Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,407,653	A→G	E31E (GAA→GAG)	alaA →	glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,407,653	0	A	G	100.0%	316.7 / NA	88	E31E (GAA→GAG)	alaA	glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/49);  total (39/49)

CACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCC  >  NC_000913/2407519‑2407788                                                                                                                                       |                                                                                                                                        cACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTaa                                                                                                                                    <  2:1438204/140‑1 (MQ=255) cACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTaa                                                                                                                                    >  2:238631/1‑140 (MQ=255)  aCGGCAGGGAGTGGCGATAACATCAAAAAAGGTCATGATACATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAAc                                                                                                                                   <  2:587810/140‑1 (MQ=255)   cGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACa                                                                                                                                  <  1:1366594/140‑1 (MQ=255)   cGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACa                                                                                                                                  <  1:1210251/140‑1 (MQ=255)    ggCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCTGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACaa                                                                                                                                 <  2:1339487/140‑1 (MQ=255)      cAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAgg                                                                                                                               >  2:775476/1‑140 (MQ=255)      cAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTAAAAGAAGGAAAACGACTGGAATAAGAGGGTAACAAgg                                                                                                                               >  1:582094/1‑140 (MQ=255)         ggAGTGGTGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTac                                                                                                                            <  2:1288699/140‑1 (MQ=255)         ggAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTGCGGTGCTGAAAGGAGCAAAACGCCTAGAAGGAGAGGGTAACAAGGTac                                                                                                                            >  2:422630/1‑140 (MQ=255)         ggAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTac                                                                                                                            <  1:1397123/140‑1 (MQ=255)                cGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTactgaaact                                                                                                                     >  2:1266510/1‑140 (MQ=255)                    aaCAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAAc                                                                                                                 <  2:819626/140‑1 (MQ=255)                        gCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCg                                                                                                             <  2:889496/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTTATGTTCCCCATTGAAAACTCCCGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  2:546332/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGTAAATTCCGGCAAATTACAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  2:493136/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            >  1:1031423/1‑140 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            >  1:868069/1‑140 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  2:363864/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  1:903531/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  2:1097378/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  1:3486/140‑1 (MQ=255)                         cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCgg                                                                                                            <  2:883736/140‑1 (MQ=255)                            aaaaGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCaa                                                                                                         <  1:238631/140‑1 (MQ=255)                                 gTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAg                                                                                                    <  1:775476/140‑1 (MQ=255)                                   cAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGcc                                                                                                  <  2:985911/140‑1 (MQ=255)                                      gATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAA‑‑CAGCCCCGtt                                                                                             <  2:859930/140‑1 (MQ=255)                                           aTGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGCGGGTAACAAGGTACTGAAACTGAACTTCGGCAACCCAGCCCCGTTCgg                                                                                          >  2:654202/1‑140 (MQ=255)                                                    aTTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGAcgc                                                                                 <  1:1490615/140‑1 (MQ=255)                                                     ttGAAAAATCCAGCACATTAGATAATGTCTGTTATGAGATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGAcgcg                                                                                <  1:562384/140‑1 (MQ=255)                                                      tGAAAAATCCAGCAAATTAGAGAATTTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACgcgc                                                                               <  1:705719/140‑1 (MQ=255)                                                      tGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACgcgc                                                                               <  2:524201/140‑1 (MQ=255)                                                             tCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGa                                                                        >  2:764369/1‑140 (MQ=255)                                                                  cAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATcc                                                                   >  2:1146725/1‑140 (MQ=255)                                                                      ttAGAGAATGTCTGTCATGACACCCCTGGTCCGGTGCAGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGt                                                               <  1:422630/140‑1 (MQ=255)                                                                           gaATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACg                                                          <  2:1271608/140‑1 (MQ=255)                                                                              tGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGTCGCTCCAGATGAACTCCTCGTTGACGTGa                                                       >  1:537004/1‑140 (MQ=255)                                                                              tGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGa                                                       >  1:1350568/1‑140 (MQ=255)                                                                               gTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGAt                                                      >  2:1400920/1‑140 (MQ=255)                                                                                   gTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGc                                                  <  1:40862/140‑1 (MQ=255)                                                                                    ttATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCa                                                 >  2:7364/1‑140 (MQ=255)                                                                                      aTGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATa                                                     <  2:947628/134‑1 (MQ=255)                                                                                      aTGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATa                                                     >  1:947628/1‑134 (MQ=255)                                                                                      aTGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAAc                                               >  1:194531/1‑140 (MQ=255)                                                                                        gACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACTCGCCAGAGGAAGTCCTCGTTGACGTGATACGCAACCt                                             >  1:645869/1‑140 (MQ=255)                                                                                          cATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGAc                                                           >  1:1330324/1‑124 (MQ=255)                                                                                          cATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGAc                                                           <  2:1330324/124‑1 (MQ=255)                                                                                           aTCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGcc                                          <  2:1520951/140‑1 (MQ=255)                                                                                            tCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCt                                         >  1:1086746/1‑140 (MQ=255)                                                                                            tCCGTGGTCCGGTACTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGCTGACGTGATACGCAACCTGCCt                                         >  1:730929/1‑140 (MQ=255)                                                                                             ccGTGGTCCGGTGCTCAAAGAAACACAACGCCTGGAATAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTa                                        <  1:649094/140‑1 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTAc                                       >  2:1503775/1‑140 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTAc                                       >  1:104310/1‑140 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTAc                                       >  2:1314646/1‑140 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTAc                                       >  1:1546823/1‑140 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTAc                                       <  1:1503775/140‑1 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTAc                                       <  1:143757/140‑1 (MQ=255)                                                                                              cGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGAGGGATTCCTCGTTGACGTATTACGCAACCTGCCTAc                                       >  2:701823/1‑140 (MQ=255)                                                                                                tGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCg                                     <  2:1546823/140‑1 (MQ=255)                                                                                                tGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCg                                     >  1:1311972/1‑140 (MQ=255)                                                                                                tGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCCCGTTGATGTGATAGGCAACCTGCCTACCg                                     >  1:528804/1‑140 (MQ=255)                                                                                                tGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAAACCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCg                                     >  1:1502489/1‑140 (MQ=255)                                                                                                 ggTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTa                                        >  2:284907/1‑136 (MQ=255)                                                                                                 ggTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTa                                        <  1:284907/136‑1 (MQ=255)                                                                                                 ggTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGc                                    >  2:980897/1‑140 (MQ=255)                                                                                                   tCCGGTGCTGAAAGAAGGAAAAAGCCTGGAAGAAGAGGGTAACAAGGTTCTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTc                                  <  2:537004/140‑1 (MQ=255)                                                                                                    ccGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCa                                 <  2:1502489/140‑1 (MQ=255)                                                                                                       gTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCGTCGTTGAGGTGGTAGGCAACCTGCCTACCGCTCAAgg                              >  1:451338/1‑140 (MQ=255)                                                                                                       gTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCAAGATGAAAACCTCGTTTACGTGATAGGCAACCTGCCTACCGCTCAAgg                              >  2:410600/1‑140 (MQ=255)                                                                                                       gTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGCAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAgg                              >  2:935321/1‑140 (MQ=255)                                                                                                        tGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAggg                             >  1:1305522/1‑140 (MQ=255)                                                                                                        tGCTGAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAggg                             >  2:312626/1‑140 (MQ=255)                                                                                                            gAAAGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTAtt                         <  2:1311972/140‑1 (MQ=255)                                                                                                             aaaGAAGCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTg                        >  2:1484723/1‑140 (MQ=255)                                                                                                              aagaagCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGc                       <  2:868069/140‑1 (MQ=255)                                                                                                                  agCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGAtt                   >  2:1117701/1‑140 (MQ=255)                                                                                                                  agCAAAACGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACCGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGAtt                   >  2:1015539/1‑140 (MQ=255)                                                                                                                     aaaaCGCCTGGAAGAAGCGGGTTACAAGGTAGTGACACTCAACATCGGTAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                <  2:418223/140‑1 (MQ=255)                                                                                                                     aaaaCGCCTGGAAGAAGAGGGTCACAAGGCACTGAAATTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                <  1:701823/140‑1 (MQ=255)                                                                                                                     aaaaCGCCTGGAAGAAGAGGGTAACCAGGTACTGAAATTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                <  1:613314/140‑1 (MQ=255)                                                                                                                     aaaaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                <  1:1484723/140‑1 (MQ=255)                                                                                                                     aaaaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                <  2:1086746/140‑1 (MQ=255)                                                                                                                     aaaaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                <  1:1275344/140‑1 (MQ=255)                                                                                                                       aaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCTGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa              <  2:939088/140‑1 (MQ=255)                                                                                                                       aaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa              <  1:240181/140‑1 (MQ=255)                                                                                                                       aaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa              <  2:194531/140‑1 (MQ=255)                                                                                                                       aaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa              >  1:1242742/1‑140 (MQ=255)                                                                                                                       aaCGCCTGGAAGAAGAGGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCaaa              <  1:1146725/140‑1 (MQ=255)                                                                                                                                   aagaggGTAACAAGGTATTGAATCTGAACATTGGCCACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTcc  <  2:645869/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCC  >  NC_000913/2407519‑2407788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑