Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,933,049	A→G	V519A (GTG→GCG)	edd ←	phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,933,049	0	A	G	100.0%	202.3 / NA	58	V519A (GTG→GCG)	edd	phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/38);  total (20/38)

GCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGAC  >  NC_000913/1932917‑1933185                                                                                                                                     |                                                                                                                                         gcCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGGGTTGCGTGGATAGGTGACGGCGCTTTAcc                                                                                                                                   >  1:477055/1‑140 (MQ=255)  ccAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTG‑CGGCGCTTTACCTg                                                                                                                                 <  2:1489318/140‑1 (MQ=255)   cAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTg                                                                                                                                 <  1:337285/140‑1 (MQ=255)   cAGTTCCGCTTCGTCAACCAGCAGCGTCATTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTg                                                                                                                                 <  1:547809/140‑1 (MQ=255)     gTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGaa                                                                                                                               <  2:65559/140‑1 (MQ=255)           cTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCg                                                                                                                         >  2:1219087/1‑140 (MQ=255)            ttCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCgg                                                                                                                        >  1:915137/1‑140 (MQ=255)             tCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGga                                                                                                                       <  2:1221589/140‑1 (MQ=255)                tCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGagag                                                                                                                    <  1:1021991/140‑1 (MQ=255)                tCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGagag                                                                                                                    >  1:86049/1‑140 (MQ=255)                     cagcagCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAgtcgtc                                                                                                               <  1:1026956/140‑1 (MQ=255)                      agcagcGTCAGTTCGCCTGTCTCTCCATTGACACGAACGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTcc                                                                                                              <  1:740860/140‑1 (MQ=255)                           cGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAgtcgt                                                                                                                <  2:896840/133‑1 (MQ=255)                           cGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAgtcgt                                                                                                                >  1:896840/1‑133 (MQ=255)                             tCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGta                                                                                                       >  1:904317/1‑140 (MQ=255)                                    gCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACg                                                                                                <  2:246518/140‑1 (MQ=255)                                       tgtctgtcCATTCACACGAATGATGCCCCCGTCGCGCTCTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACACaa                                                                                             <  1:548666/140‑1 (MQ=255)                                         tctgtcCATTCACACGAATGATGTCCTCGTCGCGCACTTTTGCCAGCAGCTCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAAtt                                                                                           >  1:994531/1‑140 (MQ=255)                                          ctgtcCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAAttt                                                                                          <  2:915137/140‑1 (MQ=255)                                                  tCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAacac                                                                                  <  2:904317/140‑1 (MQ=255)                                                  tCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAacac                                                                                  <  2:1211078/140‑1 (MQ=255)                                                  tCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCGGAAGCGCCGGAGGGTCATCCATCGGAAACTAACGCAATTTTGAAacac                                                                                  >  2:394540/1‑140 (MQ=255)                                                  tCACACGAATGATGTCCCCGTCCCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAacac                                                                                  <  1:477890/140‑1 (MQ=255)                                                  tCACACGAATGATGTCCACGTCTCGCACTTTCGCCGGCAACCCGCCATAGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAacac                                                                                  <  2:477055/140‑1 (MQ=255)                                                   cacaCGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACAcc                                                                                 >  2:769279/1‑140 (MQ=255)                                                     cacGAATGATGTCCCCGTCGCGCACTTTTGCTAGCCGCCCGCCATCGTGGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGTAATTTTGAAACACCgg                                                                               <  2:408763/140‑1 (MQ=255)                                                       cGAATGATGTCCCCGACGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             <  1:394540/140‑1 (MQ=255)                                                          atgatgTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCaa                                                                          <  2:138627/140‑1 (MQ=255)                                                            gatgTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCaata                                                                        <  1:206915/140‑1 (MQ=255)                                                             atgTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             >  1:1326242/1‑134 (MQ=255)                                                             atgTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             <  2:1488319/134‑1 (MQ=255)                                                             atgTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             <  2:1326242/134‑1 (MQ=255)                                                             atgTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             >  1:1488319/1‑134 (MQ=255)                                                               gTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAataata                                                                     <  1:148629/140‑1 (MQ=255)                                                                tCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATac                                                                    >  1:787328/1‑140 (MQ=255)                                                                   ccGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTACCTAACGCAATTATGGAACACCTGTCCAATAATACAcc                                                                 >  1:645344/1‑140 (MQ=255)                                                                    cGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCa                                                                >  2:1036704/1‑140 (MQ=255)                                                                     gTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCaa                                                               >  1:37102/1‑140 (MQ=255)                                                                      tCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             >  1:882161/1‑125 (MQ=255)                                                                      tCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTc                                                                             <  2:882161/125‑1 (MQ=255)                                                                       cgcgCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGt                                                             >  1:1123076/1‑140 (MQ=255)                                                                          gcACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCTGTAACTAAAGCAATTTAGAAACACCGGTCCAATAATACACCAAGTggc                                                          >  2:610150/1‑140 (MQ=255)                                                                          gcACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTggc                                                          <  2:787328/140‑1 (MQ=255)                                                                             cTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTggcggc                                                       <  1:987552/140‑1 (MQ=255)                                                                              ttttGCCAGCAGCTCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCa                                                      <  2:994531/140‑1 (MQ=255)                                                                              ttttGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCa                                                      <  2:42221/140‑1 (MQ=255)                                                                              ttttGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCa                                                      <  2:86049/140‑1 (MQ=255)                                                                              ttttGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCa                                                      <  1:1036704/140‑1 (MQ=255)                                                                              ttttGCCAGCAGCCCGCCATCGTAGCCTTGTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCa                                                      <  1:610150/140‑1 (MQ=255)                                                                              ttttGCCAGCAGCCCCCCATCGTAGACTTTTGGTGTTCCGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCa                                                      <  1:416673/140‑1 (MQ=255)                                                                                           ccGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTaa                                         <  2:1123076/140‑1 (MQ=255)                                                                                           ccGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTaa                                         <  1:330135/140‑1 (MQ=255)                                                                                                           cTGGTGTTACGTGGATAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTc                         >  1:272355/1‑140 (MQ=255)                                                                                                                         aTAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCtt                     <  2:1073653/130‑1 (MQ=255)                                                                                                                         aTAGCTGACGGCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCtt                     >  1:1073653/1‑130 (MQ=255)                                                                                                                            gCTGACGGCGCTTTACCAGAAGCGCCGGAGAATCGCCCATCGGTAACTCACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCtgatg        <  2:645344/140‑1 (MQ=255)                                                                                                                                  ggCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGac  <  2:59171/140‑1 (MQ=255)                                                                                                                                  ggCGCTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGac  <  1:769279/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGAC  >  NC_000913/1932917‑1933185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑