Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,533,577	T→C	intergenic (+197/‑187)	cysK → / → ptsH	O‑acetylserine sulfhydrylase A/phosphocarrier protein HPr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,533,577	0	T	C	100.0%	303.6 / NA	85	intergenic (+197/‑187)	cysK/ptsH	O‑acetylserine sulfhydrylase A/phosphocarrier protein HPr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/46);  total (39/46)

TCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGA  >  NC_000913/2533449‑2533707                                                                                                                                 |                                                                                                                                   tCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGc                                                                                                                         >  1:1521714/1‑140 (MQ=255) tCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGc                                                                                                                         >  2:831490/1‑140 (MQ=255) tCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGc                                                                                                                         >  1:1266184/1‑140 (MQ=255) tCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGc                                                                                                                         >  1:92034/1‑140 (MQ=255)  gacagctcctGGCATGGATTCATCCTGTCTGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCa                                                                                                                        <  1:611459/135‑1 (MQ=255)  cGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCa                                                                                                                        <  1:1520976/140‑1 (MQ=255)   gCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGCCAt                                                                                                                       >  1:1509093/1‑140 (MQ=255)    ccccTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATa                                                                                                                      <  1:387647/140‑1 (MQ=255)    ccccTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATa                                                                                                                      <  1:224415/140‑1 (MQ=255)    ccccTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATa                                                                                                                      <  1:887595/140‑1 (MQ=255)        tcctGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCAGAATCGATATTAAGATCTGGTTCAAGTCTCCCTTTAGCGGCATAATGt                                                                                                                  >  2:536273/1‑140 (MQ=255)           tGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTa                                                                                                               <  2:206594/140‑1 (MQ=255)             gCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAAt                                                                                                             <  2:92034/140‑1 (MQ=255)               aTTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGa                                                                                                           <  2:331575/140‑1 (MQ=255)                 tGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTATGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACg                                                                                                         >  2:59527/1‑140 (MQ=255)                  gATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTAAATTCTCCCTTTGGCGGAATAATGTTTAAtctgtc                                                                                                        >  2:611459/1‑135 (MQ=255)                   aTTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTa                                                                                                       >  2:1006815/1‑140 (MQ=255)                      cAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTaa                                                                                                              <  2:255579/130‑1 (MQ=255)                      cAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTaa                                                                                                              >  1:255579/1‑130 (MQ=255)                       aGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAATCTCCCTTTAGGGGAATAATGTTTAATGACGTACGaa                                                                                                   >  1:507536/1‑140 (MQ=255)                             tCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCa                                                                                             >  2:1438476/1‑140 (MQ=255)                              cGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAg                                                                                            <  1:831490/140‑1 (MQ=255)                              cGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAg                                                                                            <  1:1059692/140‑1 (MQ=255)                                  aCTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGt                                                                                        <  2:1509093/140‑1 (MQ=255)                                      gTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCa                                                                                             >  2:1120614/1‑131 (MQ=255)                                      gTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCa                                                                                             <  1:1120614/131‑1 (MQ=255)                                       tATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCCTAATGTTCAATAACATACGAAGCGTCAGCGGTCAaca                                                                                   >  1:556102/1‑140 (MQ=255)                                       tATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAaca                                                                                   >  2:185182/1‑140 (MQ=255)                                            aaCCAGACTAATTATTTTGATGCGCGAAATTGATCTTTAAAGGAAAAGTCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCCCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGc                                                                              <  2:507536/140‑1 (MQ=255)                                            aaCCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGc                                                                              <  1:1006815/140‑1 (MQ=255)                                            aaCCAGACTAATTATGTTGATGCGCGAAATTAATCGTCACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGc                                                                              <  1:477121/140‑1 (MQ=255)                                             aCCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAACGGTCAACACCCGcc                                                                             >  1:616280/1‑140 (MQ=255)                                                aGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGc                                                                          >  1:1000217/1‑140 (MQ=255)                                                aGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGc                                                                          >  2:325450/1‑140 (MQ=255)                                                 gACTAATTCTTTTGATGGGCGACATTAATCTTTACTGGATAAGCCAAATCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCa                                                                         <  2:743094/140‑1 (MQ=255)                                                 gACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGGATAATGTTCAATGACGGACGAAACGACAGCGGTCAACACCCGCCAGCa                                                                         >  2:625747/1‑140 (MQ=255)                                                 gACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTAGGAATCGTCAGCGGCCAACACCCGCCAGCa                                                                         >  1:743094/1‑140 (MQ=255)                                                 gACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCa                                                                         >  2:1067455/1‑140 (MQ=255)                                                 gACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCa                                                                         <  1:119997/140‑1 (MQ=255)                                                 gACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTCAATGACGCACGAAACGCCAGCGGTCAACACCCGCCAGCa                                                                         >  2:550629/1‑140 (MQ=255)                                                  aCTAATTATTTTGATGCGGGAAATTAATCGTTAAAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCaa                                                                        <  1:910600/140‑1 (MQ=255)                                                   cTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCa                                                                         >  2:1343598/1‑138 (MQ=255)                                                   cTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAAt                                                                       <  1:185182/140‑1 (MQ=255)                                                   cTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAAt                                                                       >  2:1431236/1‑140 (MQ=255)                                                   cTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTAAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCa                                                                         <  1:1343598/138‑1 (MQ=255)                                                    tAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATACTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGAGGTCAACACCCGCCAGCAATg                                                                      <  1:1180388/140‑1 (MQ=255)                                                      atttttTTGAGGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGTTTCAATTCCCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGa                                                                    <  2:1114006/140‑1 (MQ=255)                                                      attattTTGATGCGCGAAATTAATCGTTACAGGAACAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACCCCCGCCAGCAATGGa                                                                    <  2:658752/140‑1 (MQ=255)                                                      attattTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGa                                                                    <  1:859610/140‑1 (MQ=255)                                                      attattTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGa                                                                    <  1:834066/140‑1 (MQ=255)                                                      attattTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGa                                                                    <  1:1424163/140‑1 (MQ=255)                                                            ttGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGATACGTCAGCGGTCAAGACCCGCAAGCAATGGACTGTAt                                                              >  1:700434/1‑140 (MQ=255)                                                              gATGCGCAAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTg                                                            <  2:1443130/140‑1 (MQ=255)                                                               aTGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTgc                                                           >  1:1266238/1‑140 (MQ=255)                                                                tGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAAt                                                                       >  1:1472895/1‑127 (MQ=255)                                                                tGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAAt                                                                       <  2:1472895/127‑1 (MQ=255)                                                                       aaTTAATCGTTACAGGAAAAGCGAACGCTCAATCGATGTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCg                                                   <  1:550629/140‑1 (MQ=255)                                                                              cGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcg                                            >  1:803649/1‑140 (MQ=255)                                                                               gTTACAGGAAAAGCCAAAGCTGTATCGATTTTATGTTTTGGTTCAATTATCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcgc                                           <  2:616280/140‑1 (MQ=255)                                                                                 tACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcgcgt                                         <  2:1266238/140‑1 (MQ=255)                                                                                 tACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcgcgt                                         >  2:236061/1‑140 (MQ=255)                                                                                 tACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcgcgt                                         >  2:797045/1‑140 (MQ=255)                                                                                 tACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcgcgt                                         <  2:262314/140‑1 (MQ=255)                                                                                 tACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGACAGCAATGGACTGTATTGCGCGCTTCGTgcgtcgcgt                                         >  1:529214/1‑140 (MQ=255)                                                                                  aCAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTgcgtcgcgtc                                        >  1:106258/1‑140 (MQ=255)                                                                                   cAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTTAACACCCGGCAGAAATAGACTGTATGGCGCGCTTCGTGCGTCGCGTCt                                       >  2:460636/1‑140 (MQ=255)                                                                                   cAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCt                                       >  1:17891/1‑140 (MQ=255)                                                                                             cAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATG‑TTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTg                            <  1:854630/140‑1 (MQ=255)                                                                                              aaaGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTg                            <  1:797045/140‑1 (MQ=255)                                                                                                agttaattCGATTTTAGGTTTTGGTCCAGTTCTCCCTTTAGCGGCATAATGTTTAATGCCGTACGAACCGCCACCGGCCAACCCCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                          <  2:1085048/133‑1 (MQ=255)                                                                                                acaggAATCGTTTTTATCATTTCGTTCAATTCTCCGTTTAGCGGCATACTGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                          <  2:399512/136‑1 (MQ=255)                                                                                                aGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                          <  1:236061/140‑1 (MQ=255)                                                                                                aGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                          <  1:1067455/140‑1 (MQ=255)                                                                                                aGCTGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATAGCGCTCTTCGTGTGTCGGGTCTGTTAAAAACTGgc                          >  2:593310/1‑140 (MQ=255)                                                                                                aGCTGAATCGATTTTATCATTTTGTTCAATTTCCCTTTTTGCGGCATATTGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                          <  2:700434/140‑1 (MQ=255)                                                                                                aGCTGAATCGATTTTATAATTTAGTTCAATTCTCCTTTTTGCGGCATAATGTTTAATGCCGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                          <  2:556102/140‑1 (MQ=255)                                                                                                   tGAATCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCt                       <  1:6762/140‑1 (MQ=255)                                                                                                     aaTCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTaa                     <  1:126797/140‑1 (MQ=255)                                                                                                     aaTCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTaa                     <  2:122557/140‑1 (MQ=255)                                                                                                     aaTCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTaa                     >  2:126797/1‑140 (MQ=255)                                                                                                     aaTCGATTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTaa                     >  1:1412446/1‑140 (MQ=255)                                                                                                     aaTCGATTTTATGATTTCGTTCTATTCTCCCCTTATCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGTGCTaa                     <  2:412506/140‑1 (MQ=255)                                                                                                          aTTTTATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATa                <  1:1337411/140‑1 (MQ=255)                                                                                                          aTTTTATGATTTGGTCCAATTCCCCGTTTTGCGGCATCATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATa                <  1:520508/140‑1 (MQ=255)                                                                                                           ttttATGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATAc               <  2:106258/140‑1 (MQ=255)                                                                                                               aTGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGGGTCGCGTGTGTGAAGAACTGGGGCTAACAATAcctgt           >  1:399512/1‑136 (MQ=255)                                                                                                                tGATTTGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCt          <  1:1431236/140‑1 (MQ=255)                                                                                                                     tGGTTCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTATGTAAAAAACTGCCGCTAACAATACAGGCTAAAGt     >  2:520508/1‑140 (MQ=255)                                                                                                                        ttCAATTCTCCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCGGTTAAAAACTGGCGCTAACAAGACAGGCTAAAGTCGa  >  1:428517/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                   TCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGA  >  NC_000913/2533449‑2533707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑