Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,231,507	T→C	H163H (CAT→CAC)	yohK →	PF04712 family membrane protein YohK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,231,507	0	T	C	100.0%	132.5 / NA	39	H163H (CAT→CAC)	yohK	PF04712 family membrane protein YohK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/25);  total (14/25)

AAATCGCCGCGTCAATCCTGCCGAAATCAGTCACCACGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCATACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCT  >  NC_000913/2231371‑2231641                                                                                                                                         |                                                                                                                                       aaaTCGCCGCGTCAATCCTGCCGAAATCAGTCACCACGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCcacaca                                                                                                                                     <  2:74941/140‑1 (MQ=255) aaaTCGCCGCGTCAAGCCTGCCCAACTCAATCACCACCCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCcacaca                                                                                                                                     <  1:562069/140‑1 (MQ=255)            tCAATCCTGCCGAAATGGGTCACCACGCCTATTGCTATGGCGGTTGGCAGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAatgc                                                                                                                          <  2:687727/140‑1 (MQ=255)                      cGAAATCAGTCACCACGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTAtt                                                                                                                <  2:222275/140‑1 (MQ=255)                      cGAAATCAGTCACCACGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTAtt                                                                                                                <  2:113662/140‑1 (MQ=255)                      cGAAATCAGTCACCACGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTAtt                                                                                                                <  1:135303/140‑1 (MQ=255)                                     cGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGGATTTCCGCCATCAACGCAGGTTTCGGGGTTTTTGTCGGCCTCCTCCGCGCGGGGTTTTGCCCGCCAGTGCGTAATTCGGAGCGTTTTTGTACCAAAACTgcc                                                                                                 >  2:399212/1‑139 (MQ=255)                                     cGCCTATTGCAATGGCGGTTGGAGGAAGTCTTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTgcg                                                                                                 <  1:441877/140‑1 (MQ=255)                                       ccTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTgcgcg                                                                                               <  1:326995/140‑1 (MQ=255)                                       ccTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTgcgcg                                                                                               <  2:1116455/140‑1 (MQ=255)                                       ccTATTGCAATGGCGGTTGGCGCCAGTATTGCCGGCATTTCGGCAATCTGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTgcgcg                                                                                               <  2:426965/140‑1 (MQ=255)                                       ccTATTGCAATGGCGATTGGCGTCACTATGGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTgcgcg                                                                                               <  1:726809/140‑1 (MQ=255)                                       ccTATTGCAATGGCGATTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTgcgtg                                                                                               <  1:461431/140‑3 (MQ=255)                                              cAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTg                                                                                        <  2:1301102/140‑1 (MQ=255)                                              cAATGGCGGTTGGCGCCAGTATAGGCGGTATTCCGGCTATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTg                                                                                        <  1:702425/140‑1 (MQ=255)                                                           cggcAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTg                                                                           <  1:103183/140‑1 (MQ=255)                                                                  aTTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCa                                                                    <  1:1519798/140‑1 (MQ=255)                                                                           aTTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTTGCAATGGGGACTGACTCGCACGCCCTCgg                                                           >  1:522946/1‑140 (MQ=255)                                                                           aTTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGGATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATAGGGACTGCCTCGCACGCCCTCgg                                                           >  1:1272881/1‑140 (MQ=255)                                                                           aTTCCGGCAAACAGCGCAGTTTGCGGGATTTTCGTCGGCATCCTCGGCGCGGGATTTGGCCACACATTGCTTAATACGATGCGTATTTGTAGCAAAGCTGAGCGGGGTGTTGCAAATGGGACTGCCTCGCACcccccccg                                                           >  2:553000/1‑136 (MQ=255)                                                                           aTTCCCGCAATCAGCGCAGTTTGCGTGATTTTTGTCGGCATCCTCGGCGCGGGATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTAGCAAAACTGAGCGAGGGGTGGCAATGCGGGCTGCCTCGCACGCCCTCgg                                                           >  2:648062/1‑140 (MQ=255)                                                                           aTTCCCGCAAACACCGCAGTTTGCGTGATTTTCTTCGGCCTCCTCGGCGCCGTATTTTGCCACACATTTGTTAATGAGATGCGGATTCGTAACAAAGCTGGGCGAGGCTTGGCAATGTGGACAGTCTCGCCCGCCCCCgg                                                           >  2:470656/1‑140 (MQ=255)                                                                                 gCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGgc                                                     >  1:1325449/1‑140 (MQ=255)                                                                                    aTCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGgcgcg                                                  >  2:635840/1‑140 (MQ=255)                                                                                    aTCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGgcgcg                                                  >  2:1052368/1‑140 (MQ=255)                                                                                     tCAGCGCAGTTTGCGCGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCACGCGGCCTTGCATTGGCGACTGCCTAGCACGCCCTCGGTACGgcgcgc                                                 >  2:604686/1‑140 (MQ=255)                                                                                      cAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCt                                                >  1:202205/1‑140 (MQ=255)                                                                                         cgcAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTgcg                                             >  1:1402116/1‑140 (MQ=255)                                                                                          gcAGTTTGCGTGATTATCGTCGGCAGCCTCGGCGCAGGATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTgcgc                                            <  1:657167/140‑1 (MQ=255)                                                                                           cAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGcc                                           >  2:96680/1‑140 (MQ=255)                                                                                             gTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGa                                         <  2:1280788/140‑1 (MQ=255)                                                                                              tttGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAg                                        <  1:1394513/140‑1 (MQ=255)                                                                                               ttGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGGCTCGCACGCCCTTGGTAAGGCGCGCTGCGCCGAGc                                       >  2:644925/1‑140 (MQ=255)                                                                                                tGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCg                                          >  1:869812/1‑136 (MQ=255)                                                                                                tGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCg                                          <  2:869812/136‑1 (MQ=255)                                                                                                tGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCt                                      >  1:1389062/1‑140 (MQ=255)                                                                                                tGCGTGATTTTCGTCAGCATCCTCGTCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCt                                      <  1:648062/140‑1 (MQ=255)                                                                                                           cgtcgGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAgg                           <  1:1239908/140‑1 (MQ=255)                                                                                                            gtcgGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGa                          <  1:906626/140‑1 (MQ=255)                                                                                                             tcgGCATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGaa                         <  2:1389062/140‑1 (MQ=255)                                                                                                               ggCATCCTCGGCGCGATATTTGCCCGCACCTTGCTTATTGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAgg                       <  1:755391/140‑1 (MQ=255)                                                                                                                 cATCCTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTg                     >  2:1320320/1‑140 (MQ=255)                                                                                                                     cTCGGCGCGGTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCAtt                 >  2:1454096/1‑140 (MQ=255)                                                                                                                             ggTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAA‑GGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCt        <  1:1052836/140‑1 (MQ=255)                                                                                                                              gTATTTGGCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCt        <  1:635840/140‑1 (MQ=255)                                                                                                                                    ggCCACACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGGTGGATTAGCAGGAAGGTGCATTTAGTTCGCTAGCGCt  >  1:619315/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AAATCGCCGCGTCAATCCTGCCGAAATCAGTCACCACGCCTATTGCAATGGCGGTTGGCGGCAGTATTGGCGGTATTCCGGCAATCAGCGCAGTTTGCGTGATTTTCGTCGGCATCCTCGGCGCGGTATTTGGCCATACATTGCTTAATGCGATGCGTATTCGTACCAAAGCTGCGCGCGGTCTGGCAATGGGGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCT  >  NC_000913/2231371‑2231641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑