Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,732,427	T→C	V1114A (GTA→GCA)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,732,427	0	T	C	100.0%	170.1 / NA	48	V1114A (GTA→GCA)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/21);  total (27/21)

AATCTGCAACAAGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGC  >  NC_000913/1732291‑1732560                                                                                                                                         |                                                                                                                                      aaTCTGCAACAAGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACTTATACGCGATTGATGCTGGAACGTCAGGGCGCAtt                                                                                                                                    >  2:567909/1‑140 (MQ=255) aaTCTGCAACAAGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCAtt                                                                                                                                    >  1:132003/1‑140 (MQ=255)     tGCAACAGGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTAccc                                                                                                                                >  2:887427/1‑140 (MQ=255)      gCAACAAGTCATCTGGCTGAGCGATGAAGTATTGCCTCGACTGCGTTTACGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCg                                                                                                                               <  2:726452/140‑1 (MQ=255)      gCAACAAGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCg                                                                                                                               <  1:1211333/140‑1 (MQ=255)          caaGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCCCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCAc                                                                                                                           <  2:324909/140‑1 (MQ=255)                 tCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGt                                                                                                                    >  1:1274452/1‑140 (MQ=255)                      gTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCAGGACCGTAAGGGCGCACTACCCGCCACCGATGGTAGccc                                                                                                               >  2:518090/1‑140 (MQ=255)                       tGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCg                                                                                                              >  1:1229625/1‑140 (MQ=255)                       tGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAG‑AGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGCCGCATTACCCGCCTCCGATGGTAGCCCgg                                                                                                             >  1:548094/1‑140 (MQ=255)                         aGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATAGCTGCTGGAACGTCAGCGCGCATTAGCCGCAACCGATGGTAGCCCGgc                                                                                                            >  2:624079/1‑140 (MQ=255)                          gCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGgcg                                                                                                           <  1:91258/140‑1 (MQ=255)                           cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGgcgc                                                                                                          >  1:236301/1‑140 (MQ=255)                               gAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCtt                                                                                                      <  2:1534217/140‑1 (MQ=255)                                      ttCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCa                                                                                               <  2:236301/140‑1 (MQ=255)                                        cgtcgtCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAAc                                                                                             >  2:995194/1‑140 (MQ=255)                                        cgtcgtCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAAc                                                                                             >  2:241018/1‑140 (MQ=255)                                        cgtcgtCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAAc                                                                                             <  1:361911/140‑1 (MQ=255)                                           cgtcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATc                                                                                          >  1:1168955/1‑140 (MQ=255)                                           cgtcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATc                                                                                          >  2:1493926/1‑140 (MQ=255)                                             tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCTACCGATGGTAGGCCGGCGCTCTTTGCCTCAACATCGc                                                                                        >  1:579281/1‑140 (MQ=255)                                             tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGc                                                                                        >  1:1394287/1‑140 (MQ=255)                                             tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGc                                                                                        >  1:365320/1‑140 (MQ=255)                                              cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                       <  1:834577/140‑1 (MQ=255)                                               tGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCa                                                                                      >  2:166719/1‑140 (MQ=255)                                                         ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                            <  1:166719/140‑1 (MQ=255)                                                         ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                            <  2:337758/140‑1 (MQ=255)                                                         ctcgctGCAAGCCGCCACAGAAACGACGCGTCCCGTTGCTGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                            <  2:579281/140‑1 (MQ=255)                                                            gctGCAAGCCGCCAGAG‑AGCGACGCGTCCCGTTGCTGCCTCGACCTATTCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAggg                                                                        <  2:548094/140‑1 (MQ=255)                                                                      gccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATgg                                                               >  2:189836/1‑140 (MQ=255)                                                                       ccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGc                                                              <  1:241018/140‑1 (MQ=255)                                                                       ccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGc                                                              <  1:995194/140‑1 (MQ=255)                                                                          gagaAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTg                                                           <  2:1168955/140‑1 (MQ=255)                                                                               gCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCg                                                      >  1:1382577/1‑140 (MQ=255)                                                                                 agacagTCCCGTTGCAGGCACGACCTATGCGCGCTGGCTACTGGAACGGCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCggg                                                    <  2:687399/135‑1 (MQ=255)                                                                                  acccgTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGCGCGTAGATGGCGTGATGCgggc                                                   >  2:1226840/4‑139 (MQ=255)                                                                                  aCGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCGGACTTTTATGAGGGCGTTGATGGCGTGATGCGGGt                                                   >  2:676479/1‑140 (MQ=255)                                                                                  aCGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGt                                                   <  1:959179/140‑1 (MQ=255)                                                                                   cgcgTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTg                                                  <  2:1274452/140‑1 (MQ=255)                                                                                         ccGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGaa                                            <  2:1229625/140‑1 (MQ=255)                                                                                                 gCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGc                                    <  1:189836/140‑1 (MQ=255)                                                                                                  ccACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGTCGTAGATAGCGAGACGCGGGTGATCGAACAGCtgtct                                   >  1:687399/1‑136 (MQ=255)                                                                                                      gACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAg                               <  2:365320/140‑1 (MQ=255)                                                                                                            tGCGCGATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGtt                         >  2:1087766/1‑140 (MQ=255)                                                                                                               gcgATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTAc                      >  1:1499441/1‑140 (MQ=255)                                                                                                                cgATTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTAcc                     >  1:1226802/1‑140 (MQ=255)                                                                                                                  aTTGCTGCTGGAACGTCAGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCg                   >  1:381805/1‑140 (MQ=255)                                                                                                                     gctggtGGAACGCCGGGCCGCTTTCCCCCCCCCCGATGGTAGCCGGGCCTTTTTTGCCACAACACCCCCGGGCTTTTAGGGGGGAGTAGAGGGGGTGAGGGGGGTGATGGAACAGCTTGCGGGAGTCGGTTTACCCGCCt                <  2:1394287/140‑1 (MQ=255)                                                                                                                               cGTCAGGGCGCATTCCCCGCCCCGGATGGTAGCCCGGCGCTTTTTGCCTCAACACCGCCGGGGTTTTATGGGGGCGTAGAGGGCGTGAGGGGGGTGATCGAACAGCTTGCGGGAGTCGTTTTACCCGCCTCACTCTGGGa      <  2:381805/140‑1 (MQ=255)                                                                                                                                   aGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTCATCGAACATCTTGCCGGAGTCGGTTTACCCTCCTCACTCTGGGAAAGc  >  2:527273/1‑140 (MQ=255)                                                                                                                                   aGGGCGCATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTAATCGAACAACTTGCCGGAGTCGGTTGACCCGCCTCACTCTGGGAAAGc  >  2:493006/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AATCTGCAACAAGACATCTGGGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGC  >  NC_000913/1732291‑1732560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑