Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,717,008	A→G	V72A (GTA→GCA)	tyrS ←	tyrosine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,717,008	0	A	G	100.0%	275.6 / NA	77	V72A (GTA→GCA)	tyrS	tyrosine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/38);  total (39/38)

ACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGC  >  NC_000913/1716875‑1717142                                                                                                                                      |                                                                                                                                       acaGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAgcg                                                                                                                                  <  1:140258/140‑1 (MQ=255) acaGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAgcg                                                                                                                                  >  2:1079687/1‑140 (MQ=255) acaGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAgcg                                                                                                                                  <  1:1242724/140‑1 (MQ=255)  caGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGACAATCAGACCCGTCGCGCCGCCTGCCAgcgc                                                                                                                                 <  1:1349526/140‑1 (MQ=255)   aGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTGGGGTCGCCAATCAGACCCGTCGCGTCGCCTGCCAGCGCa                                                                                                                                >  2:571874/1‑140 (MQ=255)   aGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCa                                                                                                                                >  2:347002/1‑140 (MQ=255)   aGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCa                                                                                                                                >  2:1251997/1‑140 (MQ=255)   aGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCa                                                                                                                                >  2:793602/1‑140 (MQ=255)    gTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCaa                                                                                                                               <  1:283413/140‑1 (MQ=255)     tCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAAc                                                                                                                              >  1:1005679/1‑140 (MQ=255)     tCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAAc                                                                                                                              >  2:1148074/1‑140 (MQ=255)      cGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAAcc                                                                                                                             <  1:1148074/140‑1 (MQ=255)      cGAAATCGAGGAACGCGGCAACCTGTTTAGGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAAcc                                                                                                                             <  1:619271/140‑1 (MQ=255)          aTCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCt                                                                                                                         >  2:1012464/1‑140 (MQ=255)               ggAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTgg                                                                                                                    >  2:785628/1‑140 (MQ=255)                gAACGGGGCAACCTGCTCACGGATTTTGTCCGCCCACTCGTGAACAGTCTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGc                                                                                                                   <  2:562887/140‑1 (MQ=255)                 aaCGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGcc                                                                                                                  <  1:793602/140‑1 (MQ=255)                    ggggCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCAGCTTGTGGCCCGc                                                                                                               <  2:184262/140‑1 (MQ=255)                     gggCAACCTGCTTACGGCTTTTCTCCACCCACTCCCGAAAAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGcc                                                                                                              <  2:396956/140‑1 (MQ=255)                        cAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTC‑‑CGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCctgctg                                                                                                         <  2:1528394/140‑1 (MQ=255)                              gCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAg                                                                                                     >  1:30305/1‑140 (MQ=255)                                  aCGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGtt                                                                                                 <  1:1012464/140‑1 (MQ=255)                                   cGGATTTTATCCACCCCATCCTGAACAGTTAGTACGGTGATCAGCTTAAGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGttt                                                                                                <  2:741613/140‑1 (MQ=255)                                    ggATTTTGTCCACCCTCTCCTGTACCGTTCCTTCGGTTTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTc                                                                                               <  1:416427/140‑1 (MQ=255)                                    ggATTTTGTCCACCCCCTCCTGCACCGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTc                                                                                               <  1:583203/140‑1 (MQ=255)                                    ggATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTc                                                                                               <  1:1517498/140‑1 (MQ=255)                                     gATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCa                                                                                              <  2:30305/140‑1 (MQ=255)                                       ttttGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAgg                                                                                            <  1:1251997/140‑1 (MQ=255)                                         ttGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCa                                                                                          >  2:1489842/1‑140 (MQ=255)                                         ttGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCa                                                                                          >  1:1325725/1‑140 (MQ=255)                                          tGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCAt                                                                                         <  2:1005679/140‑1 (MQ=255)                                                 ccacTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGGCTGATGCAAGCGTCTCAGGCATAACAATg                                                                                  >  1:711848/1‑140 (MQ=255)                                                    cTCCTGAACAGTTTCTTCGGTGCTCAGCTTACGCTCGGCCGCTTTGAATCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGaa                                                                               <  2:425557/140‑1 (MQ=255)                                                         gAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGaa                                                                               <  2:986576/135‑1 (MQ=255)                                                         gAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGaa                                                                               >  1:986576/1‑135 (MQ=255)                                                          aaCAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCGTGCTGGAAGCGATTAAGGCATAACAATGGAACAAGAt                                                                         >  1:550694/1‑140 (MQ=255)                                                              gTTTCTTCGGTGTTCAGCTTACGCTCGGCAGGTTTGAAGATCGGGTCGTCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGccc                                                                     <  2:550694/140‑1 (MQ=255)                                                               tttCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGTCCCGCCTGGTGGAAGCTTTTCAGGCATAACAATGGAACAAGATGcccc                                                                    >  2:572737/1‑140 (MQ=255)                                                               tttCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGGAGCGTTTCAGGCGTAACAATGGAACAAGATGcccc                                                                    >  2:597919/1‑140 (MQ=255)                                                               tttCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGcccc                                                                    >  1:1147123/1‑140 (MQ=255)                                                               tttCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGcccc                                                                    >  2:11049/1‑140 (MQ=255)                                                               tttCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGcccc                                                                    >  1:159024/1‑140 (MQ=255)                                                                    tcGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGTCTGCTGGACGCGCTTCCGGCATAAAGATGGAACAAGATGCCCCAAATg                                                               >  2:615950/1‑140 (MQ=255)                                                                          ttCAGCTTACGCTCGGCAGCTTGGAAGCTCGGGTCCCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCt                                                         <  2:577295/140‑1 (MQ=255)                                                                             aGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGACTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTc                                                      <  1:1079687/140‑1 (MQ=255)                                                                                  aCGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCgg                                                 >  2:154384/1‑140 (MQ=255)                                                                                  aCGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTCTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCgg                                                 >  2:513933/1‑140 (MQ=255)                                                                                      tCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGCTGCGCCAAATGCAAGCTGTCAGCGGTAgg                                             >  1:421363/1‑140 (MQ=255)                                                                                        ggCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCgg                                                 >  1:3552/1‑134 (MQ=255)                                                                                        ggCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCgg                                                 <  2:3552/134‑1 (MQ=255)                                                                                         gCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATc                                          <  1:347002/140‑1 (MQ=255)                                                                                         gCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATc                                          >  1:15912/1‑140 (MQ=255)                                                                                           aGCTTTGACGCTCGGGGGGCCAGTCAGACCCGTCGGGCCTCCTGCCAGTGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGa                                        <  2:711848/140‑1 (MQ=255)                                                                                           aGCTTTGAAGCTCGGTTCGCCAGTCTGACGCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGa                                        <  1:615950/140‑1 (MQ=255)                                                                                           aGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGa                                        <  1:11049/140‑1 (MQ=255)                                                                                           aGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGa                                        <  1:1489842/140‑1 (MQ=255)                                                                                           aGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGa                                        <  2:97799/140‑1 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAATATGCCCCAAATGCAGGCTGTCAGCGGTAGGATCGAAg                                      >  1:740273/1‑140 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTc                                                      >  1:1418275/1‑124 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTc                                                      <  2:1418275/124‑1 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAg                                      <  2:15912/140‑1 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAg                                      >  1:1463249/1‑140 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAg                                      <  2:1037690/140‑1 (MQ=255)                                                                                             cTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCCCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAg                                      >  2:1373166/1‑140 (MQ=255)                                                                                                       cGGGTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATTACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATaga                            >  2:888867/1‑140 (MQ=255)                                                                                                        gggTCGCCCATCAGACCTGTCGCGCCGCCTGACAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATagag                           <  2:740273/140‑1 (MQ=255)                                                                                                          gTCGCCAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAgcg                         >  1:1458183/1‑140 (MQ=255)                                                                                                              ccAATCAGACCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGAt                     <  1:785628/140‑1 (MQ=255)                                                                                                                      aCCCGTCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCtt             >  1:65005/1‑140 (MQ=255)                                                                                                                           tCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGcc        >  1:926103/1‑140 (MQ=255)                                                                                                                           tCGCGCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGcc        >  2:1126215/1‑140 (MQ=255)                                                                                                                            cgcgCCGCCTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGGCAGCGGTAGGATAGAAGCCGCAATAGAGCGTGATCGGGCCTTGCGCCa       >  2:419658/1‑140 (MQ=255)                                                                                                                                ccgccTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGTGGTAGGATTGAAGCCGCAATAGAGCTCGATGGTGCCTTGCGCCAGTCg   >  2:535601/1‑140 (MQ=255)                                                                                                                                ccgccTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCg   <  2:1325725/140‑1 (MQ=255)                                                                                                                                ccgccTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCg   >  1:258718/1‑140 (MQ=255)                                                                                                                                ccgccTGCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCg   >  2:1494977/1‑140 (MQ=255)                                                                                                                                 cgccTGCCAGCGCAACCCGCTTCTGGCCCGCATGCCGGAAGCGTTTCACGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGc  <  2:421363/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGC  >  NC_000913/1716875‑1717142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑