Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,661,045	A→G	T164A (ACA→GCA)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,661,045	0	A	G	100.0%	231.7 / NA	66	T164A (ACA→GCA)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/31);  total (35/31)

CGTCGGCAAGCGCGGTGAAGGTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAACAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGTTCGGTG  >  NC_000913/1660918‑1661180                                                                                                                                |                                                                                                                                        cgTCGGCAAGCGCGGTGAAGGTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATg                                                                                                                             >  2:1494370/1‑140 (MQ=255) cgTCGGCAAGCGCGGTGAAGGTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGCACAAGTCCTGTACGGAGCAGGCGTAGATg                                                                                                                             >  1:625527/1‑140 (MQ=255)        aaGCGCGGTGAAGGTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGaaa                                                                                                                      <  1:1540667/140‑1 (MQ=255)         aGCGCGGTTAAGGTAAACTTGACCGGATAAGATCGAACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAAc                                                                                                                     <  2:697861/140‑1 (MQ=255)                     gTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCa                                                                                                         >  1:810767/1‑140 (MQ=255)                      tAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCaa                                                                                                        <  1:128164/140‑1 (MQ=255)                      tAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGACGTAGATGGCGGAAACATCACCAACTCaa                                                                                                        <  2:94965/140‑1 (MQ=255)                               aCGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGTGTAGATGGCGGAAAGATCACCTACTCAAACGTCCCGt                                                                                               >  1:401636/1‑140 (MQ=255)                                          gggACGAAGCCCTGGATTCCATGAGTGATAAACTTCGGCTGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCtgatg                                                                                    <  2:511141/140‑1 (MQ=255)                                             aCGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAAc                                                                                 >  1:1328916/1‑140 (MQ=255)                                                 aGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCtt                                                                             >  1:994467/1‑140 (MQ=255)                                                 aGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCtt                                                                             >  1:1548221/1‑140 (MQ=255)                                                  gCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTg                                                                            >  2:1165213/1‑140 (MQ=255)                                                  gCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACAGCACAAACACAAACGTCGCGTAACGTCTGATGAACTCTTg                                                                            >  2:462311/1‑140 (MQ=255)                                                    ccTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCg                                                                          <  1:12300/140‑1 (MQ=255)                                                       ggATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCggtg                                                                       <  2:994467/140‑1 (MQ=255)                                                             cATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAAGGTCCCGTACCGACTAATGAACCCTTGCGGTGGTTTtc                                                                 >  1:478723/1‑140 (MQ=255)                                                             cATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTtc                                                                 >  2:1021381/1‑140 (MQ=255)                                                             cATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTtc                                                                 >  1:1285058/1‑140 (MQ=255)                                                                cAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCa                                                              <  1:273967/140‑1 (MQ=255)                                                                  gTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCa                                                              <  2:1109211/138‑1 (MQ=255)                                                                  gTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCa                                                              >  1:1109211/1‑138 (MQ=255)                                                                  gTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGt                                                            >  2:1400679/1‑140 (MQ=255)                                                                   tGATAATCTTCGGCAGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGGGCAGGCGTAGATGGCGGAAACCTCACCAACTCAAACGTCCCATACCGTCTGATGAGATCTTGAGGTGGTTTTCTCAGTc                                                           >  2:1359863/1‑140 (MQ=255)                                                                      tAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCt                                                        <  1:178242/140‑1 (MQ=255)                                                                           ttCGGCGGATCCTGAAATATTATGGCAATGACGCTCTACCTGTCCTGTCCGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGc                                                   <  2:401636/140‑1 (MQ=255)                                                                           ttCGGCGGATCCTGAAAGATTACGGTAATAAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGc                                                   <  1:462311/140‑1 (MQ=255)                                                                           ttCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCATCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGc                                                   <  1:1494370/140‑1 (MQ=255)                                                                           ttCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCGGATGAACCCTTGCGGTGGTTTTCTCAGTCGCTATGGc                                                   >  1:664286/1‑140 (MQ=255)                                                                             cggcggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGGTGAACTCATGCTGTTGTTTTCACAGTCGCTATGGCAg                                                 >  1:455628/1‑140 (MQ=255)                                                                             cggcggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAg                                                 >  1:234368/1‑140 (MQ=255)                                                                             cggcggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAg                                                 >  2:1377491/1‑140 (MQ=255)                                                                              ggcggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGc                                                <  1:1400679/140‑1 (MQ=255)                                                                              ggcggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGc                                                >  2:183172/1‑140 (MQ=255)                                                                                cggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTa                                              <  1:1377491/140‑1 (MQ=255)                                                                                cggATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTa                                              <  2:313690/140‑1 (MQ=255)                                                                                   aTCCTGAAAGATTACGGCAATGAGGCTGTACTTGTACTGCACGGAGCACGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAg                                           <  2:423557/140‑1 (MQ=255)                                                                                   aTCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAg                                           >  1:191504/1‑140 (MQ=255)                                                                                     ccTGAAAGATTACGGCACTGAGTCTGTACATGTTCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTa                                         <  2:664286/140‑1 (MQ=255)                                                                                     ccTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTa                                         <  1:1021381/140‑1 (MQ=255)                                                                                     ccTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTa                                         <  1:183172/140‑1 (MQ=255)                                                                                        gAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCg                                      <  2:810767/140‑1 (MQ=255)                                                                                          aaGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTa                                         >  2:1311774/1‑135 (MQ=255)                                                                                          aaGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTa                                         <  1:1311774/135‑1 (MQ=255)                                                                                          aaGATTACGGCAATGAGGCTGTACATGTCCTCTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGca                                    <  2:625527/140‑1 (MQ=255)                                                                                             aTTACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGTTTTTCTCGGTCACTATGGCAGGTACAGTACCGCACAg                                 >  1:423557/1‑140 (MQ=255)                                                                                              ttACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGACACCTACCGTCTGATGAACTCTTGCGGCGGGTTTCTCAGTCGCTACGGCAGCTACAATACCGCACAGa                                >  2:322065/1‑140 (MQ=255)                                                                                               tACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGAt                               >  2:1421564/1‑140 (MQ=255)                                                                                               tACGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGAt                               >  2:152381/1‑140 (MQ=255)                                                                                                aCGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATc                              >  2:1323942/1‑140 (MQ=255)                                                                                                 cGGCAATGAGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCa                             >  1:1456053/1‑140 (MQ=255)                                                                                                         aGGCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAGCTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCa                     <  2:1525771/140‑1 (MQ=255)                                                                                                          ggCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACTGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTTGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCaa                    <  1:1359863/140‑1 (MQ=255)                                                                                                           gCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAAt                   <  2:1328916/140‑1 (MQ=255)                                                                                                           gCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAAt                   <  2:1285058/140‑1 (MQ=255)                                                                                                           gCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAAt                   <  1:1279368/140‑1 (MQ=255)                                                                                                           gCTGTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAAt                   <  2:58086/140‑1 (MQ=255)                                                                                                              gTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGAt                               >  2:894622/1‑125 (MQ=255)                                                                                                              gTACATGTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGAt                               <  1:894622/125‑1 (MQ=255)                                                                                                                  aTGTCCTGTACGGAGCATGCGTTGATGGCGGCAACTTCAGCAACTCAACCGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTtat            <  2:533137/140‑1 (MQ=255)                                                                                                                    gTCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTatat          >  1:1259033/1‑140 (MQ=255)                                                                                                                     tCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGGTACCGTACCGCACAGAGCAGTCCCGCAATGAGTTATATg         >  2:567246/1‑140 (MQ=255)                                                                                                                     tCCTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTTCAGTACCGCACAGATCAGTGCCGCAATGAGTTATATg         >  1:533137/1‑140 (MQ=255)                                                                                                                       cTGTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGtt       <  1:1214665/140‑1 (MQ=255)                                                                                                                         gTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGTTCg     >  1:1548303/1‑140 (MQ=255)                                                                                                                         gTACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGTTCg     >  1:1110468/1‑140 (MQ=255)                                                                                                                          tACGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGTTCgg    >  1:256010/1‑140 (MQ=255)                                                                                                                            cGGAGCAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGTTCGGTg  >  2:995325/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                        CGTCGGCAAGCGCGGTGAAGGTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCAGTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAACAGGCGTAGATGGCGGAAACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTACCGCACAGATCAGTGCCGCAATGAGTTATATGTTCGGTG  >  NC_000913/1660918‑1661180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑