGACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTa              <  2:1525214/140‑1 (MQ=255)

                                                                                                                           gACCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCattat    <  1:1074682/140‑1 (MQ=255)

                                                                                                                            aCCATTGAGGCG‑TT‑TAAA‑AAAA‑GAGGAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCattatt   >  2:1048795/1‑140 (MQ=255)

                                                                                                                             ccATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTATCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTAttt  >  1:133972/1‑140 (MQ=255)

                                                                                                                             ccATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGTGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTa              >  2:1393265/1‑128 (MQ=255)

                                                                                                                             ccATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGTGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTa              <  1:1393265/128‑1 (MQ=255)

                                                                                                                             ccATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTAttt  >  2:1445072/1‑140 (MQ=255)

                                                                                                                             ccATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTAttt  >  1:815204/1‑140 (MQ=255)

                                                                                                                             ccATTGAGGCG‑TT‑TAAA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTAttt  >  2:906987/1‑140 (MQ=255)

TTTTCTGGTTAATGCAGGTTGCCAACAAGTCGAATTAGCCGAACAAGTGGATGCGATAGTAAGTGCGAATCTGGCCGCAGATAGCGATATTACCAAAGAAAAGATTTGCGAGAAATATAAATCGACCATTGAGGCG‑TT‑T‑AA‑AAAA‑GAGCAACTAGCGTTACCAGTATTAGTTCGGCGTAAGAACTCAGAGACTTAATTCCCCTATCCCATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTT  >  NC_000913/4438450‑4438713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`