Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,400,589	T→C	T323A (ACG→GCG)	nuoF ←	NADH:quinone oxidoreductase subunit F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,400,589	0	T	C	100.0%	274.5 / NA	78	T323A (ACG→GCG)	nuoF	NADH:quinone oxidoreductase subunit F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/33);  total (45/33)

AGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCAC  >  NC_000913/2400453‑2400720                                                                                                                                         |                                                                                                                                    aGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCAcc                                                                                                                                  >  2:12511/1‑140 (MQ=255) aGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCAcc                                                                                                                                  >  2:279943/1‑140 (MQ=255)      tttCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGAc                                                                                                                             <  1:359794/140‑1 (MQ=255)          aCGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCt                                                                                                                         <  2:1416050/140‑1 (MQ=255)             cTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGcc                                                                                                                      <  1:657423/140‑1 (MQ=255)               ccACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCg                                                                                                                    <  1:807656/140‑1 (MQ=255)                cACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGc                                                                                                                   <  2:1246936/140‑1 (MQ=255)                        aCCGTCGCGGCACGGGGTACACCAGGCGCCGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           <  1:740061/140‑1 (MQ=255)                        aCCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGcc                                                                                                                      >  2:1533987/1‑129 (MQ=255)                        aCCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGcc                                                                                                                      <  1:1533987/129‑1 (MQ=255)                        aCCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           >  2:1027845/1‑140 (MQ=255)                        aCCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           >  1:543375/1‑140 (MQ=255)                        aCCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           >  2:1458916/1‑140 (MQ=255)                        aCCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           >  1:1401367/1‑140 (MQ=255)                          cGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATa                                                                                                         >  1:1344062/1‑140 (MQ=255)                          cGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATa                                                                                                         >  2:1289633/1‑140 (MQ=255)                          cGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATa                                                                                                         >  1:285334/1‑140 (MQ=255)                               cgGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           <  2:1362261/133‑1 (MQ=255)                               cgGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGa                                                                                                           >  1:1362261/1‑133 (MQ=255)                                 gCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGa                                                                                                  <  2:284060/140‑1 (MQ=255)                                    cGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAAtt                                                                                               >  1:1455939/1‑140 (MQ=255)                                        gTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCAt                                                                                           <  2:174654/140‑1 (MQ=255)                                           cacCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCgg                                                                                        <  1:1005207/140‑1 (MQ=255)                                                 ccGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATc                                                                                  >  1:209937/1‑140 (MQ=255)                                                 ccGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACA‑CATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTcc                                                                                             <  2:1318888/128‑1 (MQ=255)                                                 ccGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACA‑CATGTTGATCTCATGGACAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTcc                                                                                             >  1:1318888/1‑128 (MQ=255)                                                   gCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCaa                                                                                >  1:50098/1‑140 (MQ=255)                                                          tCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTgcgc                                                                         >  2:878883/1‑140 (MQ=255)                                                            aCGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCtt                                                                       >  1:175946/1‑140 (MQ=255)                                                                 cGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTc                                                                  >  1:1095336/1‑140 (MQ=255)                                                                 cGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTc                                                                  >  1:382404/1‑140 (MQ=255)                                                                  gAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCa                                                                 <  1:279943/140‑1 (MQ=255)                                                                  gAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCa                                                                 <  1:12511/140‑1 (MQ=255)                                                                   aaaaaCTCTTCCAGGTTGCGCACCAGCGACAGCATATTGGTCTCATGGACAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAg                                                                <  2:543375/140‑1 (MQ=255)                                                                                 gTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCcg                                                  <  1:1458916/140‑1 (MQ=255)                                                                                   tACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGAAGAGCAAGGAGCGCTTACGTCTGGAAGTCAGTCCCcgcg                                                >  2:686106/1‑140 (MQ=255)                                                                                     cGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGcgcc                                              >  2:200268/1‑140 (MQ=255)                                                                                          cAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTg                                         >  2:68166/1‑140 (MQ=255)                                                                                           aGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTgg                                        >  2:1516726/1‑140 (MQ=255)                                                                                           aGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCCTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTgg                                        >  2:1153552/1‑140 (MQ=255)                                                                                             cGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCt                                      >  1:950248/1‑140 (MQ=255)                                                                                               acacCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGc                                    <  2:223421/140‑1 (MQ=255)                                                                                                cacCATGTTGATCTCCTGGTCAACCGCCATCGCCAGCTCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                   <  1:683748/140‑1 (MQ=255)                                                                                                cacCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                   <  2:209937/140‑1 (MQ=255)                                                                                                cacCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                   <  2:1052456/140‑1 (MQ=255)                                                                                                cacCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                   <  2:1095336/140‑1 (MQ=255)                                                                                                cacCATGTTGATCTCATGGTCAACCGCAATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                   <  1:958866/140‑1 (MQ=255)                                                                                                   cATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAgg                                <  2:1455939/140‑1 (MQ=255)                                                                                                     tGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCt                              >  2:1175986/1‑140 (MQ=255)                                                                                                        tGATCTCATGGTCAACCGCCATCGCTAGCGCCGCACCAAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTa                           <  1:686106/140‑1 (MQ=255)                                                                                                          aTCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaaa                         >  2:1206964/1‑140 (MQ=255)                                                                                                          aTCTCATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTaaa                         >  1:364936/1‑140 (MQ=255)                                                                                                           tctcATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCCTCGGTCAGGGAGCCTGCCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAt                        >  2:671341/1‑140 (MQ=255)                                                                                                            ctcATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAtt                       >  1:1170135/1‑140 (MQ=255)                                                                                                             tcATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCGGCGTCGGCTGGCTGCCAGGCTTTAAAttt                      >  1:562387/1‑140 (MQ=255)                                                                                                             tcATGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAttt                      >  2:1384345/1‑140 (MQ=255)                                                                                                               aTGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCCGGCTTTAAATTTCa                    <  2:950248/140‑1 (MQ=255)                                                                                                               aTGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCttt                            <  2:1411496/132‑1 (MQ=255)                                                                                                               aTGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCttt                            >  1:1411496/1‑132 (MQ=255)                                                                                                               aTGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                    <  1:200268/140‑1 (MQ=255)                                                                                                               aTGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGGCCCCGCGCCGCCAGGCTTACAGGCTTTAAATTTCa                    >  2:743244/1‑140 (MQ=255)                                                                                                                tGGTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCCGGCTGCCAGGCTTTAAATTTCAg                   >  1:340623/1‑140 (MQ=255)                                                                                                                 ggTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAttt                      <  2:806403/136‑1 (MQ=255)                                                                                                                 ggTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAttt                      >  1:806403/1‑136 (MQ=255)                                                                                                                 ggTCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGCCCCCGCGGCGCTTGCCTGCCAGGCTTTAAATTTCAGa                  >  1:685829/1‑140 (MQ=255)                                                                                                                   tCAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGAcc                <  2:364936/140‑1 (MQ=255)                                                                                                                    cAACCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCa               >  2:929337/1‑140 (MQ=255)                                                                                                                    cAACCGCCATCGCCACCGCCGCACCCAGAGGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCa               <  1:743244/140‑1 (MQ=255)                                                                                                                     aaCCGCCATCGCCAGCGGCGCACCCAGACGGCTGCGCGCATTACCGATGCTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt              <  2:513182/140‑1 (MQ=255)                                                                                                                     aaCCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt              >  1:292360/1‑140 (MQ=255)                                                                                                                      aCCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATc             >  2:1238416/1‑140 (MQ=255)                                                                                                                      aCCGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATc             >  2:285053/1‑140 (MQ=255)                                                                                                                       ccGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCa            <  2:1401367/140‑1 (MQ=255)                                                                                                                       ccGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCa            >  1:761641/1‑140 (MQ=255)                                                                                                                       ccGCCATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTCGCTACCCGGCTTTAAATTTCAGACCATCa            >  1:625973/1‑140 (MQ=255)                                                                                                                           cATCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCa        <  1:1516726/140‑1 (MQ=255)                                                                                                                             tCGCCAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATa      >  2:804936/1‑140 (MQ=255)                                                                                                                                ccAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATacca   <  1:1384345/140‑1 (MQ=255)                                                                                                                                 cAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATaccac  <  2:175946/140‑1 (MQ=255)                                                                                                                                 cAGCGCCGCACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATaccac  >  2:1168150/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    AGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCAC  >  NC_000913/2400453‑2400720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑