Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,019,197	T→C	V271A (GTG→GCG)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,019,197	0	T	C	100.0%	149.5 / NA	43	V271A (GTG→GCG)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/19);  total (24/19)

GCCGCACCTGTTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGTTAGGG  >  NC_000913/2019064‑2019333                                                                                                                                      |                                                                                                                                         gccgcACCTGTTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAAt                                                                                                                                    <  2:1204951/140‑1 (MQ=255)  ccgcACCTGTTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAAtc                                                                                                                                   <  1:210352/140‑1 (MQ=255)         tGTTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGTCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCa                                                                                                                            <  2:1244259/140‑1 (MQ=255)                aGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGga                                                                                                                     >  1:895411/1‑140 (MQ=255)                     aCCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGGACCCGCAGTATTCAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATa                                                                                                                >  2:551240/1‑140 (MQ=255)                                     gAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCa                                                                                                <  1:1366607/140‑1 (MQ=255)                                      aaTGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAg                                                                                               >  1:1282142/1‑140 (MQ=255)                                        tGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCCCCCTCAAAGTGAATCATAACCGGGCGCAAATCCAGAt                                                                                             >  1:481942/1‑140 (MQ=255)                                          gCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGgcg                                                                                                        >  2:19998/1‑127 (MQ=255)                                          gCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGgcg                                                                                                        <  1:19998/127‑1 (MQ=255)                                                 tCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAAGCTCCCTCACAGTCGGTGATAACCAGTCGCAATTCCAGATGGTTTCAcc                                                                                    >  2:667874/1‑140 (MQ=255)                                                   aTTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTTCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGc                                                                                  >  1:285011/1‑140 (MQ=255)                                                     tAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCAt                                                                                <  2:1282142/140‑1 (MQ=255)                                                      aaGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATc                                                                               >  2:275017/1‑140 (MQ=255)                                                           aGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCAc                                                                                     <  1:172738/129‑1 (MQ=255)                                                           aGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCAc                                                                                     >  2:172738/1‑129 (MQ=255)                                                           aGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCAt                                                                          >  2:1325009/1‑140 (MQ=255)                                                           aGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACACGCAGGATTTAGGCGAAGCGCAAATCTTCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGAATCAGCAt                                                                          >  1:1020354/1‑140 (MQ=255)                                                             cATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACAAGGCGCAGATCGAGATGGTTTGACCGCATCAGCATGt                                                                        >  1:433550/1‑140 (MQ=255)                                                                  ttCGCTGTTCACCCGTCAGGGGTAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTAcgcg                                                                   <  1:667874/140‑1 (MQ=255)                                                                  ttCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTAcgcg                                                                   <  1:781944/140‑1 (MQ=255)                                                                  ttCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTAcgcg                                                                   >  2:864872/1‑140 (MQ=255)                                                                    cGCTGTTCACCCGCCAGGGGCAGCAGAGTCCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGcgcc                                                                 <  1:551240/140‑1 (MQ=255)                                                                       tGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCgccgcc                                                              >  2:1084261/1‑140 (MQ=255)                                                                           cACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTgg                                                          >  1:326070/1‑140 (MQ=255)                                                                                  cAGGGGCAACAAAGTTCAGAGTCGCATCTACACCCGCGGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAagcagc                                                   <  1:469723/140‑1 (MQ=255)                                                                                  cAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAagcagc                                                   <  1:275017/140‑1 (MQ=255)                                                                                       gCAACAAAGTGCAGAGTTGCGTTTGCACCCGCAGGTTTTAGGTGAAGCACAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTCCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGc                                              <  2:563726/140‑1 (MQ=255)                                                                                                tGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTgc                                     >  2:964266/1‑140 (MQ=255)                                                                                                   agagTGGCGTCTGCACACGCAGGATGTAGGTGAAGCGCAAATCGACCTCAAAGTGGATGAGAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATCTACGCGCCGCCCTGGAAGCAGCGCGGCCGGTACTgcgga                                  <  2:1020354/140‑3 (MQ=255)                                                                                                     agTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTGCGCGCGGCTCTGGAAGAAGCGGTGCCGGTAATGcgcacg                                >  2:713388/1‑140 (MQ=255)                                                                                                     agTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCg                                            <  2:868589/128‑1 (MQ=255)                                                                                                     agTTGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCg                                            >  1:868589/1‑128 (MQ=255)                                                                                                        tGCGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAg                             <  1:864872/140‑1 (MQ=255)                                                                                                          cGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCt                           >  1:1221564/1‑140 (MQ=255)                                                                                                          cGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCt                           >  1:1224491/1‑140 (MQ=255)                                                                                                          cGTCTGCACCCGCAGGATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCt                           >  1:882323/1‑140 (MQ=255)                                                                                                                         gATTTAGGTGAAGCGCACATCTTCCTCAAAGCGGATGATTACCAGGCGTAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCCCGCAGCTGGCCGAAAGTGGCAt            <  2:481942/140‑1 (MQ=255)                                                                                                                         gATTTAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCAt            <  2:882323/140‑1 (MQ=255)                                                                                                                           tttAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTc          >  1:367522/1‑140 (MQ=255)                                                                                                                            ttAGGTGAAGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCGAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCa         <  2:1221564/140‑1 (MQ=255)                                                                                                                              aGGTGAAGCGCAAATCTCCCTCGAAATGGTTGATAACTAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGt       <  1:571820/140‑1 (MQ=255)                                                                                                                                   aaGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTCCTGGGCAAGCAGCTGGGCGAAGGTGGCATTCAGTTAggg  >  2:640301/1‑140 (MQ=255)                                                                                                                                   aaGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGTTAggg  >  2:798941/1‑140 (MQ=255)                                                                                                                                   aaGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGTTAggg  <  2:1447766/140‑1 (MQ=255)                                                                                                                                   aaGCGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGTTAggg  <  1:964266/140‑1 (MQ=255)                                                                                                                                   aaGCGCAAATCTCCCGCAAAGTCGACGATCACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGTTAggg  <  1:640301/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GCCGCACCTGTTTTGAGTGCACCGCTGGGTTCTCACGAATGGCAACAATCATTAAGCCAGCATATTTCGCTGTTCACCCGCCAGGGGCAACAAAGTGCAGAGTTGCGTCTGCACCCGCAGGATTTAGGTGAAGTGCAAATCTCCCTCAAAGTGGATGATAACCAGGCGCAAATCCAGATGGTTTCACCGCATCAGCATGTACGCGCCGCCCTGGAAGCAGCGCTGCCGGTACTGCGCACGCAGCTGGCCGAAAGTGGCATTCAGTTAGGG  >  NC_000913/2019064‑2019333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑