Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2071.G88.9% 160.5 / 12.9 54noncoding (80/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base G (25/23);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606068‑4606341
                                                                                                                                             |                                                                                                                                             
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑Ga                                                                                                                                                <  1:1120964/140‑1 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑Ga                                                                                                                                                <  1:230199/140‑1 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑Ga                                                                                                                                                <  1:1202787/140‑1 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑Ga                                                                                                                                                >  2:1202787/1‑140 (MQ=255)
 tAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTTATCTTACCTTTATTAATATCGACTCGTTTGGTGC‑GA‑‑g                                                                                                                                             >  1:617867/1‑140 (MQ=18)
        aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑Ga                                                                                                                                                <  2:56435/132‑1 (MQ=255)
        aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑Ga                                                                                                                                                >  1:56435/1‑132 (MQ=255)
        aaTTGGTGCGAGGGGGGCGACTTGACCCCCCGCGTTCGTTAGGACACTTACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCGCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑g‑g‑g‑g‑g‑gg‑g                                                                                                                                <  2:476462/140‑1 (MQ=21)
             gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑g‑g‑g‑g‑g‑gg‑ggac                                                                                                                             >  2:1426968/1‑135 (MQ=255)
             gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑g‑g‑g‑g‑g‑gg‑ggac                                                                                                                             <  1:1426968/138‑4 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGa                                                                                                                          <  1:953108/140‑1 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGa                                                                                                                          <  2:1173627/140‑1 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTAGATATCGACTCGTTGGGTGCGGG‑‑G‑G‑G‑G‑G‑GG‑GACTTGa                                                                                                                          >  1:476462/1‑140 (MQ=18)
                cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATACTTTTTGTGAAATCTCCTCGTTTGGTGC‑GA‑‑G‑G‑G‑G‑G‑GG‑GGCtgtctc                                                                                                                        >  2:430892/1‑135 (MQ=18)
                      gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GATTTGAACCCCCa                                                                                                                   <  2:1529692/140‑1 (MQ=255)
                        gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACg                                                                                                                 <  2:4802/140‑1 (MQ=255)
                            cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTcc                                                                                                              <  2:1418889/140‑1 (MQ=255)
                               gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTaa                                                                                                          <  1:1075212/140‑1 (MQ=255)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAg                                                                                                         <  2:1054159/140‑1 (MQ=255)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCACCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAg                                                                                                         <  2:1333297/140‑1 (MQ=255)
                                   ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAac                                                                                                      <  1:246770/140‑1 (MQ=255)
                                     ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GT‑‑G‑G‑G‑G‑G‑GGAGACGTGAACCCCCTCGTCCGTAAGAacac                                                                                                    >  1:644996/1‑140 (MQ=25)
                                      cACGTCCGTAAGGACACCAACAGCTGAAGCTTGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTGCTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                   <  2:644996/140‑1 (MQ=37)
                                          tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                               >  2:990431/1‑140 (MQ=255)
                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTa                                                                                                           <  2:1308175/126‑1 (MQ=255)
                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTa                                                                                                           >  1:1308175/1‑126 (MQ=255)
                                                    cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGCGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     >  2:269417/1‑140 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                  <  1:269417/140‑1 (MQ=255)
                                                             cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                            >  1:1342684/1‑140 (MQ=255)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                          >  1:985488/1‑140 (MQ=255)
                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑G‑G‑G‑G‑GTGG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:54445/1‑140 (MQ=255)
                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTCAACCCCCACGTCCGGAAGACCACTAACACTTGAGGCTAGCGCGTCTACCAATTcc                                                                    >  2:1522767/1‑140 (MQ=25)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGG‑‑G‑G‑G‑G‑G‑GG‑GACTTTAACCCCCAGGTCCGCAAGAACCCGAACACCTGAACCTAGGCCGTCTACTAATTCCGc                                                                  >  2:696706/1‑140 (MQ=25)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  >  2:1523457/1‑140 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:1523457/140‑1 (MQ=255)
                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              >  2:267643/1‑140 (MQ=255)
                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              >  2:1020586/1‑140 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTCCTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  2:1342684/140‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  1:990431/140‑1 (MQ=255)
                                                                                      cccgtccTTCGCAAATTCATCTTACTTTCTTTGATCGGGACTGGTTTGAGGCGGC‑‑G‑G‑G‑G‑G‑GG‑CACTTGAAGCCCCACGTCCGTAAGAGCACTCACACCTGAAGCTAGCGCGTCTACCAATTCCGCCTCCTGCGCCCAGTg                                                   <  2:985488/135‑1 (MQ=9)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAACCTAGCGCGTCTACCAATTCCGTCACCTTCGCCCAGTg                                                   >  1:619050/1‑140 (MQ=255)
                                                                                       gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:1182609/124‑1 (MQ=255)
                                                                                       gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  >  1:1182609/1‑124 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                >  2:1255215/1‑125 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                <  1:1255215/125‑1 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGAAAGAACACTAACACCTGAAGCTAACGCATCTACCAAGTCCCGCACCTTCGCACAgctcg                                                 >  2:460602/1‑136 (MQ=21)
                                                                                           ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc                                              <  1:267643/140‑1 (MQ=255)
                                                                                           ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGc                                              >  2:836214/1‑140 (MQ=255)
                                                                                             ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa                                            >  2:1310954/1‑140 (MQ=255)
                                                                                              tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  2:1186519/137‑1 (MQ=255)
                                                                                              tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               >  1:1186519/1‑137 (MQ=255)
                                                                                                      taaTCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                   >  1:908244/3‑140 (MQ=255)
                                                                                                          cTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCTGCCACCTCCGCACAGTGCGAGTAATATCAACGTGGtt                               >  1:569704/1‑140 (MQ=21)
                                                                                                             aCTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                            <  1:1020586/140‑1 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                         <  1:338741/140‑1 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                         >  2:338741/1‑140 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                         >  1:15654/1‑140 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                         >  1:371989/1‑140 (MQ=255)
                                                                                                                  ttttGATATCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAACTCCGCCACATTCACCCCGTGCGAGCAATATGAACGTGGTTTTTGGTGc                       >  2:476690/1‑140 (MQ=21)
                                                                                                                    ttGATATCGCCTCGTTTGGTGC‑TA‑‑GTG‑G‑G‑G‑GG‑GACTTTAACCCCCACGTCCGTAAGAACACTAGCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                     <  1:696706/140‑1 (MQ=21)
                                                                                                                        tatCGCCTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                 <  1:1310954/140‑1 (MQ=255)
                                                                                                                         atCGCCTCGTTTGGTGC‑GA‑‑G‑G‑GAG‑G‑GG‑GACTGGACTCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                <  2:619050/140‑1 (MQ=21)
                                                                                                                             ccgcGTTTGGTGC‑GA‑‑G‑GTG‑G‑G‑GG‑GACTTGAAGCCCTACGCCCGTAAGATCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa            <  2:617867/137‑1 (MQ=21)
                                                                                                                             ccTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa            >  2:994486/1‑140 (MQ=255)
                                                                                                                             ccTCGTTTGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCCCAGTACGACCGATATCAACGTAGTTTCAGGTGCGAAGGGGGGGa            >  2:746453/1‑140 (MQ=17)
                                                                                                                              cTCGTTTGGTGC‑GA‑‑GAG‑G‑G‑G‑GG‑TACTTGAACCCCCACCTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc           <  1:746453/140‑1 (MQ=255)
                                                                                                                                 gTTTGGTGC‑GA‑‑G‑G‑G‑GCG‑GG‑GACCTGGACCCCCACGTCAGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg        <  1:476690/140‑1 (MQ=21)
                                                                                                                                  tttGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGTGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa       >  1:124129/1‑140 (MQ=255)
                                                                                                                                    tGGTGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAc     >  1:1137343/1‑140 (MQ=255)
                                                                                                                                       tGC‑GAG‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc  <  1:994486/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑G‑G‑G‑G‑G‑GG‑GACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606068‑4606341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑