Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,852,912	T→C	G356G (GGA→GGG)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,852,912	0	T	C	98.6%	249.7 / NA	71	G356G (GGA→GGG)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (29/41);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCT  >  NC_000913/1852776‑1853047                                                                                                                                         |                                                                                                                                        tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACCTGTGTACAAAGAATATCATCACCAGCCCa                                                                                                                                      >  1:724387/1‑140 (MQ=255) tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCa                                                                                                                                      >  2:1345403/1‑140 (MQ=255) tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCa                                                                                                                                      >  1:389028/1‑140 (MQ=255) tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCa                                                                                                                                      >  2:251828/1‑140 (MQ=255) tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCa                                                                                                                                      <  1:255533/140‑1 (MQ=255) tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGAATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCa                                                                                                                                      >  1:212103/1‑140 (MQ=255)       cTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGAtt                                                                                                                                >  1:614953/1‑140 (MQ=255)        tGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTa                                                                                                                               >  2:391803/1‑140 (MQ=255)        tGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTa                                                                                                                               >  2:1354186/1‑140 (MQ=255)        tGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTa                                                                                                                               >  1:1413042/1‑140 (MQ=255)          cgacgaTCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTaaa                                                                                                                             <  2:1337390/140‑1 (MQ=255)               aTCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGc                                                                                                                        <  2:843811/140‑1 (MQ=255)               aTCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGc                                                                                                                        <  1:1245031/140‑1 (MQ=255)                 ccgtccgACGGCATTAAAGAAAGCCGAACCGCGCAGGCGTAAATGCGTGGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGccc                                                                                                                      <  2:568647/140‑1 (MQ=255)                  cgtccgACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCa                                                                                                                     <  2:83325/140‑1 (MQ=255)                    tccgACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGTCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCAct                                                                                                                   <  1:1345403/140‑1 (MQ=255)                    tccgACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCAct                                                                                                                   >  1:1002235/1‑140 (MQ=255)                    tccgACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCAct                                                                                                                   <  1:1195196/140‑1 (MQ=255)                    tccgACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCAct                                                                                                                   <  1:1161328/140‑1 (MQ=255)                      cgACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCActct                                                                                                                 <  2:1067223/140‑1 (MQ=255)                           gCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGt                                                                                                            <  2:210959/140‑1 (MQ=255)                           gCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGt                                                                                                            <  1:1262654/140‑1 (MQ=255)                                aaCGAAACCCGAACCTCGCAGGCGTAAATTCGTTGGCAAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaa                                                                                                       <  1:562740/140‑1 (MQ=255)                                aaCGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGACGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaa                                                                                                       <  2:786025/140‑1 (MQ=255)                                 aCGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaat                                                                                                      <  1:356330/140‑1 (MQ=255)                                    aaaCCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaattga                                                                                                   <  2:1372723/140‑1 (MQ=255)                                     aaCCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaattgaa                                                                                                  <  2:1002235/140‑1 (MQ=255)                                     aaCCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaattgaa                                                                                                  <  2:1373955/140‑1 (MQ=255)                                      aCCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaattgaat                                                                                                 <  1:285318/140‑1 (MQ=255)                                      aCCCGAACCGCGCAGGCCTAAATGCGTTGGCGAAACCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCtgaattgaat                                                                                                 <  2:544821/140‑1 (MQ=255)                                         cGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGa                                                                                              <  2:799955/140‑1 (MQ=255)                                           aaCCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGata                                                                                            <  1:1036642/140‑1 (MQ=255)                                                  cAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTa                                                                                     >  2:30011/1‑140 (MQ=255)                                                   aGGCGTAAATGCGTTGGTCAAACCTCCGGGAAATAGACCCCCGACGCGCAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTaa                                                                                    <  2:440429/140‑1 (MQ=255)                                                    ggCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAac                                                                                   <  2:809010/140‑1 (MQ=255)                                                      cGTAAATGCGTTGGCCATAGCTCCGGGATATGAACCGCCCACGCGAAGAAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAacac                                                                                 <  2:469145/140‑1 (MQ=255)                                                        tAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACgg                                                                               <  2:1267216/140‑1 (MQ=255)                                                           aTGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCGCTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaa                                                                            <  1:1487958/140‑1 (MQ=255)                                                               gTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataat                                                                        <  2:1267019/140‑1 (MQ=255)                                                               gTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataat                                                                        <  2:949324/140‑1 (MQ=255)                                                               gTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataat                                                                        <  1:251828/140‑1 (MQ=255)                                                                ttGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataata                                                                       >  1:975631/1‑140 (MQ=255)                                                                 tGGCCAAAGCTCCGGGATATAAACCCCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataataa                                                                      <  1:584357/140‑1 (MQ=255)                                                                     cAAAGCTCCGGGATATCAACCGCCGACGCGACGTAAACAAACATGTATTAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAAtaag                                                                  <  2:682530/140‑1 (MQ=255)                                                                      aaaGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAAtaagt                                                                 <  1:30011/140‑1 (MQ=255)                                                                          cTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAgg                                                             <  1:865600/140‑1 (MQ=255)                                                                          cTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAgg                                                             <  2:975631/140‑1 (MQ=255)                                                                             cGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAaggagg                                                          >  2:769463/1‑140 (MQ=255)                                                                                         cgccgACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAAcc                                              >  2:1280657/1‑140 (MQ=255)                                                                                           ccgACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAATACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACcga                                            >  2:1506136/1‑140 (MQ=255)                                                                                            cgACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACcgac                                           >  1:56696/1‑140 (MQ=255)                                                                                            cgACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATACAGCCTAACGCGGCAATCATAATAAGTAAGGGGGAGCCAAATAACcgac                                           >  2:611679/1‑140 (MQ=255)                                                                                                 cgAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGCAAGGTGGAGCCAAATAACAGACGAGGa                                      >  2:1218087/1‑140 (MQ=255)                                                                                                   aaGCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGaaa                                    <  1:1354186/140‑1 (MQ=255)                                                                                                     gCAAACATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAATATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAAt                                  <  2:614953/140‑1 (MQ=255)                                                                                                        aaCATACATGTATAAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGAt                               <  1:391803/140‑1 (MQ=255)                                                                                                                  tataAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAAtaattaa                     >  2:1009616/1‑140 (MQ=255)                                                                                                                  tataAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAAtaattaa                     >  1:386602/1‑140 (MQ=255)                                                                                                                   ataAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGCGCCAAATTACCGACAAGGAAATTGATCAAtaattaat                    >  1:508220/1‑140 (MQ=255)                                                                                                                    taAAAAGAAGATCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATg                   <  2:28066/140‑1 (MQ=255)                                                                                                                        aagaagATCATCTCCAGCCCATAGATTAAAATCGCCCTCTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGc               <  2:212103/140‑1 (MQ=255)                                                                                                                        aagaagATCATCACCAATCCATTGATTAAAATCCCCCACACTGTAGTCAGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGc               <  2:699276/140‑1 (MQ=255)                                                                                                                         agaagaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCa              <  2:1463914/140‑1 (MQ=255)                                                                                                                         agaagaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCa              >  1:1463914/1‑140 (MQ=255)                                                                                                                         agaagaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCa              >  2:985865/1‑140 (MQ=255)                                                                                                                            agaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATATCCGACGAGGAAAATGATCAATAATTAATGCCGCaata           >  1:676307/1‑140 (MQ=255)                                                                                                                            agaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGc                  >  2:236749/1‑133 (MQ=255)                                                                                                                            agaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGc                  <  1:236749/133‑1 (MQ=255)                                                                                                                            agaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCaata           >  1:320444/1‑140 (MQ=255)                                                                                                                             gaTCATCACCAGCCCATAGATTAAAATCGCCCACTCTATAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAAGCGACGAGCAAACTGCTCAATAGTTAATGTCGCaataa          >  1:724730/1‑140 (MQ=255)                                                                                                                                   caCCAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTc    >  1:761045/1‑140 (MQ=255)                                                                                                                                     ccAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCt  >  1:1533843/1‑140 (MQ=255)                                                                                                                                     ccAGCCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGAAAAATGAGCAATAATTAATGCTGCAATAAATATTCCt  >  1:642906/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCT  >  NC_000913/1852776‑1853047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑