Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,718,882	T→C	T241A (ACC→GCC)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,718,882	0	T	C	100.0%	143.9 / NA	45	T241A (ACC→GCC)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/25);  total (20/25)

CCGCTCAACAAAACTTGTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCAT  >  NC_000913/1718746‑1719016                                                                                                                                         |                                                                                                                                       ccGCTCAACAAAACTTGTTCAGTAACGGTCACGGCGGGGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCt                                                                                                                                     <  1:559623/140‑1 (MQ=255)  cGCTCAACAAAACTTGTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTGCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCtt                                                                                                                                    >  1:746290/1‑140 (MQ=255)                 gTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGtt                                                                                                                       >  1:920485/1‑138 (MQ=255)                 gTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGtt                                                                                                                       <  2:920485/138‑1 (MQ=255)                 gTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCCGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAAACATACGTACAGTTTTCGCGTCGGGCGCTTTTCGGCGCAGGTTGc                                                                                                                     >  2:518346/1‑140 (MQ=255)                  ttCAGAAATGGTCACGGCGGTGCGTTCTGCCGGTGCCGCATGCACATCCCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCg                                                                                                                    <  2:396426/140‑1 (MQ=255)                       aaaTGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCATTTCGGAGCAGGTTGCGAGaaa                                                                                                               >  1:395748/1‑140 (MQ=255)                            gTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGGTCAACACCCCGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTGAAAGGATTAGCGGCCGGCGCTTTTCAGTGCCGGGTGCGAGAAATACgg                                                                                                          >  2:659860/1‑140 (MQ=255)                            gTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACgg                                                                                                          >  2:1479235/1‑140 (MQ=255)                               aCGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGGTCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCTGGGCTTTTCGGGCGAGGCCGCGAGGAATACGGGTc                                                                                                       >  2:699055/1‑140 (MQ=255)                               aCGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTc                                                                                                       <  1:322907/140‑1 (MQ=255)                                 ggcggTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGGTCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGCATTCGCGTACGGGGCTTTTCGGTGCCGGTTGCGAGAAATACGGGTCGc                                                                                                     >  2:559650/1‑140 (MQ=255)                                  gcggTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCt                                                                                                    <  1:164200/140‑1 (MQ=255)                                         gTTCTGCCAGTGTCGTCTGCACATCGCGGCGATCAACCCCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCata                                                                                             <  1:514155/140‑1 (MQ=255)                                           tCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACggg                                                                                                         >  2:366012/1‑126 (MQ=255)                                           tCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACggg                                                                                                         <  1:366012/126‑1 (MQ=255)                                             tGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGGAGGGTGCGAGAACTACGGGTCGCTGAGCATAtttt                                                                                         >  2:628754/1‑140 (MQ=255)                                               ccAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCAGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTgc                                                                                       >  1:1423914/1‑140 (MQ=255)                                                 aGTGTCGCCTGCACAGCTCGGGGATCAACCCCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTgcag                                                                                     <  1:659860/140‑1 (MQ=255)                                                  gtgtCGCCTGCACATCTGGGGGCTCAACACCTGGATAATCGCGCAAATCGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTgcagc                                                                                    <  2:395748/140‑1 (MQ=255)                                                  gtgtCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTgcagc                                                                                    <  1:1054324/140‑1 (MQ=255)                                                  gtgtCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTgcagc                                                                                    >  2:202493/1‑140 (MQ=255)                                                     tCGCCTGCACATCTCGGGGATCGACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGt                                                                                 <  1:628754/140‑1 (MQ=255)                                                       gCCTGCACATCTCGGCGATCAACACCCGGCAAATGGCTCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTgg                                                                               <  1:559650/140‑1 (MQ=255)                                                           gCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGgaga                                                                           >  2:332230/1‑140 (MQ=255)                                                           gCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGgaga                                                                           <  1:971332/140‑1 (MQ=255)                                                            cacaTCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAATTACGGGTAGCTGAGTATATTTAGCAGCAGTGGGAGaa                                                                          >  1:432672/1‑140 (MQ=255)                                                                        aTCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTAcccg                                                              >  1:122371/1‑140 (MQ=255)                                                                         tCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCGACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTAcccgc                                                             <  1:518346/140‑1 (MQ=255)                                                                         tCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTAcccgc                                                             >  2:185653/1‑140 (MQ=255)                                                                           aaCACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTAcccgccc                                                           >  1:161702/1‑140 (MQ=255)                                                                           aaCACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTAcccgccc                                                           >  1:1103212/1‑140 (MQ=255)                                                                              acCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTg                                                        <  2:161702/140‑1 (MQ=255)                                                                                ccGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGcc                                                      <  1:332230/140‑1 (MQ=255)                                                                                ccGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGcc                                                      >  2:211144/1‑140 (MQ=255)                                                                                   gAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCAc                                                   <  1:1479235/140‑1 (MQ=255)                                                                                          gcgcaaatggcgcTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCAt                                            <  1:211144/140‑1 (MQ=255)                                                                                          gcgcaaatggcgcTCCAGCCAACCATAGTTAAAGCATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCAt                                            >  2:958699/1‑140 (MQ=255)                                                                                                  ggcgcTCCAGCCAACTATAGTTGAAATATGCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCg                                    <  1:699055/140‑1 (MQ=255)                                                                                                                 cATAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGcc                     >  2:985677/1‑140 (MQ=255)                                                                                                                  aTAGTTAAAGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCt                    <  1:202493/140‑1 (MQ=255)                                                                                                                          aGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAg            >  2:1084960/1‑140 (MQ=255)                                                                                                                          aGTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAg            >  2:1081686/1‑140 (MQ=255)                                                                                                                           gTATTCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGa           <  1:1084960/140‑1 (MQ=255)                                                                                                                              ttCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATcc        >  2:1520860/1‑140 (MQ=255)                                                                                                                              ttCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCCTTATCGTAAGGTTTACCGGCCTGACGCCAGATcc        <  1:790496/140‑1 (MQ=255)                                                                                                                              ttCGCGTCCGGCGCTCTTCGGTTCACGTTCCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATcc        <  1:466538/140‑1 (MQ=255)                                                                                                                               tCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCt                    <  2:1323753/127‑1 (MQ=255)                                                                                                                               tCGCGTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCt                    >  1:1323753/1‑127 (MQ=255)                                                                                                                                cgcgTCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAg      >  1:764794/1‑140 (MQ=255)                                                                                                                                    tCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGcat  <  1:1357280/140‑1 (MQ=255)                                                                                                                                    tCCGGCGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGcat  <  1:1183659/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CCGCTCAACAAAACTTGTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCAT  >  NC_000913/1718746‑1719016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑