Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,921,384	G→A	A388T (GCC→ACC)	rsmF →	16S rRNA m(5)C1407 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,921,384	0	G	A	98.5%	215.2 / NA	67	A388T (GCC→ACC)	rsmF	16S rRNA m(5)C1407 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (29/35);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACC  >  NC_000913/1921253‑1921516                                                                                                                                    |                                                                                                                                     cTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACg                                                                                                                              <  2:1460590/140‑1 (MQ=255) cTACAGGTGTTGGCTGAAACTGAGATGAATACCTGCGTCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACg                                                                                                                              <  1:597407/140‑1 (MQ=255)  tACAGGTGTTGGTTTAACCTGGGATGAAATCCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCACGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGAGATTAAACTTGCCGAGACGc                                                                                                                             <  2:207581/140‑1 (MQ=255)       gtgtTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCacaac                                                                                                                        <  1:360563/140‑1 (MQ=255)        tggtGGCTTAATCTGGGATGAAAACCTTCGCCTTTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTCCCGAGACGCacaaca                                                                                                                       <  2:1358346/137‑1 (MQ=255)         gtTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCAcaacaa                                                                                                                      <  1:1363349/140‑1 (MQ=255)           tGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCGCGGTTGGCGATAAACCTTACCTAGACGCACAACAAAg                                                                                                                    >  1:685695/1‑140 (MQ=255)                tAAACTGGGATGAAATCCTGCGTCTTTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTAt                                                                                                               <  1:403129/140‑1 (MQ=255)                tAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTAt                                                                                                               <  1:206176/140‑1 (MQ=255)                 aaaCTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATc                                                                                                              >  2:1334845/1‑140 (MQ=255)                 aaaCTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATc                                                                                                              >  1:146463/1‑140 (MQ=255)                       ggATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGgc                                                                                                        >  1:1094961/1‑140 (MQ=255)                       ggATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGgc                                                                                                        >  1:157043/1‑140 (MQ=255)                        gATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGgca                                                                                                       >  2:1505614/1‑140 (MQ=255)                        gATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGgca                                                                                                       >  1:1533404/1‑140 (MQ=255)                              aaCCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGa                                                                                                 <  2:157043/140‑1 (MQ=255)                                ccTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAg                                                                                               <  1:1172880/140‑1 (MQ=255)                                   gcgcCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCgg                                                                                            >  1:239182/1‑140 (MQ=255)                                   gcgcCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCgg                                                                                            >  1:1138813/1‑140 (MQ=255)                                   gcgcCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCgg                                                                                            >  1:808555/1‑140 (MQ=255)                                      ccTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTa                                                                                         <  1:1524834/140‑1 (MQ=255)                                      ccTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTa                                                                                         <  2:91412/140‑1 (MQ=255)                                      ccTCTGGCAGCGTGACAAAGAACTGTGGTTGATCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTa                                                                                         <  2:548867/140‑1 (MQ=255)                                      ccTCTGGCAGCGTGACAAAGAACTGAGGTGGTTCCCGTTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTa                                                                                         <  1:728921/140‑1 (MQ=255)                                       ctctGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTAt                                                                                        <  1:779946/140‑1 (MQ=255)                                         ctGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTTCCGAGACGAACATCAACAGTTATCGATGGCAACATGAAGGGGTTATTg                                                                                      >  2:728921/1‑140 (MQ=255)                                           ggCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAg                                                                                               >  1:529180/1‑129 (MQ=255)                                           ggCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAg                                                                                               <  2:529180/129‑1 (MQ=255)                                                cGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAg                                                                                               <  1:1439198/124‑1 (MQ=255)                                                cGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAg                                                                                               >  2:1439198/1‑124 (MQ=255)                                                 gTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGcc                                                                              <  1:1334845/140‑1 (MQ=255)                                                 gTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGcc                                                                              <  2:1533404/140‑1 (MQ=255)                                                    aCAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCa                                                                           >  2:913010/1‑140 (MQ=255)                                                     cAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCAc                                                                          <  2:1138813/140‑1 (MQ=255)                                                     cAAAGAACCGTGGTTGTTCCCGTTGGGCATTCACGACCTGATCGGTAATGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCAc                                                                          <  2:685695/140‑1 (MQ=255)                                                         gAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGa                                                                      <  1:913010/140‑1 (MQ=255)                                                          aaCTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGat                                                                     <  2:1094961/140‑1 (MQ=255)                                                          aaCTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGat                                                                     >  2:1114159/1‑140 (MQ=255)                                                               tgGTTTTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCa                                                                           >  1:1097200/1‑129 (MQ=255)                                                               tgGTTTTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCa                                                                           <  2:1097200/129‑1 (MQ=255)                                                                  ttgttCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGaa                                                             >  2:353859/1‑140 (MQ=255)                                                                        ccGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCAtt                                                       <  1:353859/140‑1 (MQ=255)                                                                         cGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAAAGCATTc                                                      >  2:922328/1‑140 (MQ=255)                                                                            tGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCAAGAAGCGGTTATTACTCATGCCTCACCCGATAATATGAATGCATTCGAg                                                   >  2:555306/1‑140 (MQ=255)                                                                                cATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGa                                               >  2:1149744/1‑140 (MQ=255)                                                                                cATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGa                                               >  2:163494/1‑140 (MQ=255)                                                                                cATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTACTCATGCCTCACCCGACAATACGAATGCATTCGAGCTGa                                               >  2:529281/1‑140 (MQ=255)                                                                                 aTTGCAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTTCCGAGACACACAACAAAGGTTATCGCTGGCAGTATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTggc                                              <  2:783736/140‑3 (MQ=255)                                                                                     aaGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCg                                          >  1:48983/1‑140 (MQ=255)                                                                                         ccTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAgg                                      <  2:808555/140‑1 (MQ=255)                                                                                         ccTGATCGGTAAAGTCCGATTTCCTCGGTTGGGGAATAATCTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAgg                                      <  2:605300/140‑1 (MQ=255)                                                                                          cTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGa                                     >  1:322956/1‑140 (MQ=255)                                                                                           tGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGaa                                    <  2:48983/140‑1 (MQ=255)                                                                                            gATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAgg                                      >  2:1324738/1‑137 (MQ=255)                                                                                            gATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAgg                                      <  1:1324738/137‑1 (MQ=255)                                                                                            gATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAg                                   <  2:33826/140‑1 (MQ=255)                                                                                             aTCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGc                                  >  2:778730/1‑140 (MQ=255)                                                                                               cGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCgg                                <  2:146463/140‑1 (MQ=255)                                                                                               cGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCACTTGCCTAACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCgg                                <  1:1411540/140‑1 (MQ=255)                                                                                                 gTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCggag                              <  2:1051889/140‑1 (MQ=255)                                                                                                          gATTTTCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTAt                     >  2:959101/1‑140 (MQ=255)                                                                                                            ttttCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGAATTTGAGTTGACACCGCAGGAGGCGGAGGAGTGGTATcg                   >  2:612888/1‑140 (MQ=255)                                                                                                            ttttCTCGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCCTGCCTCACCCGATAGTATGAATGCATTTGAGTTGACACCGGAGGACGCGGAGGAGCGGTATcg                   >  2:573725/1‑140 (MQ=255)                                                                                                                  cGGTTGGGGATTAAACTTACCGCGACGCACAACAAAGTTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGgcg             <  1:398003/140‑1 (MQ=255)                                                                                                                  cGGTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGgcg             <  2:239182/140‑1 (MQ=255)                                                                                                                   ggTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAAAGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGgcgc            <  1:922328/140‑1 (MQ=255)                                                                                                                    gTTGGGGATTAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGcgcg           <  1:1505614/140‑1 (MQ=255)                                                                                                                             tAAACTTACCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGGACCCAAGGAGTGCTATCGGGGGCGCGCTGTTTAcc  >  2:728459/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     CTACAGGTGTTGGCTTAAACTGGGATGAAAACCTGCGCCTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACC  >  NC_000913/1921253‑1921516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑