Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	395,146	A→G	V381A (GTT→GCT)	ampH ←	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	395,146	0	A	G	100.0%	295.0 / NA	82	V381A (GTT→GCT)	ampH	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/42);  total (40/42)

CGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATAT  >  NC_000913/395010‑395278                                                                                                                                         |                                                                                                                                     cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCa                                                                                                                                   >  1:1326423/1‑140 (MQ=255)  gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCaa                                                                                                                                  <  1:790434/140‑1 (MQ=255)  gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCaa                                                                                                                                  <  2:62779/140‑1 (MQ=255)  gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCaa                                                                                                                                  <  1:307719/140‑1 (MQ=255)  gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCaa                                                                                                                                  <  2:1029067/140‑1 (MQ=255)  gTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCaa                                                                                                                                  <  2:1425065/140‑1 (MQ=255)  gTACACGCATATCGCGTGCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCCTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCaa                                                                                                                                  <  2:507828/140‑1 (MQ=255)      acGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCATAGTATTAATCAGGACGCGGGGATAGCCAACGGt                                                                                                                              >  2:119397/1‑140 (MQ=255)      acGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGt                                                                                                                              >  1:1521105/1‑140 (MQ=255)       cGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGtt                                                                                                                             <  1:317433/140‑1 (MQ=255)       cGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGtt                                                                                                                             >  1:1191032/1‑140 (MQ=255)          atatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTAt                                                                                                                          <  2:282791/140‑1 (MQ=255)          atatCGCGTTCTGTCATCGCAGCAGCTCTAGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTAt                                                                                                                          <  1:513029/140‑1 (MQ=255)           tatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTAtt                                                                                                                         <  2:1326423/140‑1 (MQ=255)           tatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTAtt                                                                                                                         <  2:846377/140‑1 (MQ=255)           tatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTAtt                                                                                                                         >  2:997177/1‑140 (MQ=255)           tatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTCAtt                                                                                                                         >  1:355819/1‑140 (MQ=255)            atCGCGTTCTATCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTGTGCCTTCACAGTATTAGTCAGGACGAGGGGTTAGCCAACGGTTTATTc                                                                                                                        >  2:414943/1‑140 (MQ=255)                cgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGc                                                                                                                    >  2:230126/1‑140 (MQ=255)                cgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGc                                                                                                                    >  2:1043145/1‑140 (MQ=255)                  ttCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTGCAGAGTATCAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCtt                                                                                                                  >  2:594203/1‑140 (MQ=255)                    cTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAg                                                                                                                >  2:1271361/1‑140 (MQ=255)                      gtcgtcGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCATAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCt                                                                                                              <  1:119397/140‑1 (MQ=255)                      gtcgtcGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCt                                                                                                              <  2:1284244/140‑1 (MQ=255)                        cgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCg                                                                                                            <  1:1043145/140‑1 (MQ=255)                             cAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAGCTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCAc                                                                                                       <  2:1160859/140‑1 (MQ=255)                             cAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCAc                                                                                                       <  2:40347/140‑1 (MQ=255)                             cAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCAc                                                                                                       <  1:997177/140‑1 (MQ=255)                                aaGTTCTCTCTGCTTTAGGTAAAGGCGGCTATATCGAAACGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAg                                                                                                    <  2:690498/140‑1 (MQ=255)                                aaGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAg                                                                                                    <  1:230126/140‑1 (MQ=255)                                      tcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCAtt                                                                                              <  2:1191032/140‑1 (MQ=255)                                        gctgctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAAGGGTTTATACCCGCTTAGCTCGATCACCAGGTCATTGa                                                                                            >  1:427494/1‑140 (MQ=255)                                           gctTTGCGTAAAGGCGGCTATACCGAAATGATTAACGGCTAACTGGTCCCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGc                                                                                         <  2:427494/140‑1 (MQ=255)                                            ctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGcc                                                                                        <  1:1077937/140‑1 (MQ=255)                                              ttGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTCATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCAt                                                                                      <  2:355819/140‑1 (MQ=255)                                                                   gAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGcgcg                                                                 >  2:562126/1‑140 (MQ=255)                                                                      aTGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCa                                                              >  2:1502985/1‑140 (MQ=255)                                                                       tGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                                                             <  1:1502985/140‑1 (MQ=255)                                                                       tGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                                                             <  1:562126/140‑1 (MQ=255)                                                                         aaTAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                                                           >  1:383165/1‑140 (MQ=255)                                                                         aaTAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                                                           >  2:1096780/1‑140 (MQ=255)                                                                           tAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                                                           <  1:210775/138‑1 (MQ=255)                                                                           tAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                                                           >  2:210775/1‑138 (MQ=255)                                                                               ggCAAACTGGTCGCTATCAACCATTTGCCTTCAGAGTCTTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAAc                                                     <  1:537529/140‑1 (MQ=255)                                                                                   aaCTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCGATCCCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt                                                 >  2:617063/1‑140 (MQ=255)                                                                                   aaCTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt                                                 >  1:79820/1‑140 (MQ=255)                                                                                   aaCTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt                                                 >  1:178735/1‑140 (MQ=255)                                                                                   aaCTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGt                                                 >  2:965362/1‑140 (MQ=255)                                                                                     cTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                                                             >  1:861610/1‑126 (MQ=255)                                                                                     cTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                                                             <  2:861610/126‑1 (MQ=255)                                                                                        gTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACTGCGAACGAGTGacca                                            >  1:493393/1‑140 (MQ=255)                                                                                          cGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGaccacc                                          >  2:941088/1‑140 (MQ=255)                                                                                             tATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACcaca                                       <  2:1521105/140‑1 (MQ=255)                                                                                              aTCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACaa                                      >  1:150784/1‑140 (MQ=255)                                                                                                 aaCCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAAcg                                   <  1:1271361/140‑1 (MQ=255)                                                                                                       ttGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAAcgc                                  >  1:1016232/1‑135 (MQ=255)                                                                                                       ttGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAAcgc                                  <  2:1016232/135‑1 (MQ=255)                                                                                                       ttGCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCaa                             >  1:354356/1‑140 (MQ=255)                                                                                                         gCCTTCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAata                           >  2:1170246/1‑140 (MQ=255)                                                                                                            ttCAGAGTATTAATCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCATCGGCGAAAGAGTGAGCACCACTAACGCGCCAATAttc                        >  1:640541/1‑140 (MQ=255)                                                                                                                       aaTCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGat             <  1:965362/140‑1 (MQ=255)                                                                                                                       aaTCAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGat             >  1:1078404/1‑140 (MQ=255)                                                                                                                         tCAGGACGCGGGGATAGCCAACGGTCTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGatca           <  1:565470/140‑1 (MQ=255)                                                                                                                          cAGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGatcat          <  1:941088/140‑1 (MQ=255)                                                                                                                           aGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGatcatc         >  2:1254457/1‑140 (MQ=255)                                                                                                                           aGGACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGatcatc         <  1:1096780/140‑1 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCTCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCGCCACAAACGCGTCAATATTCTGCTGCGGGATCATCGc       >  1:648237/1‑140 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGCGTGACCACCACAAACGCGCCAATCTTCTTCTGCGGGATCATCGc       >  1:25050/1‑140 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGAGCACGACATACGCGCCACTATTCTTCTGCGGGATCATCGc       >  2:565470/1‑140 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATAtt                         <  1:13440/122‑1 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATAtt                         >  2:13440/1‑122 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGc       >  2:910942/1‑140 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGc       >  1:1120643/1‑140 (MQ=255)                                                                                                                             gACGCGGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAAAGCGCCAATATTCTTCTGCGGGATCATCGc       >  1:512189/1‑140 (MQ=255)                                                                                                                               cgcgGGGATAGCCAACGGTTTATTCCGGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGatcat          <  2:245924/135‑1 (MQ=255)                                                                                                                               cgcgGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGatcat          >  1:245924/1‑135 (MQ=255)                                                                                                                                gcgGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCat    <  2:150784/140‑1 (MQ=255)                                                                                                                                 cgGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCata   <  1:1170246/140‑1 (MQ=255)                                                                                                                                 cgGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCata   <  2:383165/140‑1 (MQ=255)                                                                                                                                 cgGGGATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCata   <  2:1078404/140‑1 (MQ=255)                                                                                                                                  ggggATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGat             >  1:973473/1‑129 (MQ=255)                                                                                                                                  ggggATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGat             <  2:973473/129‑1 (MQ=255)                                                                                                                                  ggggATAGCCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCatat  <  2:25050/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATAT  >  NC_000913/395010‑395278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑