Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,336,818	A→G	V868A (GTT→GCT)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,336,818	0	A	G	100.0%	164.7 / NA	47	V868A (GTT→GCT)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/19);  total (28/19)

CTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAG  >  NC_000913/2336694‑2336951                                                                                                                             |                                                                                                                                      cTTTACCGTGCCCTAATACGACCAAAGCCCAAACTTTGCGGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAg                                                                                                                        <  2:662247/140‑1 (MQ=255)     aCCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGAt                                                                                                                    >  1:335793/1‑140 (MQ=255)      ccGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGAtt                                                                                                                   >  2:329819/1‑140 (MQ=255)           cccTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATc                                                                                                              >  1:889813/1‑140 (MQ=255)                     aCAAAAGCCCAGACTTTGCAGCCTGGACTTTTCGATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTcc                                                                                                    >  2:871402/1‑140 (MQ=255)                     aCAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTcc                                                                                                    >  2:254733/1‑140 (MQ=255)                      cAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCcg                                                                                                   >  1:137084/1‑140 (MQ=255)                      cAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCcg                                                                                                   >  1:1391821/1‑140 (MQ=255)                       aaaaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCcgc                                                                                                  <  2:1391821/140‑1 (MQ=255)                        aaaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGGGTCCACTGCCGGAGTGATTTCGTCGTCCCCTTCcgcg                                                                                                 >  1:426331/1‑140 (MQ=255)                        aaaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCcgcg                                                                                                 >  1:283782/1‑140 (MQ=255)                         aaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCgg                                                                                                >  1:1422963/1‑140 (MQ=255)                              cAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTg                                                                                           <  2:21298/140‑1 (MQ=255)                                     tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACt                                                                                            >  1:264766/1‑132 (MQ=255)                                     tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACt                                                                                            <  2:264766/132‑1 (MQ=255)                                     tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGa                                                                                    >  1:278620/1‑140 (MQ=255)                                     tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGa                                                                                    <  2:137084/140‑1 (MQ=255)                                     tGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGa                                                                                    <  2:1422963/140‑1 (MQ=255)                                             ggACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATcc                                                                            <  2:889813/140‑1 (MQ=255)                                                    tcaattcaaACAAGGGAGATAGTTCCCTTTTGGCATGAATAAGTAAAACTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCtt                                                                     <  2:467345/140‑1 (MQ=255)                                                    tcaattcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCACCTTCCGTGGCACTACCGTCGGTGGTATCCAGATCtt                                                                     >  1:634841/1‑140 (MQ=255)                                                             aCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCaa                                                            <  1:871402/140‑1 (MQ=255)                                                                      aTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAg                                                   <  2:335793/140‑1 (MQ=255)                                                                       tAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTGTCCGGACCTTCCTCGTCAACCGGTTCAGc                                                  >  1:504033/1‑140 (MQ=255)                                                                         gCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAcc                                                          <  1:183164/130‑1 (MQ=255)                                                                         gCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAcc                                                          >  2:183164/1‑130 (MQ=255)                                                                         gCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCaa                                                <  2:278620/140‑1 (MQ=255)                                                                         gCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCaa                                                >  2:339758/1‑140 (MQ=255)                                                                           tCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAaca                                              <  1:254733/140‑1 (MQ=255)                                                                             ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGGGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTGTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACg                                            >  2:877880/1‑140 (MQ=255)                                                                             ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAcc                                                          <  1:1099594/126‑1 (MQ=255)                                                                             ccTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAcc                                                          >  2:1099594/1‑126 (MQ=255)                                                                                   ggCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCGCTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAg                                      <  1:877880/140‑1 (MQ=255)                                                                                       tGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGAccc                                  >  1:785632/1‑140 (MQ=255)                                                                                       tGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGAccc                                  >  1:32368/1‑140 (MQ=255)                                                                                       tGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGAccc                                  >  1:1160121/1‑140 (MQ=255)                                                                                         aagaagTAAAATTATTCTTCTTCTGGCTCGTCGTCAGCGCCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCAc                                >  1:15570/1‑140 (MQ=255)                                                                                                      attCTTCTTCTGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCt                   >  1:1498284/1‑140 (MQ=255)                                                                                                         cttcttctGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTcc                >  1:1239091/1‑140 (MQ=255)                                                                                                          ttcttctGGCTCGTCGTCAGCGTCCGCTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCg               <  1:981872/140‑1 (MQ=255)                                                                                                          ttcttctGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCg               <  1:339758/140‑1 (MQ=255)                                                                                                          ttcttctGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCg               <  2:832314/140‑1 (MQ=255)                                                                                                            cttctGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCa             >  1:1329332/1‑140 (MQ=255)                                                                                                               ctGGCTCGTCGTCAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTa          <  2:785632/140‑1 (MQ=255)                                                                                                               ctGGCTCGTCGTCAGCGACCACCTCCGGAGCTATTACATAGTCCCCTTGCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTa          <  2:634841/140‑1 (MQ=255)                                                                                                                       tcgtcAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAg  >  2:1163369/1‑140 (MQ=255)                                                                                                                       tcgtcAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAg  >  1:1523232/1‑140 (MQ=255)                                                                                                                       tcgtcAGCGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAg  >  1:1122077/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                      CTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAG  >  NC_000913/2336694‑2336951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑