Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,882,567	A→G	D219G (GAC→GGC)	dmlA →	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,882,567	0	A	G	100.0%	187.6 / NA	53	D219G (GAC→GGC)	dmlA	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/35);  total (18/35)

CCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATT  >  NC_000913/1882432‑1882700                                                                                                                                        |                                                                                                                                      ccTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAag                                                                                                                                   <  2:1080639/140‑1 (MQ=255) ccTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAag                                                                                                                                   <  1:1385201/140‑1 (MQ=255)  cTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAagc                                                                                                                                  <  2:15830/140‑1 (MQ=255)   ttCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAagca                                                                                                                                 <  2:1008500/140‑1 (MQ=255)    tCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAagcag                                                                                                                                <  2:1158687/140‑1 (MQ=255)      gAACTTGCGCAAAGCGATCCACCTAAGACACTAACTTTTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:576690/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACTCTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:583841/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:1111131/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  2:1128241/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:768418/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:1516380/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:1293912/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  1:1273181/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGTCCACATAAGACACCAACTTCTACCACTAAACCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  2:466334/140‑1 (MQ=255)      gAACTTGCGCAAAGCCGCCCACCTAAGACACCAACCTCTCCCACTAAAACGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAgcagca                                                                                                                              <  2:432070/140‑1 (MQ=255)         cTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCatat                                                                                                                           >  2:1369295/1‑140 (MQ=255)            gcgcAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGa                                                                                                                        <  1:878125/140‑1 (MQ=255)                  aGCCGTCCACGTAAGTCACTAAATTATGCTACTAAATGGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATtct                                                                                                                  <  1:658079/140‑1 (MQ=255)                  aGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATtct                                                                                                                  >  1:799528/1‑140 (MQ=255)                      gTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTgc                                                                                                              <  1:1361373/140‑1 (MQ=255)                              aaGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGttt                                                                                                      >  1:1394468/1‑140 (MQ=255)                                    cTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGAt                                                                                                <  2:1381050/140‑1 (MQ=255)                                            tGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCgg                                                                                        >  2:872545/1‑140 (MQ=255)                                            tGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCgg                                                                                        >  1:105206/1‑140 (MQ=255)                                             gCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGa                                                                                       <  1:832729/140‑1 (MQ=255)                                              ccACTAAATCGAACGGTTTAGCGATCAGCATGCGGGACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGaa                                                                                      <  2:672991/140‑1 (MQ=255)                                                   aaaTCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGAtt                                                                                 <  2:105206/140‑1 (MQ=255)                                                   aaaTCGAACGGTTTAGCCATCAACATGCCATACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGAtt                                                                                 <  1:499265/140‑1 (MQ=255)                                                      tCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGa                                                                              <  1:1369295/140‑1 (MQ=255)                                                                      tCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTcc                                                              >  1:96709/1‑140 (MQ=255)                                                                             gCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAAtttgt                                                       <  1:343834/140‑1 (MQ=255)                                                                              tcGTACTGGGATGAGCGTGTGGCAGCAATGGCCGGCAATGACCCGGAGTTCCGCTGGGGCAAGCCGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAAtttgtt                                                      <  2:405076/139‑1 (MQ=255)                                                                              ccGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAAtttgtt                                                      <  2:96709/140‑1 (MQ=255)                                                                                  aCTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGc                                                  <  1:872545/140‑1 (MQ=255)                                                                                    tGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGCCCAATTTGTTTGGCGa                                                >  1:991174/1‑140 (MQ=255)                                                                                      ggATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAAtttgtt                                                      <  2:1115963/132‑1 (MQ=255)                                                                                      ggATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAAtttgtt                                                      >  1:1115963/1‑132 (MQ=255)                                                                                       gATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGatat                                             <  1:1111411/140‑1 (MQ=255)                                                                                           aGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCtt                                         >  2:333074/1‑140 (MQ=255)                                                                                             cGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTc                                       >  1:767299/1‑140 (MQ=255)                                                                                                gTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACCATTCGATGTGGTAGTGGCGTCCAATATGTTTGGCGATATCCTTTCCGa                                    >  2:575780/1‑140 (MQ=255)                                                                                                       cAATGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGc                             >  1:1124843/1‑140 (MQ=255)                                                                                                        aaTGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGcc                            <  2:304645/140‑1 (MQ=255)                                                                                                          tGGCCGAGAATTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCg                          >  1:1136397/1‑140 (MQ=255)                                                                                                                   aTTACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCAcc                 >  2:1525414/1‑140 (MQ=255)                                                                                                                    ttACCCGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCg                <  2:1404455/140‑1 (MQ=255)                                                                                                                     tccccGGAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCgg               <  1:1511303/138‑1 (MQ=255)                                                                                                                          ggAGATCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCa          <  1:1525414/140‑1 (MQ=255)                                                                                                                            agaTCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCGTTTCCGATCTTGACCCGGCCTGCACCGGCACCAtt        >  2:504031/1‑140 (MQ=255)                                                                                                                            agaTCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCt                      >  2:769211/1‑126 (MQ=255)                                                                                                                            agaTCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCt                      <  1:769211/126‑1 (MQ=255)                                                                                                                            agaTCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGc              <  2:963047/134‑1 (MQ=255)                                                                                                                            agaTCCGCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGc              >  1:963047/1‑134 (MQ=255)                                                                                                                                  gCTGGGGCAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCAtt  >  2:1415334/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATT  >  NC_000913/1882432‑1882700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑