Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,407	T→C	N515S (AAC→AGC)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,407	0	T	C	100.0%	263.7 / NA	74	N515S (AAC→AGC)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/36);  total (38/36)

GTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCATTATTAAAAA  >  NC_000913/2490273‑2490539                                                                                                                                       |                                                                                                                                      gTTTTGGGTTAAGTTTGTTAATCTGGCCACTTTCACTTCCTGCTGCAGCGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAg                                                                                                                                  <  2:443301/140‑1 (MQ=255)   tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGcc                                                                                                                                <  1:307936/140‑1 (MQ=255)                 ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGGGGTGACACTATAGGCAACGCCACGAAACg                                                                                                                  >  1:437801/1‑140 (MQ=255)                 ggTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACg                                                                                                                  >  2:1510556/1‑140 (MQ=255)                  gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGc                                                                                                                 <  2:43284/140‑1 (MQ=255)                        tGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCAt                                                                                                           >  1:359457/1‑140 (MQ=255)                          gCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTa                                                                                                         <  1:170791/140‑1 (MQ=255)                          gCCACTTTCAGTTCCTGCTGCACGGTCGAGGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTATCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTa                                                                                                         <  1:695140/140‑1 (MQ=255)                          gCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTa                                                                                                         <  1:1510556/140‑1 (MQ=255)                            cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAAt                                                                                                       <  2:1334209/140‑1 (MQ=255)                                 tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc                                                                                                  <  1:122310/140‑1 (MQ=255)                                   aGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAt                                                                                                <  2:371611/140‑1 (MQ=255)                                         tgctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTg                                                                                          >  2:61451/1‑140 (MQ=255)                                          gctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGc                                                                                         >  1:951698/1‑140 (MQ=255)                                           ctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCa                                                                                        <  2:65014/140‑1 (MQ=255)                                             gcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATg                                                                                      <  2:288341/140‑1 (MQ=255)                                                gggTCGATGACCACATTCATAATGGTCGGTTCGCGCGACGGAATACCGCTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTg                                                                                   <  2:437801/140‑1 (MQ=255)                                                    cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAAc                                                                               <  1:1266620/140‑1 (MQ=255)                                                      aTGGCCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGAAGAAGTTCATCTGTCGTGGTCACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTTTCATACCTTGCATGGTGCAACAg                                                                             <  2:1411821/140‑1 (MQ=255)                                                        gACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGAt                                                                           <  2:839378/140‑1 (MQ=255)                                                          ccACATTAATAATGGTCGGTTTCCGCGACTGAATAGCGGTGGTTAAAGTATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATcc                                                                         <  2:581529/140‑1 (MQ=255)                                                             caTTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGCAACGCATCGATTTATTTGTCATACCTTGCATGGTGCAACAGATCCGtt                                                                      >  2:589305/1‑140 (MQ=255)                                                             caTTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATgg                                                                                     <  2:875008/125‑1 (MQ=255)                                                             caTTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATgg                                                                                     >  1:875008/1‑125 (MQ=255)                                                               ttAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTgg                                                                    <  2:359457/140‑1 (MQ=255)                                                                  ataatGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGa                                                                 <  2:1404239/140‑1 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGCAGCTCACCTTTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCAATAATTTGTGATAGCTTGCATGGTGCGACAGATCCGTTGGTGATGGTGc                                                           >  2:398789/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCGCGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:1269604/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATGCCTTGCATGGCGCCACAGATCCGTTGGTGATGGTGc                                                           >  1:560894/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  1:807198/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  1:1511078/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  1:238683/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:122617/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:1082773/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:1047736/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:1032961/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:1018818/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  1:830667/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCACTAATTTGTGATAACTTGCATGGTGCGACAGATCCGTTGGTGATGGTGc                                                           >  2:426574/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCAATAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGc                                                           >  2:630774/1‑140 (MQ=255)                                                                        gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCAATAATTTGTAATATCCTGAATGGCGCGACAGACCCGTTGGCGATGGTGc                                                           >  2:746008/1‑140 (MQ=255)                                                                          cGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCAc                                                         <  1:61451/140‑1 (MQ=255)                                                                          cGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCAc                                                         >  2:1446333/1‑140 (MQ=255)                                                                               tGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAgcg                                                    <  2:830667/140‑1 (MQ=255)                                                                                  gcgACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCa                                                 <  2:1541567/140‑1 (MQ=255)                                                                                  gcgACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGAGACACTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATCCCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCa                                                 <  2:322260/140‑1 (MQ=255)                                                                                    gACTGAATACCGGTGTTTAAAGGATTACGCAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACt                                               <  1:746008/140‑1 (MQ=255)                                                                                    gACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACt                                               <  1:1047736/140‑1 (MQ=255)                                                                                    agacagaTACCGGTGCTTAAAGGATTACGCAGTTCATATGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACt                                               <  1:469891/134‑1 (MQ=255)                                                                                                gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCAGCAGCGCCACt                                               <  2:146736/128‑1 (MQ=255)                                                                                                gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACt                                               >  1:1426216/1‑128 (MQ=255)                                                                                                gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACt                                               >  1:146736/1‑128 (MQ=255)                                                                                                gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACt                                               <  2:1426216/128‑1 (MQ=255)                                                                                                gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAc‑c                                  >  1:177180/1‑140 (MQ=255)                                                                                                gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTCGGTGCTGGTGCAGCAGCGCCACTGAGCTCAACAc‑c                                  >  2:718879/1‑140 (MQ=255)                                                                                                  ggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGt                                <  1:329755/140‑1 (MQ=255)                                                                                                  ggtTAAAGCATGACGAACTTCATCTGTCGTGCTGACGCTATAGCCAACTCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTACAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGt                                <  2:623523/140‑1 (MQ=255)                                                                                                   gtTAAAGCATGACGAGGTTCATCTGGCGTGGTGACGCGATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGtc                               <  1:589305/140‑1 (MQ=255)                                                                                                   gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGtc                               >  1:319066/1‑140 (MQ=255)                                                                                                     tAAAGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTCGTGGTGGCGCACCAGCACCACTGAGATCAACAc‑ctgtct                             >  2:469891/1‑135 (MQ=255)                                                                                                     tAAAGCATGACGAAGTTCATCTCTCCTGGTGACGCTACAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGtctc                             <  1:478702/140‑1 (MQ=255)                                                                                                       aaGCATGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTctcct                           <  1:1082773/140‑1 (MQ=255)                                                                                                           aTGACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGt                       >  2:1291354/1‑140 (MQ=255)                                                                                                             gACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGGACCAGCGACACAGAGATCAACCC‑CGTCTCCTCTGTAg                     >  1:538999/1‑140 (MQ=255)                                                                                                             gACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGtctc                             >  1:916277/1‑132 (MQ=255)                                                                                                             gACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGtctc                             <  2:916277/132‑1 (MQ=255)                                                                                                             gACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGt                                >  2:344712/1‑129 (MQ=255)                                                                                                             gACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGt                                <  1:344712/129‑1 (MQ=255)                                                                                                             gACGAAGTTCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTACAACAGATCCGTTGGTGATGGTGAACCAGCGACACTGAAATCAACCC‑CGTCTCCTCTGTAg                     >  1:623523/1‑140 (MQ=255)                                                                                                                    ttCATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATgccgc              <  1:1032961/140‑1 (MQ=255)                                                                                                                      cATTTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCa            <  2:74943/140‑1 (MQ=255)                                                                                                                      cATCTGTCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCa            <  2:951698/140‑1 (MQ=255)                                                                                                                           gTCGTGGTGACGCTATAGCCAAAGCCACGCAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCattatt       <  1:426574/140‑1 (MQ=255)                                                                                                                            tCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCAttatta      >  2:1031767/1‑140 (MQ=255)                                                                                                                            tCGTGGTGACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTACAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCAttatta      >  1:991131/1‑140 (MQ=255)                                                                                                                              gtggtgACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCATTATTaaa    >  1:278892/1‑140 (MQ=255)                                                                                                                              gtggtgACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGACATC‑ACACGCGTCCCCTTTGTACATCCCGCCTTTATaaaa    >  2:689553/1‑140 (MQ=255)                                                                                                                                ggtgACGCTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCATTATTaaaaa  >  1:1208497/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACAC‑CGTCTCCTCTGTAGATGCCGCCATTATTAAAAA  >  NC_000913/2490273‑2490539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑