Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,906,322	T→C	T247A (ACA→GCA)	rlmA ←	23S rRNA m(1)G745 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,322	0	T	C	100.0%	298.1 / NA	82	T247A (ACA→GCA)	rlmA	23S rRNA m(1)G745 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/38);  total (44/38)

TTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAGTTGTTCTG  >  NC_000913/1906190‑1906456                                                                                                                                     |                                                                                                                                       ttCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAg                                                                                                                                 <  1:1462931/140‑1 (MQ=255)              ggTGCTGATCGGCATCGGCGTCGAGCTCCCCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCg                                                                                                                    <  1:721261/140‑1 (MQ=255)                     atcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCa                                                                                                             >  2:1353047/1‑140 (MQ=255)                     atcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCa                                                                                                             >  2:1386805/1‑140 (MQ=255)                          catcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGATGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCaa                                                                                                        >  1:922123/1‑140 (MQ=255)                               gcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGc                                                                                                   >  2:1494185/1‑140 (MQ=255)                                cGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGATGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCg                                                                                                  <  2:922123/140‑1 (MQ=255)                                cGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCg                                                                                                  <  1:217819/140‑1 (MQ=255)                                  tCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTc                                                                                                <  1:808725/140‑1 (MQ=255)                                   ccAGATCCTCTGGACGCACTTCAACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCa                                                                                               <  1:690743/140‑1 (MQ=255)                                    cAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCAt                                                                                              <  2:114759/140‑1 (MQ=255)                                            ctGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGTCGTCGCACGCCAAGCAATTGGCGTCATCTGCaata                                                                                      >  2:690743/1‑140 (MQ=255)                                            ctGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaata                                                                                      >  1:1417135/1‑140 (MQ=255)                                              ggACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataat                                                                                    >  1:1392987/1‑140 (MQ=255)                                              ggACGCACTTCCACGGTTAATAAGAACGCTACCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataat                                                                                    >  2:927924/1‑140 (MQ=255)                                               gACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCACTAATg                                                                                   >  1:1214990/1‑140 (MQ=255)                                               gACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATg                                                                                   >  1:370752/1‑140 (MQ=255)                                                aCGCACTTCTACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTCCCGGCCTTCTGGCTTAGCAGGCCAGGCAGACGCCGTCATCTGCATTATTGc                                                                                  >  2:417041/1‑140 (MQ=255)                                                 cGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCg                                                                                 <  1:1154946/140‑1 (MQ=255)                                                   cACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCAGCTGCAATAATGCGAc                                                                               >  1:455861/1‑140 (MQ=255)                                                    aCTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACg                                                                              >  2:1095781/1‑140 (MQ=255)                                                              ttAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCAc                                                                    >  2:790303/1‑140 (MQ=255)                                                                     gAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAg                                                             <  2:1392987/140‑1 (MQ=255)                                                                     gAACGCTGCCAGAGGCGAATATTAACGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAg                                                             <  1:417041/140‑1 (MQ=255)                                                                        cGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACg                                                          >  2:147371/1‑140 (MQ=255)                                                                        cGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACg                                                          >  1:236927/1‑140 (MQ=255)                                                                        cGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACg                                                          >  2:1184720/1‑140 (MQ=255)                                                                          cTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCATACGGCGTCATTTTCAACAATGCGACAGCTTCATCACCGCGAAGACGCa                                                        >  2:541614/1‑140 (MQ=255)                                                                               agagGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGa                                                   <  2:921215/140‑1 (MQ=255)                                                                                    tGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAaca                                              <  1:790303/140‑1 (MQ=255)                                                                                       atatTAAAGTCCGTCTGGCAGTCTAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAaca                                              >  2:1486187/1‑137 (MQ=255)                                                                                       atatTAAAGTCCGTCTGGCAGTCTAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAaca                                              <  1:1486187/137‑1 (MQ=255)                                                                                       atatTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACaa                                           >  2:1044973/1‑140 (MQ=255)                                                                                         atTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACt                                         <  1:927924/140‑1 (MQ=255)                                                                                           tAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTcc                                       <  2:1417135/140‑1 (MQ=255)                                                                                            aaaGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCg                                      <  2:370752/140‑1 (MQ=255)                                                                                             aaGTCCGTCTGGCAGTCGAACACTTCTTTTGTTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACt                                         <  1:65598/136‑1 (MQ=255)                                                                                             aaGTCCGTCTGGCAGTCGAACACTTCTTTTGTTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACt                                         >  2:65598/1‑136 (MQ=255)                                                                                             aaGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGc                                     >  1:811536/1‑140 (MQ=255)                                                                                             aaGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGc                                     >  2:221133/1‑140 (MQ=255)                                                                                             aaGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGc                                     >  2:1001893/1‑140 (MQ=255)                                                                                             aaGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCAGc                                     >  2:1540813/1‑140 (MQ=255)                                                                                              aGTCCGTCTGGCAGTGGAACACGTCTTTTTCTGCCAGGGCTTGCCAGACTTCTGGCTTCGCACGCCAGGGAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  1:522458/140‑1 (MQ=255)                                                                                              aGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  1:1001893/140‑1 (MQ=255)                                                                                              aGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  1:1230073/140‑1 (MQ=255)                                                                                              aGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  1:1353047/140‑1 (MQ=255)                                                                                              aGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  1:1494185/140‑1 (MQ=255)                                                                                              aGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  1:221133/140‑1 (MQ=255)                                                                                                tCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCAct                                  >  1:924472/1‑140 (MQ=255)                                                                                                tCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCAct                                  >  2:1299447/1‑140 (MQ=255)                                                                                                  cGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGGAAACGGCGTCATCTGCAATAATGCGCCGGCTTCAGCACCGCGAAGACGCATCGGATGACACAACTCCGCActct                                >  2:522458/1‑140 (MQ=255)                                                                                                  cGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCActct                                >  2:1335400/1‑140 (MQ=255)                                                                                                  cGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCActct                                >  2:69248/1‑140 (MQ=255)                                                                                                  cGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCActct                                >  2:871987/1‑140 (MQ=255)                                                                                                   gTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTctg                               <  1:69248/140‑1 (MQ=255)                                                                                                    tCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACTGCGAAGACGCAACGGATAACACAACTCCGCACTctgc                              >  1:634078/1‑140 (MQ=255)                                                                                                    tCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTctgc                              >  1:168893/1‑140 (MQ=255)                                                                                                    tCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTctg                               >  1:314976/1‑139 (MQ=255)                                                                                                    tCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTctg                               <  2:314976/139‑1 (MQ=255)                                                                                                    tCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGAGCAGGCGCATCGAATAAACCAACTCCCCACTctgc                              >  2:729352/1‑140 (MQ=255)                                                                                                    tCTGCCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTctgc                              >  1:895078/1‑140 (MQ=255)                                                                                                      tGGCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTctgctg                            <  1:871987/140‑1 (MQ=255)                                                                                                       ggCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCtgt                           <  1:1386805/140‑1 (MQ=255)                                                                                                        gCAGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCCTCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCtgta                          <  2:924472/140‑1 (MQ=255)                                                                                                          aGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    >  1:1392893/1‑128 (MQ=255)                                                                                                          aGTCGAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCa                                    <  2:1392893/128‑1 (MQ=255)                                                                                                              gAACACTTCTTTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCtgtaatgtaa                    >  2:997672/1‑140 (MQ=255)                                                                                                                   cttcttTTGCTGCCAGTTCTTGTCAGACTTCGGGCTTCGAACGCCAGGAAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCt               <  2:642746/140‑1 (MQ=255)                                                                                                                   cttcttTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCt               <  1:1184720/140‑1 (MQ=255)                                                                                                                    ttcttTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCtt              >  1:167791/1‑140 (MQ=255)                                                                                                                    ttcttTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCtt              >  1:1462553/1‑140 (MQ=255)                                                                                                                     tcttTTGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAACACGCATCGGATAGCACGACTCCGCAATCTGATGTAATGTAAAACCTTc             >  2:505039/1‑140 (MQ=255)                                                                                                                     tcttTTGCTGCCAGTACTTGCCAGACTTCCGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTc             <  1:424430/140‑1 (MQ=255)                                                                                                                        tttGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAg          <  2:168893/140‑1 (MQ=255)                                                                                                                        tttGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAg          <  2:811536/140‑1 (MQ=255)                                                                                                                        tttGCTGCCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAg          <  1:1299447/140‑1 (MQ=255)                                                                                                                        tttGCTGCCAGTGCTTGCCAGACTTATGGTTTCGCACGCCAGGCAAACGGCGTCATTTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAg          <  1:541614/140‑1 (MQ=255)                                                                                                                            ctgcCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAgttgt      >  2:772149/1‑140 (MQ=255)                                                                                                                            ctgcCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAgttgt      <  1:147371/140‑1 (MQ=255)                                                                                                                              gcCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTaaaa                  >  2:1052515/1‑126 (MQ=255)                                                                                                                              gcCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTaaaa                  <  1:1052515/126‑1 (MQ=255)                                                                                                                              gcCAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAGTTGTTc    >  2:1533199/1‑140 (MQ=255)                                                                                                                                cAGTGCTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAGTTGTTCTg  >  2:290610/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCGACGGCTTCATCACCGCGAAGACGCATCGGATAACACAACTCCGCACTCTGCTGTAATGTAAAACCTTCCAGTTGTTCTG  >  NC_000913/1906190‑1906456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑