Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,357,848	A→G	pseudogene (33/186 nt)	ypaA →	protein YpaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,357,848	0	A	G	98.8%	262.6 / ‑4.6	83	pseudogene (33/186 nt)	ypaA	protein YpaA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (51/31);  total (51/32)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

GCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTG  >  NC_000913/2357721‑2357983                                                                                                                                |                                                                                                                                        gcATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATtggc                                                                                                                             <  2:244709/140‑1 (MQ=255)     gCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATtggctggc                                                                                                                         >  1:1054056/1‑140 (MQ=255)         gAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAg                                                                                                                       <  1:110792/138‑1 (MQ=255)         gAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAg                                                                                                                       >  2:110792/1‑138 (MQ=255)          aGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGTTGCAGCAGGAAGTGCATCAAATTGGCTGGCAGGaa                                                                                                                    >  2:596194/1‑140 (MQ=255)          aGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGaa                                                                                                                    >  2:1025087/1‑140 (MQ=255)          aGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGaa                                                                                                                    >  2:1055257/1‑140 (MQ=255)          aGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACAGCTGCGGGAGGAAGAGCATCAAATTGGCTGGCAGGaa                                                                                                                    >  1:413277/1‑140 (MQ=255)           gCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGTAAGGACATCAAATTGGCTGGCAGGAAg                                                                                                                   <  1:1007902/140‑1 (MQ=255)             aGGGCTTTGAACGTGAGATTGTTCTGGCGACTACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                                 <  2:532263/140‑1 (MQ=255)             aGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                                 <  2:1255652/140‑1 (MQ=255)             aGGGCTTTGAACGTGAGATTGTGCTAGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGACCGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                                 <  2:1084456/140‑1 (MQ=255)             aGGGCTTTGAACGTGAGATTGTCCTGGCGACTACCCAACCCACTGATGTTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                                 <  2:413277/140‑1 (MQ=25)               ggCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTaa                                                                                                               >  2:1074266/1‑140 (MQ=255)               ggCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTaa                                                                                                               >  1:242787/1‑140 (MQ=255)                   ttGAACGTGAGTTTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTa                                                                                                           >  1:1328991/1‑140 (MQ=255)                   ttGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCCGGAAGGGGATCAAAATGGCTGGCAGGAAGGTAAATTa                                                                                                           >  1:572918/1‑140 (MQ=255)                    tGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAAGGGCTGCGGTAGGGAGGGCATCAAATGGGCTGGCAGGAAGGTAAATTAg                                                                                                          >  2:424674/1‑140 (MQ=37)                    tGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATGGGCTGGCAGGAAGGTAAATTAg                                                                                                          >  1:632984/1‑140 (MQ=255)                          tGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGtt                                                                                                    >  2:1487782/1‑140 (MQ=255)                           gagaTTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGttt                                                                                                   >  1:228/1‑140 (MQ=255)                           gagaTTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGttt                                                                                                   >  2:1241545/1‑140 (MQ=255)                           gagaTTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGAATCAAATAGGCGGGTAGGAAGGTAAATTAGAAGGttt                                                                                                   >  1:433767/1‑140 (MQ=37)                                  tgCTGGCGACAACCCTACTCACTGACGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa                                                                                            <  1:526898/140‑1 (MQ=255)                                  tgCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa                                                                                            >  1:283308/1‑140 (MQ=255)                                      ggCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGGTGGCAGGGAGGCAAGTTAGAACGTTTGCATGAACAAg                                                                                        >  1:713503/1‑140 (MQ=25)                                       gCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCTTTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGc                                                                                       >  2:975752/1‑140 (MQ=255)                                       gCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGc                                                                                       <  1:1074266/140‑1 (MQ=255)                                       gCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCCGGCAGGAAGGTGAACTAGAAGGTTTGCATGAACAAGc                                                                                       >  1:588454/1‑140 (MQ=255)                                          aCAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCAt                                                                                    <  2:1230460/140‑1 (MQ=255)                                            aaCCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTa                                                                                  >  2:1072245/1‑140 (MQ=255)                                               ccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATAAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATAAAAAGGTTTGCATGAACAAGCCATTaaaa                                                                               <  2:328799/140‑1 (MQ=255)                                                cAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAAt                                                                              <  1:850177/140‑1 (MQ=255)                                                   cTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGc                                                                           <  1:1487782/140‑1 (MQ=255)                                                      aCTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCttt                                                                        >  1:508406/1‑140 (MQ=255)                                                        tGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGc                                                                                       <  1:1421695/123‑1 (MQ=255)                                                        tGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGc                                                                                       >  2:1421695/1‑123 (MQ=255)                                                         gATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTgcg                                                                     <  2:283308/140‑1 (MQ=255)                                                           tGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCa                                                                   <  1:223701/140‑1 (MQ=255)                                                              tGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGc                                                                <  2:508406/140‑1 (MQ=255)                                                              tGATATTCCGAACTGTAGTTAATAGGAGAGTATAACGACTATTGCTGACCGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGc                                                                <  2:713503/140‑1 (MQ=25)                                                                atatTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTg                                                              >  1:1158302/1‑140 (MQ=255)                                                                   ttCGGACCTGTCATTAAAAGGAGAGATTAATAAGTATGGCTGAACGGCTGCGTCAGGAAGGGCATCAAATTGGCTGGTAGGAAGATAAATTAGAGGGTTTCCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                                           <  2:1328991/140‑1 (MQ=25)                                                                   ttCCGAACTGTCATTAAAAGGAGAGCTTATTGACTATGGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                                           <  1:558920/140‑1 (MQ=255)                                                                   ttCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                                           <  2:228/140‑1 (MQ=255)                                                                   ttCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                                           <  2:242787/140‑1 (MQ=255)                                                                    tCCGAACTGTCATTACAAGGAGCGACTAATGACTATTCCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAAc                                                          <  1:424674/140‑1 (MQ=37)                                                                    tCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAAc                                                          <  1:838561/140‑1 (MQ=255)                                                                    tCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTCGCAGGAAGGTAAATTATAAGGTTTGGATGGACAGGCCATTAACATTGCTTTGCGCATGCTGGAAc                                                          >  2:573420/1‑140 (MQ=25)                                                                      cGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTCGCGCATGCTGGAACAg                                                        >  1:775817/1‑140 (MQ=255)                                                                        aaCTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGTTTTGCATGAACAAGACCTTAAAATTACTTTACGCATGCTCGAACAggg                                                      >  2:754143/1‑140 (MQ=21)                                                                        aaCTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAggg                                                      >  1:117175/1‑140 (MQ=255)                                                                        aaCTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAggg                                                      >  1:960064/1‑140 (MQ=255)                                                                        aaCTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAggg                                                      >  1:845068/1‑140 (MQ=255)                                                                           ccacaacaaaaGGAGAGATTAATGACTATTGCTGAAGGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCtt                                                   <  2:53683/133‑1 (MQ=255)                                                                            gTCATTAAAAGGAGAGAATAATCACTATTGCGGAAGGGCAGCGGCAGGGAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCttt                                                  <  2:632984/140‑1 (MQ=25)                                                                             tCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTg                                                 >  2:971912/1‑140 (MQ=255)                                                                                ttAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCACCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGAATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATc                                              <  1:1025087/140‑1 (MQ=255)                                                                                  aaaaGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAAGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGGAGGTTTGCATGAACAAGCCATAAAAGTTGGCTTGCGCAGGCTGTAACAGGGCTTTGATCGt                                            >  2:448155/1‑140 (MQ=21)                                                                                       gagagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATTAAATTGGCTGGCAGTAAGGTTAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGTGCATGCTGGAACAGGGCTTTGATCGTGACCa                                       >  1:377194/1‑140 (MQ=25)                                                                                       gagagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTTCTTTGCGCATGATGAAACAGGACTTTGATCGTGACCa                                       >  1:648001/1‑140 (MQ=25)                                                                                       gagagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                                       >  1:1199042/1‑140 (MQ=255)                                                                                       gagagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                                       >  1:1331031/1‑140 (MQ=255)                                                                                       gagagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                                       >  1:1133747/1‑140 (MQ=255)                                                                                         gagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAgg                                     >  1:1061245/1‑140 (MQ=255)                                                                                             taaaTGACTATTGCTTTATGGCTGCGGAGGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGATGGTTGGCGTGACCAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                                 <  2:377194/138‑1 (MQ=25)                                                                                                  gattATTGCTGACCGGCAGCGGAAGAAAGGCCATCAATTTGGCTGGCAGAAAGGTAATTAAGAGGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCgg                            <  2:1543469/137‑1 (MQ=25)                                                                                                     tATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCa                         <  2:845068/140‑1 (MQ=255)                                                                                                     tATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCa                         <  2:1061245/140‑1 (MQ=255)                                                                                                     tATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCa                         <  1:975752/140‑1 (MQ=255)                                                                                                     tATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCa                         <  2:117175/140‑1 (MQ=255)                                                                                                      aTTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGTAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAc                        >  1:969653/1‑140 (MQ=255)                                                                                                      aTTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGGTTTCATCGTGACCTGGTGCTCGCGGCCAc                        >  1:710176/1‑140 (MQ=255)                                                                                                      aTTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAc                        >  1:377300/1‑140 (MQ=255)                                                                                                      aTTGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAc                        <  2:1158302/140‑1 (MQ=255)                                                                                                       ttGCTGAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAcc                       <  2:960064/140‑1 (MQ=255)                                                                                                           tGAACGGCTGCGGCATGAAGGGTATCAAAGTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGc                   <  1:754143/140‑1 (MQ=255)                                                                                                            gAACGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCt                  >  2:958870/1‑140 (MQ=255)                                                                                                             aaCGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTa                 >  1:1122593/1‑140 (MQ=255)                                                                                                             aaCGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTa                 >  1:1174593/1‑140 (MQ=255)                                                                                                             aaCGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATACTGGAACAGGGCCTTGATCGTGACCCGGGGATCGCGGCCACCCAGCTa                 >  2:616434/1‑140 (MQ=37)                                                                                                               cGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCGTTGATCGTGACGAGGTGCTCGTGGCCACCCAGCTAAg               >  1:741715/1‑140 (MQ=255)                                                                                                               cGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCGTTGATCGGGACAAGGTGCTCGGGGCCACCCAGCTAAg               >  1:416651/1‑140 (MQ=37)                                                                                                               cGGCTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGCCTTCGATAGTGACCAGCTGCTGGCGGCCACCCAGCTAAg               >  2:473854/1‑140 (MQ=37)                                                                                                               cGGCTGCGGCAGGAAGCCCATAAAATTGGCACGGACGAAGGTAAATTAAAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAg               <  2:741715/140‑1 (MQ=25)                                                                                                                  cTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGa            >  2:300908/1‑140 (MQ=255)                                                                                                                  cTGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGAACAGTTGTTCGCGGACACCCAGCTAAGCGa            >  1:535357/1‑140 (MQ=37)                                                                                                                   tGCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGaa           <  2:1122593/140‑1 (MQ=255)                                                                                                                    gCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg          >  2:830653/1‑140 (MQ=255)                                                                                                                    gCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg          >  1:165799/1‑140 (MQ=255)                                                                                                                            aagggCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGGCACCCAGCTAAGCGAAGCCGATCTg  >  2:367432/1‑140 (MQ=255)                                                                                                                            aagggCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTGGATCGTGAGCAGGTGCTCGCGGCCAGCCAGGTAAGCGAAGCCGATCTg  >  2:437942/1‑140 (MQ=37)                                                                                                                                |                                                                                                                                        GCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTG  >  NC_000913/2357721‑2357983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑